Hi, I have had a problem with "splotchy" elastin staining on certain sections of mouse aorta. I use the Modified Movats and do not use a microwave. But from this one lab some areas of the elastic fibers will retain the hematolylin after differentiation while some areas lose it completely, all in the same section.
They fix in 10%NBF and the processing gets done at their lab and I receive the unstained slides. My control does not have this problem. Any ideas? Thanks, Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
