Check pH of your washing water.
pH too low will result in not staining at all.

I'd clean the stainer as well.


> Hello Histonetters
>
> We are having trouble with light staining from the hematoxylin.  It is the 
> same lot number we have been in.  We changed it out and still the same.  All 
> reagent containers were emptied, cleaned and refilled yesterday.  We have not 
> changed anything with the timings on our stainer.  What am I missing?
>
> Thanks for your help
> Nancy Schmitt
> United Clinical Laboratories
> Dubuque, IA
>
>
>
>
> NOTICE: This email may contain legally privileged information. The information
> is for the use of only the intended recipient(s) even if addressed
> incorrectly. If you are not the intended recipient, please notify the sender
> that you have received it in error and then delete it along with any
> attachments. Thank you.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>   


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Reply via email to