We normally do CG's and DRG's in Frozen format as well and have had
difficulty working with histogel and low percetages of ethanol. The
histogel doesn't really bond with the samples very well when taken from an
low % ethanol for us.
Amy S. Porter, HT (ASCP) QIHC
Investigative HistoPathology Laboratory - Supervisor
2201 Biomedical Physical Sciences Bldg. Rm #2133
East Lansing, MI 48824-3320
Phone: (517) 884-5026
Fax: (517) 432-1368
Email: port...@msu.edu
Web: www.humanpathology.msu.edu
----- Original Message -----
From: "Barone, Carol " <cbar...@nemours.org>
To: <histonet@lists.utsouthwestern.edu>
Sent: Thursday, February 18, 2010 1:23 PM
Subject: [Histonet] DRGs
Histonetter's...we received a "boat-load" of mouse DRGs that had been
prepared in histogel and are cutting...well..not so good.
We normally do DRGs from FS and get beautiful results.
We have used histogel before with other small sample and have never had
issues... not sure if it is the Histogel or DRG's (fixed in 10% NBF and
then transferred to 70% before placed into the histogel).is the issue..I
seem to remember that histogel requires formalin and wonder if the
transfer to 70% is causing our problem ...but, obviously there is not
much room for error with such tiny- tiny samples and they are already
process and in paraffin?
I am not quite sure how twe can improve the ones that came in histogel,
and were processed to paraffin a paraffin block....any idea's? any
experience? any anything? Thx- ASAP!
cbar...@nemours.org
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