Hello, I have a question regarding detecting injected mouse antibody in mouse tissue by IHC. Now I have mouse liver paraffin section. This mouse was injected mouse antibody. I was asked to locate this antibody. This is a mouse chimeric antibody. Variable regions from human antibody was combined with mouse IgG2a heavy chain and kappa light chain constant regions. I am thinking using rabbit anti-human IgG, F(ab) fragment specific. Do you think it's doable or you have any good suggestion? When this mouse antibody bind to antigen inside liver through this human variable region, my F(ab) fragment specific antibody can still bind to it? Hope I describ my questions clearly. Thank you in advance for any help! Amy
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