Are fish scales even calcified? If so decalcifying may not work. If keratin - softening with ammonia water, Mollifex(sp?), Nair which is alkaline. I would be more tempted to use GMA plastic to match hardness of scales. I have a protocol for horse hooves. If you want will send privately. This is also in HistoLogic, author is Jane Chladny. Gayle Callis Sent from my Verizon Wireless BlackBerry
-----Original Message----- From: "Perry, Margaret" <margaret.pe...@sdstate.edu> Date: Thu, 10 Dec 2009 12:32:09 To: histonet@lists.utsouthwestern.edu<histonet@lists.utsouthwestern.edu> Subject: [Histonet] fish scales We are trying to cut fish scales that have been decalcified. They are chunking out when we try to cut them. I think we need to soften the keratin and I looked in the archives for the right dilution of ammonium hydroxide to use. One post said 5% the other said straight. What dilution do you recommend? I'm think it would probably be the same as what you use on toenails. Margaret Perry HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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