Hi Kim, Try probe on plus slides by fisherbrand for frozen samples. We use it for sectioning frozen tendon.
Maria V. De Guzman| Histology Technician I Main 908.947.1100 Fax 908.947.1085 Direct 908.947.1482 Email mdeguz...@lifecell.com Mobile 732.688.1386 www.Lifecell.com LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Tuesday, March 02, 2010 10:05 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 76, Issue 2 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: CD31 in rats (Hernandez, Anna) 2. CD 31 on Pig (Reuel Cornelia) 3. Re: CD 31 on Pig (Alexandra Meinl) 4. IgG4 (Martha Ward) 5. RE: Histonet Digest, Vol 76, Issue 1 (Madary, Joseph) 6. Herpes Simplex Virus Types 1 & 2 for IHC (Sebree Linda A) 7. Job opening (kim.dona...@bhcpns.org) 8. Ab for Cartilage (Tamasi, Joseph) 9. HSV I & II (Sebree Linda A) 10. inquiry about Arthur Bolles Lee (LINDA MARGRAF) 11. Microwave processing and IHCs (Green JumpyOne) 12. RE: Microwave processing and IHCs (WILLIAM DESALVO) 13. Re: RE: [Histonet] CD31 in rats (TF) 14. RE: HSV I & II (Hoekert, W.E.J.) 15. RE: Microwave processing and IHCs (Mike Pence) 16. Re: Microwave processing and IHCs (Rene J Buesa) 17. RE: Microwave processing and IHCs (McMahon, Loralee A) 18. FW: [Histonet] Microwave processing and IHCs (Vickroy, Jim) 19. RE: inquiry about Arthur Bolles Lee (Horn, Hazel V) ---------------------------------------------------------------------- Message: 1 Date: Mon, 1 Mar 2010 13:01:03 -0500 From: "Hernandez, Anna" <anna.hernan...@crl.com> Subject: RE: [Histonet] CD31 in rats To: "Annette Featherstone" <a...@buffalo.edu>, <histonet@lists.utsouthwestern.edu> Message-ID: <63ec5e7c7346eb4ba09d6dc5651e061bea5...@ent-pr-xch-02.na01.crl.com> Content-Type: text/plain; charset="us-ascii" Hello Annette, We use Purified Mouse anti-Rat CD31 from BD Biosciences (Catalog# 555025). We use on frozen sections fixed with acetone for 10 minutes with the following steps: 1X Morphosave (Ventana) (15 min) 0.3% H202 quench(Sigma) (20 min) Avidin and Biotin block (Vector) (15 min each) Covance blocking reagent (25 min) Mouse anti-Rat CD31 (25 min) at 2.0 ug/mL Biotinylated Horse anti-Mouse IgG (Vector-BA-2001) (25 minutes) at 5.0 ug/mL Covance Labeling Reagent (25 min) Covance Dab (2 x 5 min) (Each step has PBS washes in between and is performed at RT) Thanks, Anna -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Annette Featherstone Sent: Monday, March 01, 2010 7:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD31 in rats Is anyone using CD31 antibody in rats, if so, what vendor and what protocol. thanks Annette Featherstone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 01 Mar 2010 12:10:13 -0600 From: "Reuel Cornelia" <reuel.corne...@tsrh.org> Subject: [Histonet] CD 31 on Pig To: <histonet@lists.utsouthwestern.edu> Message-ID: <4b8baea5020000c500071...@mail.tsrh.org> Content-Type: text/plain; charset=US-ASCII Is anyone using CD 31 on Pigs. Where do you purchase your antibody. Thank you. Reuel ------------------------------ Message: 3 Date: Mon, 1 Mar 2010 19:37:31 +0100 From: Alexandra Meinl <alexandra.me...@gmail.com> Subject: Re: [Histonet] CD 31 on Pig To: Reuel Cornelia <reuel.corne...@tsrh.org>, a...@buffalo.edu Cc: histonet@lists.utsouthwestern.edu Message-ID: <b7eff7a1003011037o3f785045l5040524c802f4...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 We use the Rabbit anti-PECAM-1 (sc-1506-R, Santa Cruz) 1:100 on paraffin sections (HIER with citrate buffer pH 6.0 required). It works on pig and rat tissue. Alexandra 2010/3/1 Reuel Cornelia <reuel.corne...@tsrh.org> > Is anyone using CD 31 on Pigs. Where do you purchase your antibody. Thank > you. > > Reuel > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ************************************************ Dr. Alexandra Meinl Ludwig Boltzmann Institute for Experimental and Clinical Traumatology Austrian Cluster for Tissue Regeneration Histology Donaueschingenstrasse 13 1200 Vienna - Austria Contact @ Bernhard Gottlieb University School of Dentistry, Waehringerstr. 25a, A-1090 Vienna tel: +43 1 4277 67026 fax: +43 1 4277 67019 email: alexandra.me...@trauma.lbg.ac.at ------------------------------ Message: 4 Date: Mon, 1 Mar 2010 13:56:17 -0500 From: "Martha Ward" <mw...@wfubmc.edu> Subject: [Histonet] IgG4 To: <histonet@lists.utsouthwestern.edu> Message-ID: <61135f0455d33347b5aae209b903a30433deb...@exchvs2.medctr.ad.wfubmc.edu> Content-Type: text/plain; charset="us-ascii" Hello all, I am looking for a lab that offers this antibody on ffpe tissues. One of my Pathologists is interested in it and has a surgical case she has been asked to stain for IgG4. Thanks in advance for your help! Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 ------------------------------ Message: 5 Date: Mon, 1 Mar 2010 14:02:01 -0500 From: "Madary, Joseph" <mada...@medimmune.com> Subject: [Histonet] RE: Histonet Digest, Vol 76, Issue 1 To: <histonet@lists.utsouthwestern.edu> Message-ID: <29a3cb81288e6f4ba2c9b3c8015a9a1301459...@md1ev002.medimmune.com> Content-Type: text/plain; charset="iso-8859-1" I recall may years ago using the alcohol burner to heat my forceps and I was startled when the ice I had my blox on broke away from the pan and made a loud sound in an otherwise very quiet lab. I knocked over the alcohol burner and started a major lab fire. I used the extinguisher and all was fine but it took a while to get it all out. The biggest fear was the waste containers(good reason to recycle. Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Mgr, OMW, Area 4, Lab 2438 301.398.4745(vm) 301.398.6360(lab) 301.398.9745(fax) -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Monday, March 01, 2010 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 76, Issue 1 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Fire in the lab (Cheryl) 2. Fw: [Histonet] frozen sections of cartilage (Kim Merriam) 3. Pathologist Assistant Jobs (Alisha Dynan) 4. RELIA Histology Careers Bulletin 03/01/10 Are you into Facebook? MySpace? Twitter? or Linkedin? (Pam Barker) 5. CD31 in rats (Annette Featherstone) ---------------------------------------------------------------------- Message: 1 Date: Sun, 28 Feb 2010 17:14:17 -0800 (PST) From: Cheryl <tkngfl...@yahoo.com> Subject: [Histonet] Fire in the lab To: histonet@lists.utsouthwestern.edu Message-ID: <595933.44936...@web50901.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Amazing that your tech had the presence of mind to do ALL of those things! ? Used to work with a couple of old school techs back in the days when folks smoked in the lab.? One tech would embed with a xylene soaked rag to wipe the plate and a cigarette hanging out of her mouth.? She also used an open bunsen burner to keep her forceps hot....cannot tell you how many times she set fire to that rag...used her coffee to put it out most times... ? As Louise says--in another time and dimension. ? Cheryl --- On Sun, 2/28/10, histonet-requ...@lists.utsouthwestern.edu <histonet-requ...@lists.utsouthwestern.edu> wrote: From: histonet-requ...@lists.utsouthwestern.edu <histonet-requ...@lists.utsouthwestern.edu> Subject: Histonet Digest, Vol 75, Issue 39 To: histonet@lists.utsouthwestern.edu Date: Sunday, February 28, 2010, 10:01 AM Send Histonet mailing list submissions to ??? histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ???1. Re: Fire in the lab (louise renton) ???2. RE: SPAM-LOW:? [Histonet] ruo antibodies (Patsy Ruegg) ???3. Fire in the lab (Jeffrey Silverman) ???4. RELIA Histology Careers Bulletin 2/28/10 Are you into ? ? ? Facebook? MySpace? Twitter? or Linkedin? (Pam Barker) ---------------------------------------------------------------------- Message: 1 Date: Sat, 27 Feb 2010 20:51:00 +0200 From: louise renton <louise.ren...@gmail.com> Subject: Re: [Histonet] Fire in the lab To: Histonet@lists.utsouthwestern.edu Message-ID: ??? <e483362e1002271051l711a19e9y45fc3bbf47142...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 hey, I once set my hair on fire in the lab...singed off my eyebrows and burnt my nostril hair. It took several days for the burnt hair smell to get out of my nose!!. This too was in a time long long ago in another time dimension On Sat, Feb 27, 2010 at 12:51 AM, Joe Nocito <jnoc...@satx.rr.com> wrote: > Once upon a time in a far away land, we used to boil our embedding molds in > boiling soapy water, over an open Bunsen burner, followed by an alcohol > rinse then air dry. One time the fire alarm was activated and we had to > evacuate the hospital. We were out there quit awhile. When we received the > all clear to go back into the hospital, I was the first one back in the lab > and the fire department was there, looking into our pot that had boiled out > and was smoking up the lab. This wasn't the cause of the first alarm, but it > did set off the second. > > Joe > ----- Original Message ----- From: "CHRISTIE GOWAN" <christiego...@msn.com> > > > To: <histonet@lists.utsouthwestern.edu> > Sent: Friday, February 26, 2010 8:20 AM > > Subject: [Histonet] Fire in the lab > > > > > >? Dear Histonet Friends, > > I just wanted to share an incident we recently had with an old paraffin > pot. One of my techs came in on Sunday to embed some tissues, went into the > processor room and smelled something burning. He noticed our old paraffin > pot had charred looking labels on the outside so he went over, opened the > lid and poof!!! the pot went up in flames. The thermostat had gone haywire > and heated the paraffin to flash point. Opening the lid gave it the oxygen > it needed to ignite. He triggered the alarm, made the appropriate call and > then put it out with an extinguisher. Of course it kept re-igniting because > he could not get behind it to pull the plug. The fire dept finally was able > to get it pulled out and unplugged. Needless to say the tech was shaken and > the room was a mess. I applaud his courage and am not sure I would have done > the same. There was enough xylene and alcohol on the 4 processors to cause > quite an explosion but everything else was in a flammable cabinet. I was > wondering if this type of thing had ever happened to anyone else?? Needless > to say, we have de-comissioned all old paraffin pots and will order only > those with over temp safety features. I guess I just wanted to remind > everyone that fires can happen in the lab and do probably more often than we > hear about. This was the first time for me and I have been in this business > for over 20 years. Take care and be safe. > > Christie Gowan HT (ASCP) >? ? ? _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ------------------------------ Message: 2 Date: Sat, 27 Feb 2010 12:47:01 -0700 From: "Patsy Ruegg" <pru...@ihctech.net> Subject: RE: SPAM-LOW:? [Histonet] ruo antibodies To: "'Vickroy, Jim'" <vickroy....@mhsil.com>, ??? <Histonet@lists.utsouthwestern.edu> Message-ID: <5e6e0ab0ed28430797b6300065c77...@prueggihctechlt> Content-Type: text/plain;??? charset="us-ascii" Yea we were just looking at this yesterday.? There is no question on the CAP check list about using RUO's anymore, but it is still in the discussion section with ASR's and it is stated as you mentioned to include a disclaimer and statement that you did try to find an IVD or ASR antibody.? Our question was about billing the patient for RUO use.? CAP does not really address billing issues like that, but CMS does as far as we could tell, and we have been told that if you bill for RUO's CMS could come back and deny payment of the claim and perhaps all bills from your lab. We were wondering if anyone is billing patients for RUO antibodies, but of course if you are you may not want to say so to bring attention to yourself. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Friday, February 26, 2010 10:03 AM To: Histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] ruo antibodies Our new CAP checklist does not mention the use of RUO antibodies anymore. This was under the question using ASR antibodies in the past.???I believe the requirement was that if we wanted to use an RUO antibody we had to have a disclaimer similar to the ASR disclaimer but we also had to have a statement stating that we had searched for an IVD or ASR antibody.? Does anyone know if this is still the practice or am I missing something????I do know of course than when using either an ASR or RUO antibody we have to establish and verify the performance.???Any thoughts about the RUO(s)? James Vickroy BS, HT(ASCP) Surgical? and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Sat, 27 Feb 2010 12:30:35 -0800 (PST) From: Jeffrey Silverman <pathmas...@yahoo.com> Subject: [Histonet] Fire in the lab To: histonet@lists.utsouthwestern.edu Message-ID: <442926.94178...@web111115.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Here's mine. Had a per diem tech who?didn't like the electric forceps warmer.? She worked over Saturdays alone and brought in an alcohol lamp to heat her forceps and then knocked it over spilling flaming alcohol all over the thermal and dispensing consoles. Then, even worse, she poured water all over both plugged- in electic appliances. We got away with just melted facade?face plates on both instruments. ? Jeff Silverman ------------------------------ Message: 4 Date: Sat, 27 Feb 2010 22:12:50 -0500 From: "Pam Barker" <rel...@earthlink.net> Subject: [Histonet] RELIA Histology Careers Bulletin 2/28/10 Are you ??? into??? Facebook? MySpace? Twitter? or Linkedin? To: "'Histonet'" <histonet@lists.utsouthwestern.edu> Message-ID: <e1nlzam-00088f...@elasmtp-spurfowl.atl.sa.earthlink.net> Content-Type: text/plain;??? charset="us-ascii" Hi Histonetters!! Facebook, Myspace, Linkedin, Twitter;? Have you joined the Social Networking Craze? If so then you know how much fun it can be reconnecting with old friends, making new ones.? If you do have an account on one of these networks I would love to connect with you.? Please shoot me back an e-mail with your id on the site or an invitation to connect/friend/follow and I will respond right away. If that was like a foreign language to you and you would like some help using any of these sites please let me know.? I would be happy to help you join in. I also wanted to tell you about my current job openings.? All of these jobs are full time permanent positions and my clients offer excellent compensation, benefits and relocation assistance.? Need help with your resume or following up after the interview or negotiating salary?? Give me a call or shoot me an e-mail.? I would be happy to help out.? This is a complimentary service of RELIA of course. Here is a list of my current open positions HISTOLOGY/PATHOLOGY? MANAGEMENT NY- Orange/Rockland County Histology Supervisor MA - Cape Cod Pathology Supervisor OR-Portland-Pathology Manager WA-Spokane-Histology Supervisor-Hospital CA - Central CA - Pathology Supervisor HISTOTECHS FL-Miami Histotechnologist needed for growing private lab GA-Grossing Histotechnologist Night Shift Great Shift diff MA-Boston - Immunohistochemistry Specialist MA-Boston - Histotechs day and evening shift NY-Orange/Rockland County Brand New Lab NYS license or Elig 2nd shift. CA-Los Angeles Histotechnologist afternoon shift PATHOLOGY/PATHOLOGIST'S ASSISTANTS NC - Charlotte PA grad from NAACLES program required FL- Miami Growing private lab MARKETING AND SALES Marketing Rep - Histo/Cyto Products Pacific NW Region CYTOLOGY PA-Pittsburgh Cytology Supervisor If you or any of your friends would like more information on any of the positions listed or help with a job search in another area please contact me.? I would be more than happy to assist you.? You can reach me at 866-607-3542 or? <blocked::mailto:rel...@earthlink.net> rel...@earthlink.net Remember it never hurts to look. Hope to hear from you soon. Thanks-Pam Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:? ? (407)353-5070 FAX:? ? (407)678-2788 Toll Free: (866)607-3542 e-mail:? <blocked::mailto:rel...@earthlink.net> rel...@earthlink.net <blocked::http://home.earthlink.net/~relia1> http://home.earthlink.net/~relia1 <blocked::http://www.myspace.com/pamatrelia> www.myspace.com/pamatrelia <blocked::http://www.twitter.com/pamatrelia> www.twitter.com/pamatrelia ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 75, Issue 39 **************************************** ------------------------------ Message: 2 Date: Mon, 1 Mar 2010 04:46:09 -0800 (PST) From: Kim Merriam <kmerriam2...@yahoo.com> Subject: Fw: [Histonet] frozen sections of cartilage To: Histonet <histonet@lists.utsouthwestern.edu> Message-ID: <836501.4247...@web50303.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Guys, I never received a response and I was?hoping that someone would have some tips.? We?are embedding and sectioning rat and mouse knee cartilage and I?am having trouble keeping it on the slides.? Even when I do my IHC by hand, the sections are still?lifting off.? Any suggestions? Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ----- Forwarded Message ---- From: Kim Merriam <kmerriam2...@yahoo.com> To: Histonet <histonet@lists.utsouthwestern.edu> Sent: Mon, February 22, 2010 2:37:58 PM Subject: [Histonet] frozen sections of cartilage Hi Everyone, Any tips for keeping frozen sections of cartilage from falling off the slides?during?IHC staining?? We should not have to do HIER, but they still fall of the slides quite easily. Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: 1 Mar 2010 10:11:11 -0500 From: Alisha Dynan <ali...@ka-recruiting.com> Subject: [Histonet] Pathologist Assistant Jobs To: histonet@lists.utsouthwestern.edu Message-ID: <1496304024.1267456271654.javamail.cfserv...@webserver53> Content-Type: text/plain; charset="utf-8" Dear Histonet Subscribers, I hope you are doing well. I am a recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Laboratory Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory positions across the country. Our clients typically assist with relocation expenses. One particular client I am working with is one of the top hospital systems in the country. This hospital in Tennessee is looking for a Pathologist Assistant for their Pathology Department. My client is looking for someone who is PA(ASCP) certified and has at least 5 years experience as a pathologist assistant. This position has a very competitive base salary, great benefits, and an unparalleled retirement package. My client will also assist with relocation expenses. We are also working on a number of other opportunities. See the titles below and let me know if you would like more details! Other Current Opportunities: NV - Pathologist's Assistant 2nd shift TN - Pathologist's Assistant 1st shift (must have Master's) Midwest - Pathologist's Assistant 1st shift Southern CA - Pathologist Assistant Supervisor If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Laboratory positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 ali...@ka-recruiting.com www.ka-recruiting.com ------------------------------ Message: 4 Date: Mon, 1 Mar 2010 10:28:38 -0500 From: "Pam Barker" <rel...@earthlink.net> Subject: [Histonet] RELIA Histology Careers Bulletin 03/01/10 Are you into Facebook? MySpace? Twitter? or Linkedin? To: "'Histonet'" <histonet@lists.utsouthwestern.edu> Message-ID: <e1nm7xz-0007hv...@elasmtp-spurfowl.atl.sa.earthlink.net> Content-Type: text/plain; charset="iso-8859-1" Hello Histonetters!! Facebook, Myspace, Linkedin, Twitter; Have you joined the Social Networking Craze? If so then you know how much fun it can be reconnecting with old friends, making new ones. If you do have an account on one of these networks I would love to connect with you. Please shoot me back an e-mail with your id on the site or an invitation to connect/friend/follow and I will respond right away. If that was like a foreign language to you and you would like some help using any of these sites please let me know. I would be happy to help you join in. I also wanted to tell you about my current job openings. All of these jobs are full time permanent positions and my clients offer excellent compensation, benefits and relocation assistance. Need help with your resume or following up after the interview or negotiating salary? Give me a call or shoot me an e-mail. I would be happy to help out. This is a complimentary service of RELIA of course. Here is a list of my current open positions HISTOLOGY/PATHOLOGY MANAGEMENT MA-Cape Cod Pathology Supervisor OR-Portland-Pathology Manager WA-Spokane-Histology Supervisor-Hospital CA-Central CA-Pathology Supervisor HISTOTECHS FL-Miami Histotechnologist needed for growing private lab GA-Grossing Histotechnologist Night Shift Great Shift diff MA-Boston - Immunohistochemistry Specialist MA-Boston - Histotechs day and evening shift NY-Orange/Rockland County Brand New Lab NYS license or Elig 2nd shift. CA-Los Angeles Histotechnologist afternoon shift PATHOLOGY/PATHOLOGIST'S ASSISTANTS NC - Charlotte PA grad from NAACLES program required FL- Miami Growing private lab MARKETING AND SALES Marketing Rep - Histo/Cyto Products Pacific NW Region CYTOLOGY PA-Pittsburgh Cytology Supervisor If you or any of your friends would like more information on any of the positions listed or help with a job search in another area please contact me. I would be more than happy to assist you. You can reach me at 866-607-3542 or <mailto:rel...@earthlink.net> rel...@earthlink.net Remember it never hurts to look... Hope to hear from you soon. Thanks-Pam Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll Free: (866)607-3542 e-mail: <mailto:rel...@earthlink.net> rel...@earthlink.net <http://home.earthlink.net/~relia1> http://home.earthlink.net/~relia1 <http://www.myspace.com/pamatrelia> www.myspace.com/pamatrelia <http://www.twitter.com/pamatrelia> www.twitter.com/pamatrelia ------------------------------ Message: 5 Date: Mon, 1 Mar 2010 10:44:51 -0500 From: "Annette Featherstone" <a...@buffalo.edu> Subject: [Histonet] CD31 in rats To: <histonet@lists.utsouthwestern.edu> Message-ID: <000001cab956$2215a3d0$6640eb...@edu> Content-Type: text/plain; charset="us-ascii" Is anyone using CD31 antibody in rats, if so, what vendor and what protocol. thanks Annette Featherstone ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 76, Issue 1 *************************************** To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. ------------------------------ Message: 6 Date: Mon, 1 Mar 2010 13:10:34 -0600 From: "Sebree Linda A" <lseb...@uwhealth.org> Subject: [Histonet] Herpes Simplex Virus Types 1 & 2 for IHC To: "Histonet" <histo...@pathology.swmed.edu> Message-ID: <8c023b4ab999614ba4791baeb26e27381bf...@uwhc-mail01.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="us-ascii" Good afternoon, Does anyone have a "nice" HSV I & II concentrate antibody for IHC on FFPE that they use. Biocare recently discontinued their concentrate and we don't do enough of these to warrant buying a predilute. We'd like a cocktail of the 2 types. Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 ------------------------------ Message: 7 Date: Mon, 1 Mar 2010 13:39:54 -0600 From: kim.dona...@bhcpns.org Subject: [Histonet] Job opening To: histonet@lists.utsouthwestern.edu Message-ID: <of51867145.edf35f9b-on862576d9.006a62bf-862576d9.006c0...@bhcpns.org> Content-Type: text/plain; charset="US-ASCII" Hi Histo World, We have a Histologist position available. Seeking someone self motivated, personable and team oriented! Anyone interested please email me. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. ------------------------------ Message: 8 Date: Mon, 1 Mar 2010 15:00:26 -0500 From: "Tamasi, Joseph" <joseph.tam...@bms.com> Subject: [Histonet] Ab for Cartilage To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <845938d0fdd6b54bad926f60ff10a6a803e12a2...@ushpwbmsmmp009.one.ads.bms.com> Content-Type: text/plain; charset="us-ascii" Dear Histonetters, One of my colleagues is interested in demonstrating a marker for terminal chondrocyte differentiation in rat bone. It would be very helpful for her to know what antibodies others have used successfully for IHC. Thank you in advance for your responses. Joe Tamasi Senior Research Scientist Bristol-Myers Squibb 311 Pennington-Rocky-Hill Road Pennington, NJ 08534 609-818-3288 joseph.tam...@bms.com ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ------------------------------ Message: 9 Date: Mon, 1 Mar 2010 14:24:20 -0600 From: "Sebree Linda A" <lseb...@uwhealth.org> Subject: [Histonet] HSV I & II To: "Histonet" <histo...@pathology.swmed.edu> Message-ID: <8c023b4ab999614ba4791baeb26e27381bf...@uwhc-mail01.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="us-ascii" Thanks to all who responded to my inquiry about HSV I&II however I'd like to find it in a cocktail. I have since learned that manufacturers can no longer premix ASR antibodies to sell . I guess I'll have to find out if are docs need both or can make do with one. Thanks again, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 ------------------------------ Message: 10 Date: Mon, 01 Mar 2010 16:14:15 -0600 From: "LINDA MARGRAF" <linda.marg...@childrens.com> Subject: [Histonet] inquiry about Arthur Bolles Lee To: <histonet@lists.utsouthwestern.edu> Message-ID: <4b8be7d7.f783.00d...@childrens.com> Content-Type: text/plain; charset="us-ascii" Dear Histonetters I got this request which I presume was intended for the Histonet list. Does anyone have information on this gentleman I can pass along? Dear Linda, I would appreciate it you could seek or make an inquiry for me. I am looking for an obituary and other biographical information about the histotechnologist Arthur Bolles Lee (1849 - 1927). Sincerely, Frederick H. Kasten Johnson City, TN Thanks... Linda M Histonet administrator </pre> <span style="color: rgb(0, 160, 0); font-weight: bold;">Please consider the environment before printing this e-mail</span><br /> <br /> <span style="font-size: 8pt;">This e-mail, facsimile, or letter and any files or attachments transmitted with it contains<br /> information that is confidential and privileged. This information is intended only for the use of the <br /> individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further <br /> disclosures are prohibited without proper authorization. If you are not the intended recipient, any <br /> disclosure, copying, printing, or use of this information is strictly prohibited and possibly a <br /> violation of federal or state law and regulations. If you have received this information in error, <br /> please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at <br /> priv...@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all <br /> applicable privileges related to this information.</span><br /> <br /> </html> ------------------------------ Message: 11 Date: Mon, 1 Mar 2010 17:15:23 -0800 From: Green JumpyOne <greenjumpy...@hotmail.com> Subject: [Histonet] Microwave processing and IHCs To: <histonet@lists.utsouthwestern.edu> Message-ID: <bay107-w17e2a9630b5b1116543001ab...@phx.gbl> Content-Type: text/plain; charset="iso-8859-1" Does anyone here have any experience with using microwave tissue processing and then performing IHC stains on those same tissues? We have new microwave processors and are trying to determine if we will need to get all new controls for our IHCs that have been microwave processed. If yes, we will need to do validations on the controls, but I am wondering if the MW will have any discernible effect on the reactivity of the tissue. thanks for any help! Michelle _________________________________________________________________ Hotmail: Free, trusted and rich email service. http://clk.atdmt.com/GBL/go/201469228/direct/01/ ------------------------------ Message: 12 Date: Mon, 1 Mar 2010 19:23:00 -0700 From: WILLIAM DESALVO <wdesalvo....@hotmail.com> Subject: RE: [Histonet] Microwave processing and IHCs To: <greenjumpy...@hotmail.com>, histonet <histonet@lists.utsouthwestern.edu> Message-ID: <blu103-w140460961f87f42a3c389791...@phx.gbl> Content-Type: text/plain; charset="Windows-1252" I have used the Sakura Xpress for 5+ years and have had great success w/ all staining, w/ little to no changes. The Xpress does not use Xylene and that was a change from our conventional processing and reagents, so we did have to collect new control tissues. I say that anytime you change the process, you MUST collect new control tissue and you will ned to document. When CAP comes to visit and they see a MW processor, you will need to show them your validation and control tissue documentation. If you have the possibility of using both conventional or Microwave processd tissue, then make a modified sausage block to include both types of processing protocols, so that work can flow through the lab smoothly. William DeSalvo, B.S., HTL(ASCP) > From: greenjumpy...@hotmail.com > To: histonet@lists.utsouthwestern.edu > Date: Mon, 1 Mar 2010 17:15:23 -0800 > Subject: [Histonet] Microwave processing and IHCs > > > Does anyone here have any experience with using microwave tissue processing > and then performing IHC stains on those same tissues? > > We have new microwave processors and are trying to determine if we will need > to get all new controls for our IHCs that have been microwave processed. If > yes, we will need to do validations on the controls, but I am wondering if > the MW will have any discernible effect on the reactivity of the tissue. > > thanks for any help! > > Michelle > > _________________________________________________________________ > Hotmail: Free, trusted and rich email service. > http://clk.atdmt.com/GBL/go/201469228/direct/01/_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail: Trusted email with Microsoft?s powerful SPAM protection. http://clk.atdmt.com/GBL/go/201469226/direct/01/ ------------------------------ Message: 13 Date: Tue, 2 Mar 2010 11:32:29 +0800 From: "TF" <ti...@foxmail.com> Subject: Re: RE: [Histonet] CD31 in rats To: "Hernandez, Anna" <anna.hernan...@crl.com>, "Annette Featherstone" <a...@buffalo.edu>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <201003021132284371...@foxmail.com> Content-Type: text/plain; charset="gb2312" Hi, we use Abcam mouse-anti CD31 also acetone fixed frozen sections. pretty well. 2010-03-02 TF ???????? Hernandez, Anna ?????????? 2010-03-02 09:50:38 ???????? Annette Featherstone; histonet@lists.utsouthwestern.edu ?????? ?????? RE: [Histonet] CD31 in rats Hello Annette, We use Purified Mouse anti-Rat CD31 from BD Biosciences (Catalog# 555025). We use on frozen sections fixed with acetone for 10 minutes with the following steps: 1X Morphosave (Ventana) (15 min) 0.3% H202 quench(Sigma) (20 min) Avidin and Biotin block (Vector) (15 min each) Covance blocking reagent (25 min) Mouse anti-Rat CD31 (25 min) at 2.0 ug/mL Biotinylated Horse anti-Mouse IgG (Vector-BA-2001) (25 minutes) at 5.0 ug/mL Covance Labeling Reagent (25 min) Covance Dab (2 x 5 min) (Each step has PBS washes in between and is performed at RT) Thanks, Anna -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Annette Featherstone Sent: Monday, March 01, 2010 7:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD31 in rats Is anyone using CD31 antibody in rats, if so, what vendor and what protocol. thanks Annette Featherstone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Tue, 2 Mar 2010 10:37:02 +0100 From: "Hoekert, W.E.J." <w.e.j.hoek...@olvg.nl> Subject: RE: [Histonet] HSV I & II To: "Sebree Linda A" <lseb...@uwhealth.org>, "Histonet" <histo...@pathology.swmed.edu> Message-ID: <1190cb05c44b13409483514729c2fc360c0...@pait42.olvg.nl> Content-Type: text/plain; charset="iso-8859-1" Too bad that all these people did not respond to the entire group. We are using the one from Immunologic (polyclonal, ILP4114-C1). Is this one also not available anymore? Willem Hoekert OLVG, Netherlands ________________________________ Van: histonet-boun...@lists.utsouthwestern.edu namens Sebree Linda A Verzonden: ma 1-3-2010 21:24 Aan: Histonet Onderwerp: [Histonet] HSV I & II Thanks to all who responded to my inquiry about HSV I&II however I'd like to find it in a cocktail. I have since learned that manufacturers can no longer premix ASR antibodies to sell . I guess I'll have to find out if are docs need both or can make do with one. Thanks again, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. ------------------------------ Message: 15 Date: Tue, 2 Mar 2010 08:03:59 -0600 From: "Mike Pence" <mpe...@grhs.net> Subject: RE: [Histonet] Microwave processing and IHCs To: "Green JumpyOne" <greenjumpy...@hotmail.com>, <histonet@lists.utsouthwestern.edu> Message-ID: <661949901a768e4f9cc16d8af8f2838c017a3...@is-e2k3.grhs.net> Content-Type: text/plain; charset="us-ascii" Your control tissue needs to be processed the same as your patient samples. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Green JumpyOne Sent: Monday, March 01, 2010 7:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave processing and IHCs Does anyone here have any experience with using microwave tissue processing and then performing IHC stains on those same tissues? We have new microwave processors and are trying to determine if we will need to get all new controls for our IHCs that have been microwave processed. If yes, we will need to do validations on the controls, but I am wondering if the MW will have any discernible effect on the reactivity of the tissue. thanks for any help! Michelle _________________________________________________________________ Hotmail: Free, trusted and rich email service. http://clk.atdmt.com/GBL/go/201469228/direct/01/________________________ _______________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Tue, 2 Mar 2010 06:03:58 -0800 (PST) From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] Microwave processing and IHCs To: histonet@lists.utsouthwestern.edu, Green JumpyOne <greenjumpy...@hotmail.com> Message-ID: <458340.86147...@web65704.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Michelle: The key in MW tissue processing is to accelerate the process and it is very likely that the quality of the tissues will be the same as if processed in a more "traditional" way, at least that is the claim and the objective. Having said that this does not mean that you don't have to demonstrate it with a validation process. You should always have controls processed in the very same way as the patients' tissues so in theory you have to obtain a new set of controls processed with the your MW tissue processor. The complication develops if you have also a "conventional" tissue processor and then you will need two sets of controls. Ideally all your cases should be processed with a single method. Ren? J. --- On Mon, 3/1/10, Green JumpyOne <greenjumpy...@hotmail.com> wrote: From: Green JumpyOne <greenjumpy...@hotmail.com> Subject: [Histonet] Microwave processing and IHCs To: histonet@lists.utsouthwestern.edu Date: Monday, March 1, 2010, 8:15 PM Does anyone here have any experience with using microwave tissue processing and then performing IHC stains on those same tissues? We have new microwave processors and are trying to determine if we will need to get all new controls for our IHCs that have been microwave processed.? If yes, we will need to do validations on the controls, but I am wondering if the MW will have any discernible effect on the reactivity of the tissue. thanks for any help! Michelle ??? ???????? ?????? ??? ? _________________________________________________________________ Hotmail: Free, trusted and rich email service. http://clk.atdmt.com/GBL/go/201469228/direct/01/_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Tue, 2 Mar 2010 09:15:11 -0500 From: "McMahon, Loralee A" <loralee_mcma...@urmc.rochester.edu> Subject: RE: [Histonet] Microwave processing and IHCs To: Mike Pence <mpe...@grhs.net>, Green JumpyOne <greenjumpy...@hotmail.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <c27aa2a01cef31469813089e226f582e63759...@urmcms7.urmc-sh.rochester.edu> Content-Type: text/plain; charset="us-ascii" In my experience I have not seen any change in the MW tissue versus the routine processing. What we have tried to do since we have so many different antibodies and control tissues is create mini sausage blocks with one piece of MW tissue and one piece of non MW tissue. Because we were not always sure what tissue were microwaved and what tissues were not. For example one small piece of tonsil microwaved and one non microwaved embedded in the same block. That way no matter what your patient tissue sample has gone through you have all the bases covered. It takes a long time to set up but will save you time in the end. If you run your tissues when you validate the microwave you'll have half of the process finished. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence [mpe...@grhs.net] Sent: Tuesday, March 02, 2010 9:03 AM To: Green JumpyOne; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microwave processing and IHCs Your control tissue needs to be processed the same as your patient samples. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Green JumpyOne Sent: Monday, March 01, 2010 7:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave processing and IHCs Does anyone here have any experience with using microwave tissue processing and then performing IHC stains on those same tissues? We have new microwave processors and are trying to determine if we will need to get all new controls for our IHCs that have been microwave processed. If yes, we will need to do validations on the controls, but I am wondering if the MW will have any discernible effect on the reactivity of the tissue. thanks for any help! Michelle _________________________________________________________________ Hotmail: Free, trusted and rich email service. http://clk.atdmt.com/GBL/go/201469228/direct/01/________________________ _______________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Tue, 2 Mar 2010 08:37:49 -0600 From: "Vickroy, Jim" <vickroy....@mhsil.com> Subject: FW: [Histonet] Microwave processing and IHCs To: "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Message-ID: <24a4826e8ef0964d86bc5317306f58a54257908...@mmc-mail.ad.mhsil.com> Content-Type: text/plain; charset="us-ascii" See below James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 -----Original Message----- From: Vickroy, Jim Sent: Tuesday, March 02, 2010 8:16 AM To: 'Mike Pence' Subject: RE: [Histonet] Microwave processing and IHCs I understand and agree in principle. However let me ask this: If a validation study shows that there is no difference between conventional tissue processing and microwave tissue processing in your IHC protocols then why can't you then use controls that are processed either by conventional or microwave. If we can't then are we using controls that have been decaled for tissues that have to be decaled and so forth. I have always believed if you can validate there is no appreciable difference in methods than we can use the same controls. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Tuesday, March 02, 2010 8:04 AM To: Green JumpyOne; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microwave processing and IHCs Your control tissue needs to be processed the same as your patient samples. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Green JumpyOne Sent: Monday, March 01, 2010 7:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave processing and IHCs Does anyone here have any experience with using microwave tissue processing and then performing IHC stains on those same tissues? We have new microwave processors and are trying to determine if we will need to get all new controls for our IHCs that have been microwave processed. If yes, we will need to do validations on the controls, but I am wondering if the MW will have any discernible effect on the reactivity of the tissue. thanks for any help! Michelle _________________________________________________________________ Hotmail: Free, trusted and rich email service. http://clk.atdmt.com/GBL/go/201469228/direct/01/________________________ _______________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ------------------------------ Message: 19 Date: Tue, 2 Mar 2010 08:49:50 -0600 From: "Horn, Hazel V" <hor...@archildrens.org> Subject: RE: [Histonet] inquiry about Arthur Bolles Lee To: "LINDA MARGRAF" <linda.marg...@childrens.com>, <histonet@lists.utsouthwestern.edu> Message-ID: <9ae8aa9e1f644b4aa6c155fb6fd51c6317d83...@email.archildrens.org> Content-Type: text/plain; charset="us-ascii" If you plug his name into google several links appear.. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of LINDA MARGRAF Sent: Monday, March 01, 2010 4:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] inquiry about Arthur Bolles Lee Dear Histonetters I got this request which I presume was intended for the Histonet list. Does anyone have information on this gentleman I can pass along? Dear Linda, I would appreciate it you could seek or make an inquiry for me. I am looking for an obituary and other biographical information about the histotechnologist Arthur Bolles Lee (1849 - 1927). Sincerely, Frederick H. Kasten Johnson City, TN Thanks... Linda M Histonet administrator </pre> <span style="color: rgb(0, 160, 0); font-weight: bold;">Please consider the environment before printing this e-mail</span><br /> <br /> <span style="font-size: 8pt;">This e-mail, facsimile, or letter and any files or attachments transmitted with it contains<br /> information that is confidential and privileged. This information is intended only for the use of the <br /> individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further <br /> disclosures are prohibited without proper authorization. If you are not the intended recipient, any <br /> disclosure, copying, printing, or use of this information is strictly prohibited and possibly a <br /> violation of federal or state law and regulations. If you have received this information in error, <br /> please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at <br /> priv...@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all <br /> applicable privileges related to this information.</span><br /> <br /> </html> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 76, Issue 2 *************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet