We too use the Universal negative from Biocare, and it has passed through 2 CAP inspections so no problem on that front.
Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gagnon, Eric Sent: Thursday, March 04, 2010 10:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Negative controls Hi Anita, We are currently examining the negative control issue in our laboratory. We also have Ventanas. We currently use a normal goat serum as our "non-immune" serum. We are considering changing our SOP's to use the Ventana Neg Control Rabbit and Neg Control Mouse in dispensers. Linda's suggestion of Biocare's Universal Negative Control Serum is intriguing. It would seem regressive to go back to the days of delineating mouse/rabbit antisera, using separate dispensers for each, and a universal negative seems to be the way to go, especially when using universal DAB kits. Using such a reagent would mean it could be applied to each case's negative control slide. Linda or others, would such a negative control serum protocol include your most aggressive pretreatment? Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet