Fix in buffered glutaraldehyde for 2 hours, overnight is acceptable.
After multiple rinses, store in buffer in the refrigerator. Change the
buffer once a week, there are things that will grow in cacodylate
buffer. You may complete osmication if you want, then rinse and store in
buffer. I would not store the tissue indefinitely in anything. Do NOT
store the tissue in 70% ethanol, either before or after osmium, ethanol
has been shown to extract cytoplasm from fixed tissues.
The texts John Kiernan recommends are excellent.
Geoff
Nicholas David Evans wrote:
Dear all,
I would like to fix and preserve mouse skin tissue for processing for
transmission electron microscopy (TEM) at a later date. I was wondering
whether I can store tissue following fixation overnight in 2.5%
gluteraldehyde in 0.1M sodium cacodylate buffer and, if so, whether it can
be stored indefinitely in this buffer (or whether I need to treat with
OsO4 and store at 70% ethanol)?
Thanks and best wishes
Nick
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcaul...@umdnj.edu
**********************************************
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
