Hi: I'm not sure if this helps but Botanists have long used tert-butanol (not N-butanol) to embed tissue in paraffin. This classic protocol was popularized by D. A. Johansen in the mid 1930's and involves transition from water to alcohol to t-butanol (I can supply more detailed info if you need it). Once in 100% t-butanol tissue is transitioned to light paraffin oil. The final stage involves filling some large shell vials 1/3 full with paraffin and letting them harden. Tissue plus paraffin oil is added to the vials (volume of paraffin oil + tissue should be be approximately equal to the amount of paraffin). Vials are now placed in an oven for paraffin to melt and infiltration to begin. Besides doing enough changes of paraffin to infiltrate the tissue and remove all the paraffin oil that's about it. From there its the old embed, section and enjoy!
Hope this helps - Mike Folsom Rio Grande Biological mwfol...@rgbio.com On Mon, 2010-03-08 at 09:00 -0600, Krueger, Todd wrote: > Does anyone have a procedure for processing with N-butyl Alcohol. We > need to find a procedure w/o xylene. > Thanks > > Todd Krueger > > HTL(ASCP)CM > > Boston Scientific > > 2 Scimed Place, P121 > > Osseo, MN 55311 > > Phone: 763-694-5709 > > Fax: 763-694-5505 > > e-mail: todd.krue...@bsci.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet