Hi every body We want to scan fish spermatozoa By SEM. But I don't know the method for dehydration after Post fixation with osmium tetra oxide? By ethanol series or aceton? I thanks to all who can help me in SEM for Spermatozoa.
Regards: Jamshid -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 75, Issue 20 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Hi to all............. (Don Hammer) 2. IHC TBS wash buffer (Justin Peters) 3. Florida Society for Histotechnology-Region III Meeting (Jerry Santiago) 4. QIHC (Walter Benton) 5. FW: FYI - Histonet Digest, Vol 75, Issue 17 (Fawn Bomar) 6. FW: FYI - Histonet Digest, Vol 75, Issue 17 (Fawn Bomar) 7. Re: IHC TBS wash buffer (V. Neubert) 8. November 2009 copy of ASCP Lab Medicine journal (Justin Peters) 9. Re: November 2009 copy of ASCP Lab Medicine journal (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Sun, 14 Feb 2010 22:02:27 -0800 From: "Don Hammer" <do...@earthlink.net> Subject: [Histonet] Hi to all............. To: "histonet" <histonet@lists.utsouthwestern.edu> Message-ID: <281414d805204848ba1cc3d07ab02...@don> Content-Type: text/plain; charset="iso-8859-1" Hi Histology Professionals, I just finished reading the recent issue of "NSH In Action", noting a reference to "Histonet" so thought to drop in to see how this very important, long lasting exchange of information is going. Congrats Linda with thanks to the UTSouthwestern for supporting the site. (14 years now I believe) Perhaps there are a few colleagues still here, continuing to offer assistance from their wealth of experience and knowledge, from back in my Laboratory days (or was that Daze?) :>) It's been near 12 years since retiring, wouldn't give it up for anything, but often think of you all who are in the Profession and now preparing for "Histotechnology Professionals Day" Such a great opportunity to blow your own horn a bit while making the general public aware of your support in the various facets of Medicine. Don't forget to celebrate all you do on a daily basis. I'm completely blown away at how NSH has grown from our Founding Meeting 36 years ago with it's extensive Symposium/Convention, Educational Materials, Journal of Histotechnology, Summer Symposium's, Teleconferences, Specialty Forums, Awards, Positions with or on Boards of National Organizations, just to highlight a few. When viewing the Website containing so much info for Members it's hard to believe much of it was kept on Card files and File drawers manned by one person in the Office. What's difficult to understand is the total Membership is nearly the same number. Well just sending some thoughts. Think I figured out how to post a comment, now I need to go figure out how to view the daily posts on "Histonet" It's been so long since doing that. Don Hammer, Retired Guy " I can't remember the last time I wasn't at least kind of tired." "I totally take back all those times I didn't want to nap when I was younger" ------------------------------ Message: 2 Date: Mon, 15 Feb 2010 08:24:41 -0500 From: "Justin Peters" <jpet...@bostwicklaboratories.com> Subject: [Histonet] IHC TBS wash buffer To: <histonet@lists.utsouthwestern.edu> Message-ID: <24d22de9e488aa43bf92a4389f2ddb1f0eca0...@mail1.bostwick.com> Content-Type: text/plain; charset="us-ascii" Can anyone tell me what an acceptable pH range for IHC TBS wash buffer is? ------------------------------ Message: 3 Date: Mon, 15 Feb 2010 05:29:08 -0800 (PST) From: Jerry Santiago <jsanti...@bellsouth.net> Subject: [Histonet] Florida Society for Histotechnology-Region III Meeting To: histonet@lists.utsouthwestern.edu Message-ID: <967631.97107...@web180415.mail.gq1.yahoo.com> Content-Type: text/plain; charset=us-ascii Dear Histonetters, The program and online registration for the Florida Society for Histotechnology Meeting is now available at www.fshgroup.org. You can also go directly to the online registration process by visiting www.fshmeeting.com. Sincerely, Jerry Santiago, BS, HTL(ASCP)QIHC NSH Region III Director Shands Jacksonville T: 904-244-6149 E-mail: sant...@bellsouth.net ------------------------------ Message: 4 Date: Mon, 15 Feb 2010 09:13:14 -0500 From: "Walter Benton" <wben...@umm.edu> Subject: [Histonet] QIHC To: <histonet@lists.utsouthwestern.edu> Message-ID: <4b790fc5.d886.00f...@umm.edu> Content-Type: text/plain; charset=US-ASCII I recently took and passed the examination . I used the Dako book, material from Ethel Macrea's presentation, NSH study guide and material from the other references on the list. Ethel can be reached at emac...@swskinpath.com She is a wonderful resource. Message: 6 Date: Fri, 12 Feb 2010 20:22:01 -0500 From: jfi...@aol.com Subject: [Histonet] QIHC To: histonet@lists.utsouthwestern.edu Message-ID: <8cc7a6213f16820-47fc-8...@webmail-m018.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Hello, I was wondering if anyone out there had any tips on passing the QIHC, I have studied with the Dako book but unfortunately I did not pass at my first attempt. If there are any sample questions or packages available I would really like to get them. Thanks fj Walter Benton, HT(ASCP)QIHC Histology Supervisor University of Maryland Medical Center Anatomic Pathology 22 S. Greene St Room NBW65 Baltimore MD 21201 (Direct) 410-328-0930 (Lab) 410-328-5524 (Fax) 410-328-5508 This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. ------------------------------ Message: 5 Date: Mon, 15 Feb 2010 09:24:26 -0500 From: "Fawn Bomar" <fawn.bo...@halifaxregional.com> Subject: [Histonet] FW: FYI - Histonet Digest, Vol 75, Issue 17 To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <e779667a9118f74d8b7d82a1891be0300274fcd...@exch-2k7.hrhs.com> Content-Type: text/plain; charset=us-ascii Hi everyone, Since I received this e-mail from Lynne about a question that I asked, I decided it was probably in my best interest not to ask any more questions in general. Thank you to everyone that helped verify that my validation process was indeed up to par! I will stay on the histonet in hopes that I can help others but I will keep my own questions to myself Fawn ________________________________________ From: Jones, Lynne [jone...@mir.wustl.edu] Sent: Friday, February 12, 2010 5:48 PM To: Fawn Bomar Subject: FYI - Histonet Digest, Vol 75, Issue 17 Hello - I was curious about the facility where you worked, so I looked up Halifax Regional, but it seems congratulations are in order, since Gwen Wade just posted a Press release indicating that the lab passed CAP accreditation. (Though I'm not sure your inquiry about a fundamental process in a histology lab would inspire confidence.) HistoNet is a fabulous resource, but there are some questions you may want to ask using a less traceable e-mail account. HistoNet archives are readily accessible, even to the general public. I do understand the panic and second-guessing that hits right before the inspectors appear, but you obviously had all the I's dotted and t's crossed. The next time will be easier. :) Take care, Lynne -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet- ------------------------------ Message: 3 Date: Thu, 11 Feb 2010 14:06:56 -0500 From: "Fawn Bomar" <fawn.bo...@halifaxregional.com> Subject: [Histonet] Help... To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <e779667a9118f74d8b7d82a1891be0300274fcd...@exch-2k7.hrhs.com> Content-Type: text/plain; charset=iso-8859-1 Does anyone have a protocol or procedure for validating the Her2 antibody? We are trying to get ready for a CAP inspection and I'm not sure if what we did is ok or not. Thank you in advance Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you ------------------------------ Message: 4 Date: Thu, 11 Feb 2010 12:28:27 -0700 From: "Liz Chlipala" <l...@premierlab.com> Subject: RE: [Histonet] Help... To: "Fawn Bomar" <fawn.bo...@halifaxregional.com>, <histonet@lists.utsouthwestern.edu> Message-ID: <ee33be5c905a3046a7ff8f58a64c8e4b100...@server.premierlab.local> Content-Type: text/plain; charset="us-ascii" Fawn CAP has specific quidelines, its right there in the questions, did you do what that question is asking and have you documented what was done. They tell you right in the check sheet what needs to be completed. Test validation must be performed on a minimum of 25 cases (they recommend 25- 100) and they tell you how to validate too. There is also an article that you can get on the CAP website on IHC standardization. I have a pdf of it here if you need it. LIz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fawn Bomar Sent: Thursday, February 11, 2010 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help... Does anyone have a protocol or procedure for validating the Her2 antibody? We are trying to get ready for a CAP inspection and I'm not sure if what we did is ok or not. Thank you in advance Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 75, Issue 17 **************************************** The material in this message is private and may contain Protected Healthcare Information (PHI). If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return mail. ------------------------------ Message: 6 Date: Mon, 15 Feb 2010 09:29:45 -0500 From: "Fawn Bomar" <fawn.bo...@halifaxregional.com> Subject: [Histonet] FW: FYI - Histonet Digest, Vol 75, Issue 17 To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <e779667a9118f74d8b7d82a1891be0300274fcd...@exch-2k7.hrhs.com> Content-Type: text/plain; charset=us-ascii One more thing.... This was my reply to Lynn and her response to my question. Fawn ________________________________________ From: Fawn Bomar Sent: Monday, February 15, 2010 9:24 AM To: Jones, Lynne Subject: RE: FYI - Histonet Digest, Vol 75, Issue 17 Hi Lynne, My inquiry about the Her2 validation was to get overall suggestions on how different labs interpreted how to go about the validation process. I said that we had a process but since I was new to the clinical aspect and to immuno's in general, I wanted to make sure that we were up to par with everyone else or if I needed to make adjustments to make our validation better. I was making sure that we met or exceeded expectations and I felt that everyone could offer an opinion. There should be no doubt in confidence in our accreditation...there should be some comfort in knowing that I am making sure that we are doing the best work that can be done. Sincerely Fawn ________________________________________ From: Jones, Lynne [jone...@mir.wustl.edu] Sent: Friday, February 12, 2010 5:48 PM To: Fawn Bomar Subject: FYI - Histonet Digest, Vol 75, Issue 17 Hello - I was curious about the facility where you worked, so I looked up Halifax Regional, but it seems congratulations are in order, since Gwen Wade just posted a Press release indicating that the lab passed CAP accreditation. (Though I'm not sure your inquiry about a fundamental process in a histology lab would inspire confidence.) HistoNet is a fabulous resource, but there are some questions you may want to ask using a less traceable e-mail account. HistoNet archives are readily accessible, even to the general public. I do understand the panic and second-guessing that hits right before the inspectors appear, but you obviously had all the I's dotted and t's crossed. The next time will be easier. :) Take care, Lynne -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet- ------------------------------ Message: 3 Date: Thu, 11 Feb 2010 14:06:56 -0500 From: "Fawn Bomar" <fawn.bo...@halifaxregional.com> Subject: [Histonet] Help... To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <e779667a9118f74d8b7d82a1891be0300274fcd...@exch-2k7.hrhs.com> Content-Type: text/plain; charset=iso-8859-1 Does anyone have a protocol or procedure for validating the Her2 antibody? We are trying to get ready for a CAP inspection and I'm not sure if what we did is ok or not. Thank you in advance Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you ------------------------------ Message: 4 Date: Thu, 11 Feb 2010 12:28:27 -0700 From: "Liz Chlipala" <l...@premierlab.com> Subject: RE: [Histonet] Help... To: "Fawn Bomar" <fawn.bo...@halifaxregional.com>, <histonet@lists.utsouthwestern.edu> Message-ID: <ee33be5c905a3046a7ff8f58a64c8e4b100...@server.premierlab.local> Content-Type: text/plain; charset="us-ascii" Fawn CAP has specific quidelines, its right there in the questions, did you do what that question is asking and have you documented what was done. They tell you right in the check sheet what needs to be completed. Test validation must be performed on a minimum of 25 cases (they recommend 25- 100) and they tell you how to validate too. There is also an article that you can get on the CAP website on IHC standardization. I have a pdf of it here if you need it. LIz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fawn Bomar Sent: Thursday, February 11, 2010 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help... Does anyone have a protocol or procedure for validating the Her2 antibody? We are trying to get ready for a CAP inspection and I'm not sure if what we did is ok or not. Thank you in advance Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 75, Issue 17 **************************************** The material in this message is private and may contain Protected Healthcare Information (PHI). If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return mail. ------------------------------ Message: 7 Date: Mon, 15 Feb 2010 15:55:00 +0100 From: "V. Neubert" <histonet.nos...@vneubert.com> Subject: Re: [Histonet] IHC TBS wash buffer To: histonet@lists.utsouthwestern.edu Message-ID: <4b796044.9080...@vneubert.com> Content-Type: text/plain; charset=ISO-8859-1 7,6 ;) > Can anyone tell me what an acceptable pH range for IHC TBS wash buffer > is? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 8 Date: Mon, 15 Feb 2010 10:45:35 -0500 From: "Justin Peters" <jpet...@bostwicklaboratories.com> Subject: [Histonet] November 2009 copy of ASCP Lab Medicine journal To: <histonet@lists.utsouthwestern.edu> Message-ID: <24d22de9e488aa43bf92a4389f2ddb1f0eca0...@mail1.bostwick.com> Content-Type: text/plain; charset="us-ascii" Does anyone have a copy of the November 2009 ASCP Lab Medicine journal that they wouldn't mind parting with? I have a colleague who's wife had a paper published in that edition and he would like to have a copy. Thanks! Justin Peters, HTL (ASCP) 4355 Innslake Drive Glen Allen, Virginia 23060 Phone: (804) 967-9225 ext. 1831 Cell: (804) 822-6084 ------------------------------ Message: 9 Date: Mon, 15 Feb 2010 07:50:18 -0800 (PST) From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] November 2009 copy of ASCP Lab Medicine journal To: histonet@lists.utsouthwestern.edu, Justin Peters <jpet...@bostwicklaboratories.com> Message-ID: <288692.27975...@web65708.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Just out of curiosity: isn't your colleague's wife entitled to a copy of the issue where she published the article? Reni J. --- On Mon, 2/15/10, Justin Peters <jpet...@bostwicklaboratories.com> wrote: From: Justin Peters <jpet...@bostwicklaboratories.com> Subject: [Histonet] November 2009 copy of ASCP Lab Medicine journal To: histonet@lists.utsouthwestern.edu Date: Monday, February 15, 2010, 10:45 AM Does anyone have a copy of the November 2009 ASCP Lab Medicine journal that they wouldn't mind parting with? I have a colleague who's wife had a paper published in that edition and he would like to have a copy. Thanks! Justin Peters, HTL (ASCP) 4355 Innslake Drive Glen Allen, Virginia 23060 Phone: (804) 967-9225 ext. 1831 Cell: (804) 822-6084 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 75, Issue 20 **************************************** __________ Information from ESET NOD32 Antivirus, version of virus signature database 4869 (20100215) __________ The message was checked by ESET NOD32 Antivirus. http://www.eset.com __________ Information from ESET NOD32 Antivirus, version of virus signature database 4869 (20100215) __________ The message was checked by ESET NOD32 Antivirus. http://www.eset.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet