We use formic acid (10%) for decalcification. We fix in 10% NBF for 48 hours. We decal for about 7 days for the mouse joints (ankles, front and rear paws), knees do not take as long.
Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Igor Deyneko Sent: Monday, January 04, 2010 12:17 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Decaling mouse joints Dear Histonetters! I have a project coming up which would require to decalcify mouse hind legs, femur and tibia with joints. Can anyone suggest a good system, either an ion exchange or acid decal(formic acid I've heard works well) and also if anyone has a protocol on fixation and decaling, it would be much appreciated. Thank you. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet