Hi Angelina,
We were having the same problem with frozen mouse aortas. I was cutting at 5ums onto superfrost slides and was losing about half of my sections. I ended up swapping to superfrost plus ultra, plus I bake the slides in the histology oven at 60 degress celcius for 10 minutes and now I nearly never lose a section - the only time I do is when I have done a lazy job of cutting and have collected a section with a fold in it. Good luck! > > Message: 9 > Date: Wed, 09 Dec 2009 11:40:50 -0800 > From: Angelina Fong <f...@zoology.ubc.ca> > Subject: [Histonet] Help! Losing sections from Superfrost Plus Slides > To: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: <4b1ffd42.2040...@zoology.ubc.ca> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Hi all, > > We have suddenly started losing tissue sections from our Superfrost Plus > Slides. > > Our students have been cutting fixed, frozen, cryosections (20um) and > thaw-mounting these onto Superfrost Plus slides. We have suddenly > started losing lots of sections from these slides (again!!). The tissue > is small - ie cross sections of frog aorta and longitudinal sections of > nerves, so any lose of adhesion results in total loss of the tissue. > The odd thing is that she is not losing every section on every slide, > but half to 3/4 of the sections are falling off within the first rinse. > The sections are from the same tissue block on the same slide while some > falls and others don't. > > We are at a lose as to what we can do to rescue these sections. > > Does anyone know if there is any way to coat the slides in some solution > with the tissue on them to help improve the adhesion without losing the > ability to do immunofluorescence? > > Any further advice on cutting / drying protocols are welcomed. > > This keeps happening and the inconsistency of it has us so frustrated > with this that we are thinking of going back to subbing our own slides. > > Thanks for your help! > > Angelina > > > -- > ~~o~~o~~o~~o~~o~~o~~o~~o~~o~~o > > Angelina Y. Fong, Ph.D. > Department of Zoology > Biological Sciences Building > 6270 University Boulevard > University of British Columbia > Vancouver, BC, V6T 1Z4 > Canada > > Ph: (604) 822-5799 > Fax: (604) 822-2416 > Email: f...@zoology.ubc.ca > > > > > > ------------------------------ > > Message: 10 > Date: Wed, 9 Dec 2009 15:08:57 -0500 > From: HistoLab <histosea...@gmail.com> > Subject: [Histonet] Help! Losing sections from Superfrost Plus Slides > To: histonet@lists.utsouthwestern.edu > Message-ID: > <521c6d260912091208n421bf467g3240121cd26f0...@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Angelina, > > I have seen this post a few times before and sometimes the problem was the > drying technique or making sure your water bath is clean and free of any > debris. I also remember hearing about someone pre-treating their slides with > a Trilogy (EDTA) buffer in a pressure cooker. > > Good Luck! > > *Matthew Semovoski* > Sales Manager > Gorilla Scientific Corporation > 1-866-435-4977 > www.gorillascientific.com _________________________________________________________________ Get more out of Hotmail Check out the latest features today http://windowslive.ninemsn.com.au/hotmail/article/878466/your-hotmail-is-about-to-get-even-better_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet