Although what you say is true, it is worth noting that there are some antigens/antibodies more amenable to acid decal. I have experienced this myself several times recently and been burned by thinking EDTA will always give better IHC results. At least for murine bone marrow vasculature antigens, this appears to be the case for some antibodies. Unfortunately as with most things in histology, there is always exceptions to rules of thumb!
--- On Fri, 2/19/10, Rene J Buesa <rjbu...@yahoo.com> wrote: From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] EDTA To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu>, "Dorothy LWebb" <dorothy.l.w...@healthpartners.com> Date: Friday, February 19, 2010, 9:03 PM EDTA (which stands for Ethylene-diamino-tratacetic-acid) is a chelating agent and the method of choice to decalcify BM biopsies. The thing is that it should be used alone, and not combined with any acid, as you state (not even formic acid). By itself will produce an extremely gentle decalcification that will be completely suitable for IHC studies. René J. --- On Fri, 2/19/10, Webb, Dorothy L <dorothy.l.w...@healthpartners.com> wrote: From: Webb, Dorothy L <dorothy.l.w...@healthpartners.com> Subject: [Histonet] EDTA To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Date: Friday, February 19, 2010, 1:29 PM I am looking into the various decals on the market and have found one that in addition to formic acid, has EDTA in the mix. I have never worked with EDTA so would appre ciate any help in your comments on the use of EDTA in decalcification methods for bone marrow and routine specimens. Thank you ahead of time for your advice! Dorothy Webb, HT (ASCP) Regions Histology Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
