It also may depend on the fluorophore you are using (you did not say???) John Kiernan's reply was excellent but if you are using Alexa or Dylight dyes, you should contact the technical services at Molecular Probes for Alexa and whoever makes the Dylight fluorophore since Histonet replies are mixed. Jackson Immunoresearch now supplies antibodies, etc conjugated to various Dylights, and their technical services are excellent for helping you.
Gayle M. Callis HTL/HT/MT(ASCP) -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Emily Sours Sent: Monday, February 01, 2010 11:18 AM To: [email protected] Subject: [Histonet] fluorescence with xylene dehydration Hello Has anyone used a xylene dehydration at the end of fluorescent staining? I've searched the histonet archives for this answer but the answers were mixed. I thought I'd ask again to see if there were any new opinions. Emily __________ Information from ESET Smart Security, version of virus signature database 4824 (20100201) __________ The message was checked by ESET Smart Security. http://www.eset.com _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
