Why?, and why introduce such an apparently unnecessary variable?. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer Campbell Sent: 16 December 2009 18:35 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] leaving IHC slides in wash buffer
Hi Everyone, I have yet another IHC-related question. So after antigen retrieval, and then allowing my slides too cool, I place them in wash buffer before loading them on to the autostainer. Would leaving the slides in the wash buffer in the fridge for an extended period of time (say, 1 week) before continuing with immunostaining affect staining quality in any way? Thank you, Jen Campbell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
