I have a Leica Bond and I have had a couple of small issues but I think that is par for the course with any instrument. Nothing is full proof. I run a small lab that processes 20,000 surgicals a year. The Bond seemed more economical for my situation without compromising quality. I have had the instrument for 1.5 years and I would purchase another.
>>> <histonet-requ...@lists.utsouthwestern.edu> 12/11/2009 1:05 pm >>> Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: fish scales (Josie Britton) 2. RE: Leica vs Ventana (Karin Groeger) 3. alternates to absolute ethanol (Michael W. Folsom) 4. RE: maximum block cutting (Rene J Buesa) 5. Re: alternates to absolute ethanol (Rene J Buesa) 6. one more question about reagent alcohol (Michael W. Folsom) 7. RE: alternates to absolute ethanol (Owen, Michael P) 8. Re: maximum block cutting (Rene J Buesa) 9. RE: alternates to absolute ethanol (Rene J Buesa) 10. Re: maximum block cutting (jel...@yumaregional.org) 11. Re: Leica vs Ventana (Akemi Allison) ---------------------------------------------------------------------- Message: 1 Date: Fri, 11 Dec 2009 10:34:46 -0500 From: "Josie Britton" <jcbrit...@cheshire-med.com> Subject: RE: [Histonet] fish scales To: "Josie Britton" <jcbrit...@cheshire-med.com>, "Perry, Margaret" <margaret.pe...@sdstate.edu>, <histonet@lists.utsouthwestern.edu> Message-ID: <f644cd64b2313f43bb3d2496b454cdac038c2...@cmc-ex01.cheshire-med.com> Content-Type: text/plain; charset="us-ascii" Oh, I forgot to mention we do this after we face the block. We put a little swirl on the block face and let it soak for 10-30+ minutes depending on spec. You can usually get a ribbon, then you have to re-soak for another ribbon. I hope this helps! Josie Britton HT -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Josie Britton Sent: Friday, December 11, 2009 10:31 AM To: Perry, Margaret; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] fish scales Nair hair remover works great on toenails! Josie Britton HT -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: Thursday, December 10, 2009 3:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fish scales We are trying to cut fish scales that have been decalcified. They are chunking out when we try to cut them. I think we need to soften the keratin and I looked in the archives for the right dilution of ammonium hydroxide to use. One post said 5% the other said straight. What dilution do you recommend? I'm think it would probably be the same as what you use on toenails. Margaret Perry HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ------------------------------ Message: 2 Date: Fri, 11 Dec 2009 07:48:36 -0800 From: "Karin Groeger" <kgroe...@uslabs.net> Subject: RE: [Histonet] Leica vs Ventana To: "Mahoney,Janice A" <janice.maho...@alegent.org>, "Houston, Ronald" <ronald.hous...@nationwidechildrens.org>, "Edwards,Richard E." <r...@leicester.ac.uk>, "Sebree Linda A" <lseb...@uwhealth.org>, "Kathleen Boozer" <booze...@ah.org>, <histonet@lists.utsouthwestern.edu> Message-ID: <f5085c791aaf6f47b0170d0b84fabccc051b9...@prodexch01.uslabs.int> Content-Type: text/plain; charset="us-ascii" We are a large Reference Lab and use both instruments and get good results and vendor support from Leica and Ventana, if you are a small lab then it should be your preference and what you feel comfortable with. Karin Groeger Histology Supervisor US LABS, Irvine,CA 949-450-0145 ext. 649 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Friday, December 11, 2009 7:02 AM To: 'Houston, Ronald'; Edwards,Richard E.; Sebree Linda A; Kathleen Boozer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica vs Ventana I think these obscure emails about vendors are very damaging, regardless of who the vender is. If someone is experiencing a particular problem with an instrument, please use this venue to get advice on how others handled it. Be open about the problem so that we all can learn from your experiences. To dog a company without any real information is not useful to anyone. Both companies have good instruments that may suit different types of labs better than the other. I'll get off my soap box now. Jan Mahoney, Omaha -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Friday, December 11, 2009 8:55 AM To: Edwards, Richard E.; Sebree Linda A; Kathleen Boozer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica vs Ventana Probably depends on whether you're talking about a white elephant or not Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: Edwards, Richard E. [mailto:r...@leicester.ac.uk] Sent: Friday, December 11, 2009 9:52 AM To: Houston, Ronald; Sebree Linda A; Kathleen Boozer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica vs Ventana This sounds like the game children play, so who would win the fight between an elephant and a hippopotamus, or the fight between a banana and a pomegranate etcetc. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: 11 December 2009 14:44 To: Sebree Linda A; Kathleen Boozer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica vs Ventana Not enough time in the day to answer that one Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Friday, December 11, 2009 9:41 AM To: Kathleen Boozer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica vs Ventana Why would you switch from a reliable, walk-away, reproducible system? Just curious. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kathleen Boozer Sent: Friday, December 11, 2009 8:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica vs Ventana Anyone out there found an advantage of going from Ventana to Leica for IHC? I am being warned about extra costs and protocol issues with the 3 trays. In negotiations.... Kathy Boozer, HT (ASCP), IHCQ Adventist Medical Center 10123 SE Market St. Portland, OR 97216 booze...@ah.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. 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The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This message, including any attachments, may contain CONFIDENTIAL AND/OR LEGALLY PRIVILEGED information. The information is intended for use by the individual named above and may not be disseminated to any other party without US LABS' written permission. If you are not the intended recipient, or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this information in error, please notify US LABS immediately at 1-888-450-0145 attn: Compliance Department to arrange for return of this message including all attachments. ------------------------------ Message: 3 Date: Fri, 11 Dec 2009 09:05:04 -0700 From: "Michael W. Folsom" <mwfol...@swcp.com> Subject: [Histonet] alternates to absolute ethanol To: histonet@lists.utsouthwestern.edu Message-ID: <4b226db0.5090...@swcp.com> Content-Type: text/plain; charset=ISO-8859-1 Hi: I want to process some plant tissue for microtomy. When I was doing this many years ago the protocol I used involved absolute ethanol. Knowing that getting ethanol that is really anhydrous is hard I was wondering if folks were using stuff that was denatured with 55% absolute methanol and 5% isoproponol instead. I understand it has better shelf life but wondered about the addition of small mounts of methanol and isopropanol. Also, if anybody is using this can you recommend a supplier? Thanks - Mike Rio Grand Biological ------------------------------ Message: 4 Date: Fri, 11 Dec 2009 08:47:45 -0800 (PST) From: Rene J Buesa <rjbu...@yahoo.com> Subject: RE: [Histonet] maximum block cutting To: histonet@lists.utsouthwestern.edu, histol...@medsurgpath.com, Cathy <cf...@007group.com>, ToniRathborne <trathbo...@somerset-healthcare.com> Message-ID: <190590.2820...@web65713.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Cutting : average of 2.5 min/block ( = 24/hour) Trimming: average of 25 secs./block ( =144/hour) RenΘ J. --- On Fri, 12/11/09, Rathborne, Toni <trathbo...@somerset-healthcare.com> wrote: From: Rathborne, Toni <trathbo...@somerset-healthcare.com> Subject: RE: [Histonet] maximum block cutting To: "Rene J Buesa" <rjbu...@yahoo.com>, histonet@lists.utsouthwestern.edu, histol...@medsurgpath.com, "Cathy" <cf...@007group.com> Date: Friday, December 11, 2009, 9:03 AM So what would the time be for trimming and cutting? -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Rene J Buesa Sent: Thursday, December 10, 2009 4:29 PM To: histonet@lists.utsouthwestern.edu; histol...@medsurgpath.com; Cathy Subject: RE: [Histonet] maximum block cutting Just actual cutting. Trimming is considered another task. RenΘ J. --- On Thu, 12/10/09, Cathy <cf...@007group.com> wrote: From: Cathy <cf...@007group.com> Subject: RE: [Histonet] maximum block cutting To: "'Rene J Buesa'" <rjbu...@yahoo.com>, histonet@lists.utsouthwestern.edu, histol...@medsurgpath.com Date: Thursday, December 10, 2009, 12:22 PM Does this average include trimming into the block or just the actual cutting of the section? Cathy -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, December 10, 2009 5:55 AM To: histonet@lists.utsouthwestern.edu; histol...@medsurgpath.com Subject: Re: [Histonet] maximum block cutting The productivity of a histotech is completely different with the number of slides a cytotech is allowed to screen daily. In the first case the final product is not affected by an increase of the productivity but in the second it is. A cytotech has to exert judgment while screening and could get easily tired compromising his/her diagnostic ability. Having said that, the cutting range for histotechs is from 5 to 70 blocks/houráwith an average of 24 (sample size = 245 histolabs). RenΘ J. --- On Wed, 12/9/09, histol...@medsurgpath.com <histol...@medsurgpath.com> wrote: From: histol...@medsurgpath.com <histol...@medsurgpath.com> Subject: [Histonet] maximum block cutting To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 9, 2009, 5:07 PM Hi Histonet, I know we have discussed the average blocks histotechs cut per hour, but does anyone know if there is a maximum blocks that can be cut per hour? Or per day? We have regulations for cytotechs who are reading slides for maximum slides they can read per hour and are wondering if there is any similar regulation for histotechs. Thank you all for you help, Katelin Katelin Lester, HTL (ASCP) MedSurg Pathology Associates, Inc. (503) 443-2157 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet á á á _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet á á á _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr ------------------------------ Message: 5 Date: Fri, 11 Dec 2009 09:03:10 -0800 (PST) From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] alternates to absolute ethanol To: histonet@lists.utsouthwestern.edu, "Michael W. Folsom" <mwfol...@swcp.com> Message-ID: <141761.25901...@web65715.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Pure isopropyl = isopropanol = 2-propanol is a better dehydrant than ethanol. Try it. RenΘ J. --- On Fri, 12/11/09, Michael W. Folsom <mwfol...@swcp.com> wrote: From: Michael W. Folsom <mwfol...@swcp.com> Subject: [Histonet] alternates to absolute ethanol To: histonet@lists.utsouthwestern.edu Date: Friday, December 11, 2009, 11:05 AM Hi: I want to process some plant tissue for microtomy.á When I was doing this many years ago the protocol I used involved absolute ethanol. Knowing that getting ethanol that is really anhydrous is hard I was wondering if folks were using stuff that was denatured with 55% absolute methanol and 5% isoproponol instead.á I understand it has better shelf life but wondered about the addition of small mounts of methanol and isopropanol. Also, if anybody is using this can you recommend a supplier? Thanks - Mike Rio Grand Biological _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 11 Dec 2009 10:14:21 -0700 From: "Michael W. Folsom" <mwfol...@swcp.com> Subject: [Histonet] one more question about reagent alcohol To: Histonet@lists.utsouthwestern.edu Message-ID: <4b227ded.6030...@swcp.com> Content-Type: text/plain; charset=ISO-8859-1 Folks: Thanks for the many quick responses to my question about using "reagent alcohol" instead of 100% ethanol. One further question - in plant histology its quite common to use alcohol in fixatives - i.e. FAA, FPA & Farmers. Does anybody have any experience in using 190 proof reagent alcohol in a fixative? I know it introduces small amounts of methanol and isopropanol into the "cocktail" but not sure if that really matters - Thanks for the advice/help! Mike Rio Grande Biological ------------------------------ Message: 7 Date: Fri, 11 Dec 2009 12:23:31 -0500 From: "Owen, Michael P" <michael.o...@fda.hhs.gov> Subject: RE: [Histonet] alternates to absolute ethanol To: "Histonet" <histonet@lists.utsouthwestern.edu> Message-ID: <6b1915839b67ad46b88e6be8f27376bb19f...@fmd3vs031.fda.gov> Content-Type: text/plain; charset="us-ascii" Dear Michael, Have you tried absolute methanol? The cost of the reagent should be less expensive than absolute ethanol and is easier to obtain than absolute ethanol (i.e. less legal restrictions than absolute ethanol that can be diverted to non-laboratory use). When fixing bacterial smears for a variety of staining methods, I have discovered absolute methanol works well and is often superior to heat fixation. Michael P. Owen, Regulatory Microbiologist U.S. FDA Pacific Regional Lab Northwest 22201 23rd Drive SE Bothell, WA 98021-4421 Phone: 425-483-4865 E-Mail: michael.o...@fda.hhs.gov ------------------------------ Message: 8 Date: Fri, 11 Dec 2009 09:36:01 -0800 (PST) From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] maximum block cutting To: histonet-boun...@lists.utsouthwestern.edu, histonet@lists.utsouthwestern.edu, histol...@medsurgpath.com, Cathy <cf...@007group.com>, ToniRathborne <trathbo...@somerset-healthcare.com>, jel...@yumaregional.org Message-ID: <80542.74357...@web65701.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 It is to be published in January 2010. If you "accept" 24 blocks/hour = 2.5 min/block RenΘ J. --- On Fri, 12/11/09, jel...@yumaregional.org <jel...@yumaregional.org> wrote: From: jel...@yumaregional.org <jel...@yumaregional.org> Subject: Re: [Histonet] maximum block cutting To: "Rene J Buesa" <rjbu...@yahoo.com>, histonet-boun...@lists.utsouthwestern.edu, histonet@lists.utsouthwestern.edu, histol...@medsurgpath.com, "Cathy" <cf...@007group.com>, "ToniRathborne" <trathbo...@somerset-healthcare.com> Date: Friday, December 11, 2009, 12:10 PM When was this data gathered... Some of this sounds excessive like 2.5 minutes a block??á Are these based on complete manual process or is automation also taken into consideration.á Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Rene J Buesa <rjbu...@yahoo.com> Date: Fri, 11 Dec 2009 08:47:45 To: <histonet@lists.utsouthwestern.edu>; <histol...@medsurgpath.com>; Cathy<cf...@007group.com>; ToniRathborne<trathbo...@somerset-healthcare.com> Subject: RE: [Histonet] maximum block cutting Cutting : average of 2.5 min/block ( = 24/hour) Trimming: average of 25 secs./block ( =144/hour) RenΘ J. --- On Fri, 12/11/09, Rathborne, Toni <trathbo...@somerset-healthcare.com> wrote: From: Rathborne, Toni <trathbo...@somerset-healthcare.com> Subject: RE: [Histonet] maximum block cutting To: "Rene J Buesa" <rjbu...@yahoo.com>, histonet@lists.utsouthwestern.edu, histol...@medsurgpath.com, "Cathy" <cf...@007group.com> Date: Friday, December 11, 2009, 9:03 AM So what would the time be for trimming and cutting? -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Rene J Buesa Sent: Thursday, December 10, 2009 4:29 PM To: histonet@lists.utsouthwestern.edu; histol...@medsurgpath.com; Cathy Subject: RE: [Histonet] maximum block cutting Just actual cutting. Trimming is considered another task. RenΘ J. --- On Thu, 12/10/09, Cathy <cf...@007group.com> wrote: From: Cathy <cf...@007group.com> Subject: RE: [Histonet] maximum block cutting To: "'Rene J Buesa'" <rjbu...@yahoo.com>, histonet@lists.utsouthwestern.edu, histol...@medsurgpath.com Date: Thursday, December 10, 2009, 12:22 PM Does this average include trimming into the block or just the actual cutting of the section? Cathy -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, December 10, 2009 5:55 AM To: histonet@lists.utsouthwestern.edu; histol...@medsurgpath.com Subject: Re: [Histonet] maximum block cutting The productivity of a histotech is completely different with the number of slides a cytotech is allowed to screen daily. In the first case the final product is not affected by an increase of the productivity but in the second it is. A cytotech has to exert judgment while screening and could get easily tired compromising his/her diagnostic ability. Having said that, the cutting range for histotechs is from 5 to 70 blocks/houráwith an average of 24 (sample size = 245 histolabs). RenΘ J. --- On Wed, 12/9/09, histol...@medsurgpath.com <histol...@medsurgpath.com> wrote: From: histol...@medsurgpath.com <histol...@medsurgpath.com> Subject: [Histonet] maximum block cutting To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 9, 2009, 5:07 PM Hi Histonet, I know we have discussed the average blocks histotechs cut per hour, but does anyone know if there is a maximum blocks that can be cut per hour? Or per day? We have regulations for cytotechs who are reading slides for maximum slides they can read per hour and are wondering if there is any similar regulation for histotechs. Thank you all for you help, Katelin Katelin Lester, HTL (ASCP) MedSurg Pathology Associates, Inc. (503) 443-2157 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet á á á _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet á á á _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law.á If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited.á If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ------------------------------ Message: 9 Date: Fri, 11 Dec 2009 09:40:15 -0800 (PST) From: Rene J Buesa <rjbu...@yahoo.com> Subject: RE: [Histonet] alternates to absolute ethanol To: Histonet <histonet@lists.utsouthwestern.edu>, Michael POwen <michael.o...@fda.hhs.gov> Message-ID: <436000.23257...@web65705.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 The concern with methanol would be its toxicity. Methanol (TWA=200 ppm)áis 5 times more toxic than ethanol (TWA=1000 ppm) and twice more toxic than 2-propanol (TWA=400ppm). RenΘ J. --- On Fri, 12/11/09, Owen, Michael P <michael.o...@fda.hhs.gov> wrote: From: Owen, Michael P <michael.o...@fda.hhs.gov> Subject: RE: [Histonet] alternates to absolute ethanol To: "Histonet" <histonet@lists.utsouthwestern.edu> Date: Friday, December 11, 2009, 12:23 PM Dear Michael, Have you tried absolute methanol? The cost of the reagent should be less expensive than absolute ethanol and is easier to obtain than absolute ethanol (i.e. less legal restrictions than absolute ethanol that can be diverted to non-laboratory use). When fixing bacterial smears for a variety of staining methods, I have discovered absolute methanol works well and is often superior to heat fixation. Michael P. Owen, Regulatory Microbiologist U.S. FDA Pacific Regional Lab Northwest 22201 23rd Drive SEá Bothell, WA 98021-4421 Phone: 425-483-4865á áááE-Mail: michael.o...@fda.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Fri, 11 Dec 2009 17:10:58 +0000 From: jel...@yumaregional.org Subject: Re: [Histonet] maximum block cutting To: "Rene J Buesa" <rjbu...@yahoo.com>, histonet-boun...@lists.utsouthwestern.edu, histonet@lists.utsouthwestern.edu, histol...@medsurgpath.com, "Cathy" <cf...@007group.com>, "ToniRathborne" <trathbo...@somerset-healthcare.com> Message-ID: <445413799-1260551459-cardhu_decombobulator_blackberry.rim.net-8569004...@bda047.bisx.prod.on.blackberry> Content-Type: text/plain; charset="Windows-1252" When was this data gathered... Some of this sounds excessive like 2.5 minutes a block?? Are these based on complete manual process or is automation also taken into consideration. Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Rene J Buesa <rjbu...@yahoo.com> Date: Fri, 11 Dec 2009 08:47:45 To: <histonet@lists.utsouthwestern.edu>; <histol...@medsurgpath.com>; Cathy<cf...@007group.com>; ToniRathborne<trathbo...@somerset-healthcare.com> Subject: RE: [Histonet] maximum block cutting Cutting : average of 2.5 min/block ( = 24/hour) Trimming: average of 25 secs./block ( =144/hour) RenΘ J. --- On Fri, 12/11/09, Rathborne, Toni <trathbo...@somerset-healthcare.com> wrote: From: Rathborne, Toni <trathbo...@somerset-healthcare.com> Subject: RE: [Histonet] maximum block cutting To: "Rene J Buesa" <rjbu...@yahoo.com>, histonet@lists.utsouthwestern.edu, histol...@medsurgpath.com, "Cathy" <cf...@007group.com> Date: Friday, December 11, 2009, 9:03 AM So what would the time be for trimming and cutting? -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Rene J Buesa Sent: Thursday, December 10, 2009 4:29 PM To: histonet@lists.utsouthwestern.edu; histol...@medsurgpath.com; Cathy Subject: RE: [Histonet] maximum block cutting Just actual cutting. Trimming is considered another task. RenΘ J. --- On Thu, 12/10/09, Cathy <cf...@007group.com> wrote: From: Cathy <cf...@007group.com> Subject: RE: [Histonet] maximum block cutting To: "'Rene J Buesa'" <rjbu...@yahoo.com>, histonet@lists.utsouthwestern.edu, histol...@medsurgpath.com Date: Thursday, December 10, 2009, 12:22 PM Does this average include trimming into the block or just the actual cutting of the section? Cathy -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, December 10, 2009 5:55 AM To: histonet@lists.utsouthwestern.edu; histol...@medsurgpath.com Subject: Re: [Histonet] maximum block cutting The productivity of a histotech is completely different with the number of slides a cytotech is allowed to screen daily. In the first case the final product is not affected by an increase of the productivity but in the second it is. A cytotech has to exert judgment while screening and could get easily tired compromising his/her diagnostic ability. Having said that, the cutting range for histotechs is from 5 to 70 blocks/houráwith an average of 24 (sample size = 245 histolabs). RenΘ J. --- On Wed, 12/9/09, histol...@medsurgpath.com <histol...@medsurgpath.com> wrote: From: histol...@medsurgpath.com <histol...@medsurgpath.com> Subject: [Histonet] maximum block cutting To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 9, 2009, 5:07 PM Hi Histonet, I know we have discussed the average blocks histotechs cut per hour, but does anyone know if there is a maximum blocks that can be cut per hour? Or per day? We have regulations for cytotechs who are reading slides for maximum slides they can read per hour and are wondering if there is any similar regulation for histotechs. Thank you all for you help, Katelin Katelin Lester, HTL (ASCP) MedSurg Pathology Associates, Inc. (503) 443-2157 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet á á á _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet á á á _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. 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Thank You. ------------------------------ Message: 11 Date: Fri, 11 Dec 2009 10:53:08 -0700 From: Akemi Allison <akemiat3...@yahoo.com> Subject: Re: [Histonet] Leica vs Ventana To: "Mahoney,Janice A" <janice.maho...@alegent.org> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>, Sebree Linda A <lseb...@uwhealth.org>, "'Houston, Ronald'" <ronald.hous...@nationwidechildrens.org>, Kathleen Boozer <booze...@ah.org> Message-ID: <227d3462-0d2d-4971-a0a8-34e558583...@yahoo.com> Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed Hi Janice, I'm glad you brought up your point! It's amazing how people are. It just shows how narrow minded and unkind in their statements. Yes, Ventana is HUGE, but they have their faults as well! That being said, there is always room to build a better mouse trap and save money while doing it too! The new Bond is amazing and is capable of doing multiple IHC staining on (1) slide. It is also a open system. "Science never sleeps"! Thank you for speaking out, and hope you have a Wonderful Holiday Season! Akemi Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Dec 11, 2009, at 8:02 AM, Mahoney,Janice A wrote: > I think these obscure emails about vendors are very damaging, > regardless of who the vender is. If someone is experiencing a > particular problem with an instrument, please use this venue to get > advice on how others handled it. Be open about the problem so that > we all can learn from your experiences. To dog a company without > any real information is not useful to anyone. > Both companies have good instruments that may suit different types > of labs better than the other. > I'll get off my soap box now. > Jan Mahoney, Omaha > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- > boun...@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald > Sent: Friday, December 11, 2009 8:55 AM > To: Edwards, Richard E.; Sebree Linda A; Kathleen Boozer; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Leica vs Ventana > > Probably depends on whether you're talking about a white elephant > or not > > Ronnie Houston > Anatomic Pathology Manager > Nationwide Children's Hospital > Columbus OH 43205 > (614) 722 5450 > > -----Original Message----- > From: Edwards, Richard E. [mailto:r...@leicester.ac.uk] > Sent: Friday, December 11, 2009 9:52 AM > To: Houston, Ronald; Sebree Linda A; Kathleen Boozer; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Leica vs Ventana > > This sounds like the game children play, so who would win the fight > between an elephant and a hippopotamus, or the fight between a banana > and a pomegranate etcetc. > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of > Houston, > Ronald > Sent: 11 December 2009 14:44 > To: Sebree Linda A; Kathleen Boozer; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Leica vs Ventana > > Not enough time in the day to answer that one > > Ronnie Houston > Anatomic Pathology Manager > Nationwide Children's Hospital > Columbus OH 43205 > (614) 722 5450 > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree > Linda A > Sent: Friday, December 11, 2009 9:41 AM > To: Kathleen Boozer; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Leica vs Ventana > > Why would you switch from a reliable, walk-away, reproducible system? > > Just curious. > > Linda A. Sebree > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > DB1-223 VAH > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of > Kathleen > Boozer > Sent: Friday, December 11, 2009 8:08 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Leica vs Ventana > > > Anyone out there found an advantage of going from Ventana to Leica for > IHC? I am being warned about extra costs and protocol issues with > the 3 > trays. > > In negotiations.... > > > Kathy Boozer, HT (ASCP), IHCQ > Adventist Medical Center > 10123 SE Market St. > Portland, OR 97216 > booze...@ah.org > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ----------------------------------------- Confidentiality Notice: > The following mail message, including any attachments, is for the > sole use of the intended recipient(s) and may contain confidential > and privileged information. The recipient is responsible to > maintain the confidentiality of this information and to use the > information only for authorized purposes. 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Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Sponsored by Catholic Health Initiatives and Immanuel Health > Systems, Alegent Health is faithful to the healing ministry of > Jesus Christ, providing high quality care for the body, mind and > spirit of every person. > > The information contained in this communication, including > attachments, is confidential and private and intended only for the > use of the addressees. Unauthorized use, disclosure, distribution > or copying is strictly prohibited and may be unlawful. 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