IF you can get hold of it, a phase microscope will be the best solution. You could also try to use "oblique" illumination by off-centering the condenser diaphragm. René J.
--- On Tue, 1/26/10, Reno Genest <rgen...@uwaterloo.ca> wrote: From: Reno Genest <rgen...@uwaterloo.ca> Subject: [Histonet] Simple dye for cornea and sclera. To: histonet@lists.utsouthwestern.edu Date: Tuesday, January 26, 2010, 10:36 AM Hello everyone, I am trying to reconstruct the 3D geometry of the cornea and sclera of a chicken eye. I embedded and froze an eye using OCT and CO2 liquid withdrawal and sliced it using a microtome. I took pictures from the top of the eye (not the sections but what is left after slicing) using a digital camera. The problem is that the sclera and cornea are difficult to see. Is there a dye I could use before taking a picture that can be applied in one step and do not require a lengthy protocol? I cannot afford to wash the eye with tap water because it is still frozen on the microtome. Also, is anyone aware of a dye that would permeate through the sclera and cornea so that I could just dip the eye in before slicing it? Thank you. Reno Genest MSc. candidate Mechanical engineering University of Waterloo _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
