Rebecca, There are many companies that will set up the lab from beginning to end, TBS, Inc. has this service. You can contact them at 919-384-9393.
Valantou Grover, HT/HTL(ASCP), PA, MBA Biosciences Product Line Manager Polysciences, Inc. 400 Valley Road Warrington, PA 18976 Fax: 1-800-343-3291 Phone number: 1-800-523-2575 X7418 Direct:1-215-488-7418 Cell phone: 1-215-409-8327 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 73, Issue 39 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Auto Reply (Baustin) 2. Part Time MOHS Tech Needed in Portland (Eric Weber) 3. Re: Recycling Formalin, Xylene, and Alcohol (rg...@bmnet.com) 4. 2x3 microtomy: Adapter vs Sliding Microtome (R C) 5. Re: Stain to differentiate Hemoglobin from Hemosiderin? (Robert Richmond) 6. LEICA 2055 AUTOCUT (Lucy Zong) 7. Re: Stain to differentiate Hemoglobin from Hemosiderin? (John Kiernan) 8. Probe for mouse Y chromosome for FISH (birnba...@asaf.health.gov.il) 9. FNA code (Demarinis, Carolyn) 10. Derm Lab (Eric Sulkosky) 11. Re: FNA code (dkb...@chs.net) 12. Re: Probe for mouse Y chromosome for FISH (Xipamanine Mkuze) 13. RE: LEICA 2055 AUTOCUT (Feher, Stephen) 14. Mississippi Histotechnology Homecoming (Jerry Santiago) ---------------------------------------------------------------------- Message: 1 Date: Tue, 29 Dec 2009 13:06:33 -0500 From: Baustin <baus...@cbgbiotech.com> Subject: [Histonet] Auto Reply To: <histonet@lists.utsouthwestern.edu> Message-ID: <1000171...@mail-server.cbgbiotech.com> Content-Type: text/plain; charset="utf-8" Please do not respond to this automatic e-mail reply. I will be out of the office until Monday, January 4, 2010. If you need to place an order, please email it to suppl...@cbgbiotech.com or fax it to 614-863-1676 Attn Ordering. If you need to place a credit card order, please call 1-800-941-9484 and dial ext 201 or 225. Please note that all orders have been confirmed. If you did not receive a fax confirmation, your order was not received by CBG. ------------------------------ Message: 2 Date: Tue, 29 Dec 2009 14:53:37 -0500 From: Eric Weber <erwe...@maxhealth.com> Subject: [Histonet] Part Time MOHS Tech Needed in Portland To: <histonet@lists.utsouthwestern.edu> Message-ID: <9d12d4ef30176d4f839eb47f3d0e843611faa...@exbk2.maxhealth.com> Content-Type: text/plain; charset="us-ascii" MOHs Tech needed at the Portland VAMC for a Part Time Basis. Please contact me if you would be interested. Details: Dermatology MOHS Health Technician The Portland VA Medical Center is in need of one part-time Dermatology MOHS technician for the Dermatology Clinic, Operative Care Program at the Portland VA Medical Center, 3710 SW US Veterans Hospital Road, Portland, OR 97239. Qualifications: Knowledge of basic methods and procedures of the Dermatology clinic. Candidate shall demonstrate skill and precision in use of the tools, materials and equipment for Dermatology clinic. Knowledge of specialized terminology used in the specialized area of the facility where the work is performed. Strong customer service skills; detail oriented; and adaptability to work in a fast paced work environment. Candidate will serve as technical expert in the operation and maintenance of specialized equipment and instruments. Experience: Basic computer knowledge. Candidate shall demonstrate experience and skill in cutting and preparing frozen sections of tissue in a Dermatology MOHS clinic. Familiarity with medical terminology and familiarity with routine laboratory values and abbreviations are required. Specialty Experience (a Plus but not required): Familiarity with the VA electronic medical record system (CPRS). Length of Assignment: One year, or longer. On-Call Hours: Not available Overtime: Not available Tour of Duty: Mondays for 8 hours, (7:30am to 3:30pm), Fridays for 5 hours (7:30 am to 12:30 pm). Eric Weber Maxim Government Services 7227 Lee DeForest Dr Columbia, MD 21046 phone: (410) 910-4942 toll free: (866) 260-9142 fax:(410) 953-8358 erwe...@maxhealth.com CONFIDENTIALITY NOTE: This e-mail message contains confidential, privileged or otherwise protected information intended solely for the addressee. Please do not read, copy, or disseminate it unless you are the intended addressee. If you have received it in error, please call us (collect) at (410) 910-1500 and ask to speak with the message sender. Also, we would appreciate your forwarding the message back to us and deleting it from your system. Thank you. This e-mail and all other electronic (including voice) communications from the sender's firm are for informational purposes only. No such communication is intended by the sender to constitute either an electronic record or an electronic signature or to constitute any agreement by the sender to conduct a transaction by electronic means. Any such intention or agreement is hereby expressly disclaimed unless otherwise specifically indicated. ------------------------------ Message: 3 Date: Tue, 29 Dec 2009 16:04:22 -0500 From: rg...@bmnet.com Subject: [Histonet] Re: Recycling Formalin, Xylene, and Alcohol To: histonet@lists.utsouthwestern.edu Message-ID: <ofa3e10728.1d58bc58-on8525769b.007307a4-8525769b.0073c...@bmnet.com> Content-Type: text/plain; charset=US-ASCII Andria, CBG is very reliable. I have one of the large capacity units called: Solv Solv. It's set up to run xylene on one side and alcohol on the other. Very convenient, no flushes to do. I don't recommend recycling formalin, to much fume exposure, testing, rebuffering, etc., but this unit will work for formalin too. Renee Grow, BA., HT (ASCP) rg...@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax You wrote: Message: 4 Date: Mon, 21 Dec 2009 14:19:36 -0500 From: "Evans, Andria B" <aev...@wellspan.org> Subject: [Histonet] Recycling Formalin, Xylene, and Alcohol To: <histonet@lists.utsouthwestern.edu> Message-ID: <a3e1dfcf072d6046aabf519ae828adb107bf6...@exch4.wellspan.org> Content-Type: text/plain; charset="iso-8859-1" I would like to know what everyone out in histoland is using to recycle there solutions. We are looking at switching out the procyclers (that are about 10 years old) to something else. Any Pros/Cons of current methods would be great. Thanks!! Andria B Evans, HTL(ASCP)CM Anatomic Pathology York Hospital 1001 S. George Street York, PA 17405 717-851-5006 ------------------------------ Message: 4 Date: Tue, 29 Dec 2009 14:16:04 -0800 From: R C <ruebenjcar...@gmail.com> Subject: [Histonet] 2x3 microtomy: Adapter vs Sliding Microtome To: histonet@lists.utsouthwestern.edu Message-ID: <2a926e3f0912291416u723bd5d4ya6b93e199b4a...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi. I'm working on a protocol for cutting monkey brain sections to be mounted on 2x3 slides. I've read about utilizing a sliding microtome but in short, have decided to use the 2x3 adapter for a standard Microm microtome. During microtomy I've noticed many wrinkles in the sections, particularly within the folds of the cerebellum. The wrinkles worsen as the sections float in the water bath (temp=38). In troubleshooting, a co-worker suggests inadequate fixation. I on the other hand believe that the wrinkles relate to the Type R paraffin, which contains polymers as well as the use of the adapter versus the sliding microtome. Can anyone offer any first hand experience/guidance? Thanks ------------------------------ Message: 5 Date: Tue, 29 Dec 2009 17:26:22 -0500 From: Robert Richmond <rsrichm...@gmail.com> Subject: [Histonet] Re: Stain to differentiate Hemoglobin from Hemosiderin? To: histonet@lists.utsouthwestern.edu Message-ID: <abea52a60912291426y68ea4080h94c2f627141a1...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Jerry Ricks, Research Scientist, University of Washington,Department of Pathology asks: >>I've seen Prussian blue as a stain for hemosiderin but that would also stain hemoglobin.<< The Perls prussian blue reaction occurs with hemosiderin ("stainable iron") but not with hemoglobin, hematin, formalin pigment, malarial pigment, or melanin. It isn't a stain, but a reaction between ferric iron and ferrocyanide ion that produces a dense blue precipitate. The Stainsfile page doesn't really answer your questions, and I'd suggest looking it up in some of the standard textbooks. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 6 Date: Tue, 29 Dec 2009 18:05:10 -0500 From: Lucy Zong <lucy.z...@gmail.com> Subject: [Histonet] LEICA 2055 AUTOCUT To: histonet@lists.utsouthwestern.edu Message-ID: <8daef62e0912291505l7721b8acw2c339e97a29dd...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 I was given a Leica 2055 microtome for our lab, however it did not come with an operators manual. Does anyone have one they could e-mail to me? Thank you ------------------------------ Message: 7 Date: Tue, 29 Dec 2009 20:37:52 -0500 From: John Kiernan <jkier...@uwo.ca> Subject: Re: [Histonet] Stain to differentiate Hemoglobin from Hemosiderin? To: JR R <rosenfeld...@hotmail.com> Cc: histonet@lists.utsouthwestern.edu Message-ID: <fc31fb4b684f7.4b3a6...@uwo.ca> Content-Type: text/plain; charset=windows-1252 Please explain. The iron in haemoglobin is tightly protein-bound and not stainable by histochemical methods for the iron in haemosiderin and ferritin. Red blood cells are, for example, Prussian-blue negative. John Kiernan UWO. London, Canada == == == ----- Original Message ----- From: JR R <rosenfeld...@hotmail.com> Date: Monday, December 28, 2009 19:54 Subject: [Histonet] Stain to differentiate Hemoglobin from Hemosiderin? To: histonet@lists.utsouthwestern.edu > > I've seen Prussian blue as a stain for hemosiderin but that > would also stain hemoglobin. > > Any ideas? > > Thanks, > > Jerry Ricks > Research Scientist > University of Washington > Department of Pathology > > _________________________________________________________________ > Hotmail: Trusted email with Microsofts powerful SPAM protection. > http://clk.atdmt.com/GBL/go/177141664/direct/01/____________________________ ___________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Wed, 30 Dec 2009 12:17:15 +0200 From: <birnba...@asaf.health.gov.il> Subject: [Histonet] Probe for mouse Y chromosome for FISH To: <histonet@lists.utsouthwestern.edu> Message-ID: <054452ccc076be4da21e46ae95e32ec3277...@mail2.asaf.health.gov.il> Content-Type: text/plain; charset="windows-1255" Dear all We look for Fluorescence probe of mouse Y chromosome. Which company does sale it? Mira Birnbaum Pathology Asaf Hrofeh Medical Center Israel ------------------------------ Message: 9 Date: Wed, 30 Dec 2009 08:17:46 -0500 From: "Demarinis, Carolyn" <cdemari...@saratogacare.org> Subject: [Histonet] FNA code To: <histo...@pathology.swmed.edu> Message-ID: <f15f698e03068a4ca11d19d3e0ed2815066e2...@shexch1.saratogacare.org> Content-Type: text/plain; charset="us-ascii" Which CPT code are labs using for fine needle aspirations that are processed using thinprep technique - FNA interpretation and report-88173 or thinprep non-gyn 88112? Thank you. This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at priv...@saratogacare.org and destroy all copies of this communication and any attachments. ------------------------------ Message: 10 Date: Wed, 30 Dec 2009 09:40:09 -0500 From: Eric Sulkosky <esulko...@gmail.com> Subject: [Histonet] Derm Lab To: histonet@lists.utsouthwestern.edu Message-ID: <6c3840890912300640p70132627w9befa4fcca151...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 RAJ, What tips are you looking for? Are you starting from scratch with an empty room or has the room already been equipped with ventilation, plumbing and electrical? Eric ------------------------------ Message: 11 Date: Wed, 30 Dec 2009 09:42:03 -0500 From: dkb...@chs.net Subject: Re: [Histonet] FNA code To: "Demarinis, Carolyn" <cdemari...@saratogacare.org> Cc: histonet-boun...@lists.utsouthwestern.edu, histo...@pathology.swmed.edu Message-ID: <of9c7d5ee1.697484ab-on8525769c.0050bc7a-8525769c.0050b...@chs.net> Content-Type: text/plain; charset="US-ASCII" 88173 Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net "Demarinis, Carolyn" <cdemari...@saratogacare.org> Sent by: histonet-boun...@lists.utsouthwestern.edu 12/30/2009 08:18 AM To <histo...@pathology.swmed.edu> cc Subject [Histonet] FNA code Which CPT code are labs using for fine needle aspirations that are processed using thinprep technique - FNA interpretation and report-88173 or thinprep non-gyn 88112? Thank you. This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at priv...@saratogacare.org and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 12 Date: Wed, 30 Dec 2009 16:37:58 +0100 From: Xipamanine Mkuze <xipaman...@gmail.com> Subject: Re: [Histonet] Probe for mouse Y chromosome for FISH To: birnba...@asaf.health.gov.il Cc: histonet@lists.utsouthwestern.edu Message-ID: <3cfdeb590912300737g155fbd26v1e2a7b0ed98d3...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Cambio (http://www.cambio.co.uk) 2009/12/30 <birnba...@asaf.health.gov.il> > Dear all > > We look for Fluorescence probe of mouse Y chromosome. Which company does > sale it? > > Mira Birnbaum > > Pathology > > Asaf Hrofeh Medical Center > > Israel > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 13 Date: Wed, 30 Dec 2009 11:43:31 -0500 From: "Feher, Stephen" <sfe...@cmc-nh.org> Subject: RE: [Histonet] LEICA 2055 AUTOCUT To: "Lucy Zong" <lucy.z...@gmail.com>, <histonet@lists.utsouthwestern.edu> Message-ID: <73a7ed895ee0c24d9267ed814911df1912b74...@exchange.cmc-nh.org> Content-Type: text/plain; charset="us-ascii" I have one for the RM2255 if you think that will help. Steve -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lucy Zong Sent: Tuesday, December 29, 2009 6:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] LEICA 2055 AUTOCUT I was given a Leica 2055 microtome for our lab, however it did not come with an operators manual. Does anyone have one they could e-mail to me? Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Wed, 30 Dec 2009 09:08:15 -0800 (PST) From: Jerry Santiago <jsanti...@bellsouth.net> Subject: [Histonet] Mississippi Histotechnology Homecoming To: histonet@lists.utsouthwestern.edu Message-ID: <861067.47883...@web180409.mail.gq1.yahoo.com> Content-Type: text/plain; charset=utf-8 Mississippi Histotechnology Homecoming Calling all Histotechnologists from the State of Mississippi. The awaited homecoming for the Mississippi Society of Histotechnology is finally here.B The meeting information will be available in January 2010. To receive the information, please send an e-mail with name and address to my attention to: jsanti...@bellsouth.net. Event: Mississippi Histotechnology Homecoming Event Dates: March 12 b _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet