Hi Meghan, Healthy lung is terrible to cut frozen. All those air spaces collapse on sectioning. Unhealthy lung on the other hand is a dream to cut! I have heard it suggested to perfuse the lungs with dilute frozen cutting compound (eg OCT or similar) prior to selecting the piece(s) for frozen sectioning. In theory it should work well but I have not tried it myself. Otherwise, a quick swipe of the cutting surface with your thumb just before sectioning might help a little (maximizing the amount of viewable tissue in the section) but your sections (of healthy lung)will never be "beautiful" in my experience. good luck. Greg
Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> Meghan Tucker <meghan.tuc...@yahoo.com> 1/4/2010 11:23:01 AM >>> Hello! I am looking for some helpful hints for cutting frozen lung tissue. I have tried just about everything I can think of! Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
