Check pH of your washing water. pH too low will result in not staining at all.
I'd clean the stainer as well. > Hello Histonetters > > We are having trouble with light staining from the hematoxylin. It is the > same lot number we have been in. We changed it out and still the same. All > reagent containers were emptied, cleaned and refilled yesterday. We have not > changed anything with the timings on our stainer. What am I missing? > > Thanks for your help > Nancy Schmitt > United Clinical Laboratories > Dubuque, IA > > > > > NOTICE: This email may contain legally privileged information. The information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet