Hi. I'm working on a protocol for cutting monkey brain sections to be mounted on 2x3 slides. I've read about utilizing a sliding microtome but in short, have decided to use the 2x3 adapter for a standard Microm microtome. During microtomy I've noticed many wrinkles in the sections, particularly within the folds of the cerebellum. The wrinkles worsen as the sections float in the water bath (temp=38).
In troubleshooting, a co-worker suggests inadequate fixation. I on the other hand believe that the wrinkles relate to the Type R paraffin, which contains polymers as well as the use of the adapter versus the sliding microtome. Can anyone offer any first hand experience/guidance? Thanks _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
