My brief experience with paraffin and fluorescence (DAPI and EGFP) is that
the paraffin embedding process destroys it.
Regards,
Merced
--On Tuesday, March 02, 2010 3:25 PM -0600 Fabrice gankam
<gan...@googlemail.com> wrote:
Hi,
Just wondering if anyone has use hydroethinide to detect the free
radicals (ROS) in the CNS or anyother tissue of rats after 4% formallin
fixation and parrafin embedding.
I wanted to used the hydroethidine on paraffin sections.
I wonder if the fluorescence is lost by fixation (12hrs in 4% PAF) and
paraffin embedding plus deparaffination.
All the papers I reviewed used hydroethidine on frozen section but our
facility does not have vibratome or cryostat.
Please help
Please Help.
Fabrice
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Merced M Leiker
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Cardiovascular Medicine
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State University of New York at Buffalo
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