If this a frequent happening and the tissues stay an extra 30 to 60 minutes, it is not a big deal. You could to 2 things: 1- set the tissue processor "in delay" mode so it starts 30 to 60 minutes later,and by doing so the tissue will stay from 30-60 minutes extra in NBF, which is good, or 2- program in a way that the tissue will stay in paraffin 2 and 3 a total of 30-60 mins less, and your tissue will stay the same amount of time in paraffin as if they were not stuck in station 1. But, again, that is not a big deal. René J.
--- On Thu, 12/10/09, Dunlap, Charles <[email protected]> wrote: From: Dunlap, Charles <[email protected]> Subject: [Histonet] time tissue stays in paraffin To: [email protected] Date: Thursday, December 10, 2009, 10:56 AM We have a software glitch in our tissue processor, it stops in the first paraffin and will not progress to paraffins 2 and 3 then complete the cycle. If we catch it quickly, we can instruct it to continue but the cycle happens around 5:00 am so we sometimes are not here, tissuet may spend an extra 30 minutes or even an hour in the first paraffin. Estimated cost of repairing is a whopping 6 thousand $$. Does this have a major effect on quality of slides....sometimes ours are less than ideal but I am not sure if the problem is in the paraffin cycle or elsewhere...sectioning, staining, etc. Any ideas? Charles Dunlap _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
