I used to sterilze my TBS by autoclaving it. For aliquots I used TBS with 0,1% sodium acide. I never had any problems with bacteria; my 10x TBS concentrate was fine for weeks.
----- Original Message ----- From: amosbro...@gmail.com To: histonet@lists.utsouthwestern.edu Date: 24.02.2010 17:59:45 Subject: [Histonet] Tris Buffer Preservative > Hi, > I have been making up my own tris buffer for immunohistochemistry. It has > been working swimmingly, with one exception. I have been doing some work on > Salmonella, and apparently the researcher has been seeing bacteria in her > immunofluorescent slides that she says are moving and seem to be viable. She > wanted to culture all my reagents. So the primary and secondary antibody > diluents (commercially purchased) came up clean, but the TBS ended up with a > pretty healthy growing colony. Now since I don't do IHC overnight in an > incubator, I don't think this is necessarily a catastrophy. (No one else has > noticed this) It does seem to warrant further investigation though. So for > the folks that make their own solutions up, what do you use as a > preservative for your buffers and how much do you use. I haven't seen > anything in any of the recipes I have found. I was thinking Sodium Azide, > but it is really hazardous, and Wikipedia says it is actually explosive ( > http://en.wikipedia.org/wiki/Sodium_azide). Biocare Medical's data sheet > says they use less than 0.25% procyclin. (thanks for not hiding the > ingredients Biocare, I love you for that!) Has anyone tried that? Any other > suggestions would be welcome. > > Thanks, > Amos > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet