Hi All, Happy New Year... As the new year is upon us I and my fellow histologist feel compelled to attempt IHC on mouse tissue again for the detection of various cytokines. As I have not had much success in this area in the past I thought I would ask if there is anyone out there that you know that may help us on our Journey..
We want to try to detect cytokine (TNF,IL-1,IL-13) along with our soluble targets in mouse and rat tissues. Ideally we want to use paraffin sections so we will explore fixations/processing schedules..and any other voodoo solutions that might work. If you or someone you know has a good source of papers/ protocol we might download we would love to have them.. As we have the ability to use mice and rats we will be simulating/overexpression of cytokines in-vivo and taking tissues at necropsies in various fixations. If you can help please let me know. Thanks and Happy new Year. Jamie Erickson Abbott Labs. Scientist II, M.S. HTL (ASCP) Discovery Safety, Metabolism & Pharmacokinetics Phone 508-688-3134 FAX 508-793-4895 jamie.erick...@abbott.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet