CBG Biotech offers hydrometers. Regards, Beth Austin CBG Biotech 1-800-941-9484 ext 200
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 76, Issue 11 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Helicobacter Pylori antibody (Chakib Boussahmain) 2. RE: Helicobacter Pylori antibody (McMahon, Loralee A) 3. RE: Helicobacter Pylori antibody (Sebree Linda A) 4. Tissue Tek (ricky hachy) 5. RE: Helicobacter Pylori antibody (Cynthia Pyse) 6. Re: Helicobacter Pylori antibody (Kim Tournear) 7. Re: Eosin too pink (Kim Tournear) 8. hydrometer (Vickroy, Jim) 9. Re: hydrometer (dkb...@chs.net) 10. Region II mtg addiiton and up-date: (Barone, Carol ) 11. p16 (Knutson, Deanne) 12. Re: Butyl alcohol (Rene J Buesa) 13. Masson Trichrome, Alligator Heart Tissue, colors turning out purple (cscam...@uci.edu) 14. Need help with FISH staining protocol on frozen tissue sections (Shen, Jinhui) 15. Re: p16 (Dana Settembre) 16. RE: p16 (Cynthia Pyse) 17. Masson Trichrome, Alligator Heart Tissue, colors turning out purple (Durden, Kelley) 18. Microwave Tissue Processor Protocols (Green JumpyOne) 19. HistoDay (Amy Johnson) 20. Histology Professionals Day (Breeden, Sara) 21. Re: Histology Professionals Day (Nita Searcy) 22. Happy HPD to our "Unsung Heroes" (Alyssa Peterson) 23. Re: Need help with FISH staining protocol on frozen tissue sections (Merced M Leiker) 24. RE: Histology Professionals Day (Beckham, Sharon) 25. RE: HistoDay (Marsh, Nannette) 26. RE: HistoDay (Marsh, Nannette) 27. Histo Professionals Day (Breeden, Sara) ---------------------------------------------------------------------- Message: 1 Date: Tue, 9 Mar 2010 10:45:54 -0800 (PST) From: Chakib Boussahmain <chak_...@yahoo.com> Subject: [Histonet] Helicobacter Pylori antibody To: histonet@lists.utsouthwestern.edu Message-ID: <591938.93716...@web58105.mail.re3.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hey Histonet, Is anyone using Helicobacter pylori antibody? if so, can you tell me where did you get it? and the protocol? Any input will appreciated Thank you so much. Chakib HTL(ASCP) MIT-DCM ------------------------------ Message: 2 Date: Tue, 9 Mar 2010 13:52:36 -0500 From: "McMahon, Loralee A" <loralee_mcma...@urmc.rochester.edu> Subject: RE: [Histonet] Helicobacter Pylori antibody To: Chakib Boussahmain <chak_...@yahoo.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <c27aa2a01cef31469813089e226f582e63759...@urmcms7.urmc-sh.rochester.edu> Content-Type: text/plain; charset="us-ascii" I have ordered mine from Cell Marque. We use it at 1/50 dilution with a 30 minute incubation at RT on the Dako Autostainers. Works great. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Chakib Boussahmain [chak_...@yahoo.com] Sent: Tuesday, March 09, 2010 1:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Helicobacter Pylori antibody Hey Histonet, Is anyone using Helicobacter pylori antibody? if so, can you tell me where did you get it? and the protocol? Any input will appreciated Thank you so much. Chakib HTL(ASCP) MIT-DCM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Tue, 9 Mar 2010 12:58:14 -0600 From: "Sebree Linda A" <lseb...@uwhealth.org> Subject: RE: [Histonet] Helicobacter Pylori antibody To: "Chakib Boussahmain" <chak_...@yahoo.com>, <histonet@lists.utsouthwestern.edu> Message-ID: <8c023b4ab999614ba4791baeb26e27381bf...@uwhc-mail01.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="iso-8859-1" Hi Chakib, We use Ventana's (manufactured by Cell Marque) H. Pylori on their instruments. The protocol is specific to the instruments but involves a routine HIER step and Avidin/Biotin blocking. The antibody itself is ready-to-use but is available from Cell Marque as a concentrate as well. The protocol produces a very clean stain; essential since these bugs are so small. Hope this helps, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Chakib Boussahmain Sent: Tuesday, March 09, 2010 12:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Helicobacter Pylori antibody Hey Histonet, Is anyone using Helicobacter pylori antibody? if so, can you tell me where did you get it? and the protocol? Any input will appreciated Thank you so much. Chakib HTL(ASCP) MIT-DCM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Tue, 9 Mar 2010 19:00:49 +0000 From: ricky hachy <elc...@hotmail.com> Subject: [Histonet] Tissue Tek To: Histonet <histonet@lists.utsouthwestern.edu> Message-ID: <snt114-w552e106567733d196e0811cf...@phx.gbl> Content-Type: text/plain; charset="iso-8859-1" I need the 2 parafin pots for a Tissue Tek/Sakura tissue processor working or not . Anyone has them ? Regards Ricky _________________________________________________________________ Your E-mail and More On-the-Go. Get Windows Live Hotmail Free. http://clk.atdmt.com/GBL/go/201469229/direct/01/ ------------------------------ Message: 5 Date: Tue, 9 Mar 2010 14:16:08 -0500 From: "Cynthia Pyse" <cp...@x-celllab.com> Subject: RE: [Histonet] Helicobacter Pylori antibody To: "'Chakib Boussahmain'" <chak_...@yahoo.com>, <histonet@lists.utsouthwestern.edu> Message-ID: <000001cabfbc$f93ecdc0$ebbc69...@com> Content-Type: text/plain; charset="iso-8859-1" We purchase it from Biocare. Protocol: pretreatment Diva (Biocare) 20 minutes water bath 20 minutes cool down. Detection system Mach 4 (Biocare) 10 minutes H2O2, 10 minute primary antibody dilution 1:100, 5 minutes mouse probe, 12 minutes polymer, 10 minutes DAB(Dako), 5 minutes hematoxylin(Dako). Works great. Cindy -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Chakib Boussahmain Sent: Tuesday, March 09, 2010 1:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Helicobacter Pylori antibody Hey Histonet, Is anyone using Helicobacter pylori antibody? if so, can you tell me where did you get it? and the protocol? Any input will appreciated Thank you so much. Chakib HTL(ASCP) MIT-DCM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Tue, 9 Mar 2010 11:52:54 -0800 (PST) From: Kim Tournear <kimtourn...@yahoo.com> Subject: Re: [Histonet] Helicobacter Pylori antibody To: Chakib Boussahmain <chak_...@yahoo.com>, Histonet <histonet@lists.utsouthwestern.edu> Message-ID: <544545.25352...@web54204.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 We use Cell Marque's Polyclonal H. Pylori (CMC435) at a 1:100 dilution on the Ventana BenchMark for 32 minutes, Protease 1 for 12 minutes. ~Kim Tournear ~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks!!!! ________________________________ From: Chakib Boussahmain <chak_...@yahoo.com> To: histonet@lists.utsouthwestern.edu Sent: Tue, March 9, 2010 11:45:54 AM Subject: [Histonet] Helicobacter Pylori antibody Hey Histonet, Is anyone using Helicobacter pylori antibody? if so, can you tell me where did you get it? and the protocol? Any input will appreciated Thank you so much. Chakib HTL(ASCP) MIT-DCM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 9 Mar 2010 12:00:43 -0800 (PST) From: Kim Tournear <kimtourn...@yahoo.com> Subject: Re: [Histonet] Eosin too pink To: "Smith, Lesley" <lesley.sm...@ramsayhealth.co.uk>, Histonet <histonet@lists.utsouthwestern.edu> Message-ID: <382091.74830...@web54203.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 We add 100mL of 100% alcohol to 150mL of Eosin to tone it down each day. ~Kim Tournear ~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks!!!! ________________________________ From: "Smith, Lesley" <lesley.sm...@ramsayhealth.co.uk> To: histonet@lists.utsouthwestern.edu Sent: Tue, March 9, 2010 7:51:46 AM Subject: [Histonet] Eosin too pink Our Pathologists have, over the last few weeks, complained that the eosin is too pink. We have not altered our procedures or reagents in any way. It seems to be particularly bad in endoscopic biopsies. Thanks Lesley Smith Senior Biomedical Scientist Cellular Pathology The Yorkshire Clinic Bradford Road Bingley BD16 1TW Switchboard: +44 1274 550600 ext 3348 Direct Dial: +44 1274 550800 http: www.ramsayhealth.co.uk <http://www.ramsayhealth.co.uk/> ************************************************ Disclaimer and Confidentiality Note Everything in this e-mail and any attachments relating to the official business of Ramsay Health Care UK Operations Limited (Ramsay) or any of its subsidiary or associated companies is proprietary. It is confidential, legally privileged and protected by law. Ramsay does not endorse any of the content. Views and opinions are those of the sender unless clearly stated as being that of Ramsay. The person addressed in the e-mail is the sole authorised recipient. Please notify the sender immediately if it has unintentionally reached you and do not read, disclose or use the content in any way. Ramsay can not ensure that the integrity of this communication has been maintained nor that it is free of errors, virus, interception or interference. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Tue, 9 Mar 2010 14:08:17 -0600 From: "Vickroy, Jim" <vickroy....@mhsil.com> Subject: [Histonet] hydrometer To: "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Message-ID: <24a4826e8ef0964d86bc5317306f58a54257bc3...@mmc-mail.ad.mhsil.com> Content-Type: text/plain; charset="us-ascii" Anybody have any idea where to purchase a simple hydrometer for checking the water content in alcohols? I found one at TBS but do not know if it will fit our purposes since it says use with a specific recycler. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ------------------------------ Message: 9 Date: Tue, 9 Mar 2010 15:12:05 -0500 From: dkb...@chs.net Subject: Re: [Histonet] hydrometer To: "Vickroy, Jim" <vickroy....@mhsil.com> Cc: "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu>, histonet-boun...@lists.utsouthwestern.edu Message-ID: <of34202695.26373c83-on852576e1.006eea59-852576e1.006ef...@chs.net> Content-Type: text/plain; charset="US-ASCII" We ordered ours from Fisher Scientific. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 10 Date: Tue, 9 Mar 2010 17:02:30 -0500 From: "Barone, Carol " <cbar...@nemours.org> Subject: [Histonet] Region II mtg addiiton and up-date: To: <histonet@lists.utsouthwestern.edu> Message-ID: <37e4bac017f57141af64faa5aeb04ce8033a7...@wlmmsx01.nemours.org> Content-Type: text/plain; charset="us-ascii" Histonetter's, Region II up-date: Save the date for the 2010 Region II Symposium! June 10-12, 2010. Clarion Hotel, Altlantic City, N.J ...Come for a day... or come for the weekend. Excellent Speakers, new workshops, vendor fair and of course...there is always the beach. ..(for studying, of course!). Stay tuned to the histonet, for more information regarding specific seminars and workshops. More to come... Information on registration will be posted on the histonet in April. So keep up with the histonet...for lots of great networking.... and info on the Region II Symposium See you there. Carol Barone - Region II Director...Oh!...and keep networking! It is the best educational tool of all! ------------------------------ Message: 11 Date: Tue, 9 Mar 2010 16:27:30 -0600 From: "Knutson, Deanne" <dknut...@primecare.org> Subject: [Histonet] p16 To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Message-ID: <1e0e2b14c709174b8ac2be0ae7f768338fe967c...@exchange2k7.staprimecare.org> Content-Type: text/plain; charset="us-ascii" Hello fellow Histonetters, Does anyone run the antibody p16? If so, where do you purchase it from and maybe you can share your protocol with me? Thank you in advance. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center (701)-530-6730 dknut...@primecare.org<mailto:dknut...@primecare.org> ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. ------------------------------ Message: 12 Date: Tue, 9 Mar 2010 14:43:54 -0800 (PST) From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] Butyl alcohol To: ToddKrueger <todd.krue...@bsci.com>, Michael Folsom <mwfol...@rgbio.com> Cc: histonet@lists.utsouthwestern.edu Message-ID: <169396.76443...@web65709.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 As an additional note: paraffin oil = mineral oil. Reni J. --- On Tue, 3/9/10, Michael Folsom <mwfol...@rgbio.com> wrote: From: Michael Folsom <mwfol...@rgbio.com> Subject: Re: [Histonet] Butyl alcohol To: "Krueger, Todd" <todd.krue...@bsci.com> Cc: histonet@lists.utsouthwestern.edu Date: Tuesday, March 9, 2010, 11:17 AM Hi: I'm not sure if this helps but Botanists have long used tert-butanol (not N-butanol) to embed tissue in paraffin. This classic protocol was popularized by D. A. Johansen in the mid 1930's and involves transition from water to alcohol to t-butanol (I can supply more detailed info if you need it). Once in 100% t-butanol tissue is transitioned to light paraffin oil. The final stage involves filling some large shell vials 1/3 full with paraffin and letting them harden. Tissue plus paraffin oil is added to the vials (volume of paraffin oil + tissue should be be approximately equal to the amount of paraffin). Vials are now placed in an oven for paraffin to melt and infiltration to begin. Besides doing enough changes of paraffin to infiltrate the tissue and remove all the paraffin oil that's about it. From there its the old embed, section and enjoy! Hope this helps - Mike Folsom Rio Grande Biological mwfol...@rgbio.com On Mon, 2010-03-08 at 09:00 -0600, Krueger, Todd wrote: > Does anyone have a procedure for processing with N-butyl Alcohol. We > need to find a procedure w/o xylene. > Thanks > > Todd Krueger > > HTL(ASCP)CM > > Boston Scientific > > 2 Scimed Place, P121 > > Osseo, MN 55311 > > Phone: 763-694-5709 > > Fax: 763-694-5505 > > e-mail: todd.krue...@bsci.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Tue, 9 Mar 2010 17:06:21 -0800 From: cscam...@uci.edu Subject: [Histonet] Masson Trichrome, Alligator Heart Tissue, colors turning out purple To: histonet@lists.utsouthwestern.edu Message-ID: <88928dfe83ca47dbf12638e73258f4a1.squir...@webmail.uci.edu> Content-Type: text/plain;charset=iso-8859-1 To Histonet: This is my first time mailing the histonet community. Coincidentally, this is my first venture into histology. My lab is conducting research on alligator heart tissue. At the moment, I am attempting to stain my slides using Masson Trichrome to differentiate muscle from connective tissue. Results have not been optimal. The slides turn out to be more purple than I had anticipated (comparing to pictures from journals). The red can be told apart from the teal, but the colors are not bright. Here is the procedure I am using: 1. Deparaffinize and hydrate to water 2. Iron Alum, 30 min 3. Wash in Dis. Water, 5 min 4. Hematoxylin stain, 30 min 5. Wash in Dis. Water, 5 min 6. Differentiate in saturated aq. picric acid, 1 min 7. Wash in dis. water, 10 min 8. Stain in acid fuschin, 5 min 9. Rinse in water 10. Stain in Ponceau de Xylidene, 5 min 11. Rinse in water 12. Differentiate in Phosphomolybdic acid, 5 min 13. Fast Green, 2 min 14. Differentiate in 1% aq. acetic acid, 5 min 15. Dehydrate 16. xylene (two washes, 3 min each) I would greatly appreciate any advice on how to modify my procedure to better stain the cardiac tissue. Or should I be using a different trichrome method better suited to this particular tissue? Thank you! -Colin ------------------------------ Message: 14 Date: Wed, 10 Mar 2010 00:44:08 -0600 From: "Shen, Jinhui" <jin...@uta.edu> Subject: [Histonet] Need help with FISH staining protocol on frozen tissue sections To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <f1cf91345a31fc4795bcde7836d0eab54f46f66...@mavmail2.uta.edu> Content-Type: text/plain; charset="us-ascii" Hi everyone, Has anyone done FISH staining on mice tissue frozen sections for Y chromosome? I need help with a successful protocol. Recently I started to do FISH staining on mice liver frozen sections for chromosome Y, but got no positive results even on male samples yet. The probe I used was IDMF1057 Green label Chr Y probe from ID Labs Inc. (www.idlabs.com). They have a protocol online probably for cultured cells (http://www.idlabs.com/product/datasheets/IDMF1057.pdf?PHPSESSID=b39f16507cf 76b69495166c651f0b52c) which I haven't tried yet. I followed the following protocol for frozen sections: 1. Fix sections in 4% PFA at 4C for 10 mins, then wash in PBS. 2. Treat the sections with 0.01% Pepsin, 37C 10min, wash in PBS. 3. Dehydrate the slides using 70% Ethanol (2minsX2), 90% Ethanol (2mins X2), 100% Ethanol (5 mins X1).Then Age the slides at 65C for one hour. 4. Mix the probe and hybridization buffer. Denature the probe by incubate it at 65C for 10 mins, then hold it at 37C for 30 - 60 mins. 5. Denature the sections by incubate them in denaturing solution (formamide:2XSSC, 70:30) at 75C for 2 mins. 6. Quench the slides in 4C 70% Ethanol for 4 mins. Dehydrate the slides as step 3. 7. Apply the probe and hybridize at 37C overnight. 8. Immerse the slides in 1XSSC for 5 mins to remove the cover slips. 9. Wash slides in stringency solution (formamide:2XSSC, 50:50) at 45C, 5 mins X2. 10. Wash slides in 1XSSC at 45C, 5minsX2. 11. Wash slides in detergent wash solution at 45C, 5minX1. 12. Rinse slides in PBS. 13. Air dry for a couple of minutes and counterstain with DAPI. 14. Mount the slides and observe the results or keep in 4C for late check. Are there any problems with this procedure? Please help me to check, or if you could recommend a working protocol you had used, I would really appreciate. Thanks, Jinhui ------------------------------ Message: 15 Date: Wed, 10 Mar 2010 08:06:57 -0500 From: "Dana Settembre" <sette...@umdnj.edu> Subject: Re: [Histonet] p16 To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu>, "Deanne Knutson" <dknut...@primecare.org> Message-ID: <sb975329....@smtpnpc.umdnj.edu> Content-Type: text/plain; charset=US-ASCII Hi Deanne, My understanding is that you must get p16 from MTM Laboratories. They have some sort of monopoly right now. I still have some leftover from Cell Marque and when I run low or the antibody is about to expire I must also go to MTM. I currently use the Cell Marque p16 Ready To Use by pretreating with Dako's TRS for 40min in steamer, and detect with labelled polymer. Dako's Envision + Mouse kit. It works nicely. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> "Knutson, Deanne" <dknut...@primecare.org> 03/09/10 5:27 PM >>> Hello fellow Histonetters, Does anyone run the antibody p16? If so, where do you purchase it from and maybe you can share your protocol with me? Thank you in advance. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center (701)-530-6730 dknut...@primecare.org<mailto:dknut...@primecare.org> ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Wed, 10 Mar 2010 08:25:25 -0500 From: "Cynthia Pyse" <cp...@x-celllab.com> Subject: RE: [Histonet] p16 To: "'Knutson, Deanne'" <dknut...@primecare.org>, <histonet@lists.utsouthwestern.edu> Message-ID: <000001cac055$24c65010$6e52f0...@com> Content-Type: text/plain; charset="US-ASCII" We purchase our p16 through MTM laboratories reference # 9517. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Knutson, Deanne Sent: Tuesday, March 09, 2010 5:28 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] p16 Hello fellow Histonetters, Does anyone run the antibody p16? If so, where do you purchase it from and maybe you can share your protocol with me? Thank you in advance. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center (701)-530-6730 dknut...@primecare.org<mailto:dknut...@primecare.org> ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Wed, 10 Mar 2010 09:15:19 -0500 From: "Durden, Kelley" <kelleydur...@pathology.ufl.edu> Subject: [Histonet] Masson Trichrome, Alligator Heart Tissue, colors turning out purple To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <92e6b93e0a3d544c87ddde33e7608aae591db...@hsc-cms01.ad.ufl.edu> Content-Type: text/plain; charset="us-ascii" I've been using the Masson Trichrome kit from Richard Allan since 2001 and I've always gotten really great results. It is a little more expensive than using home made reagents but the results are very consistent. Kelley ------------------------------ Message: 18 Date: Wed, 10 Mar 2010 06:44:18 -0800 From: Green JumpyOne <greenjumpy...@hotmail.com> Subject: [Histonet] Microwave Tissue Processor Protocols To: <histonet@lists.utsouthwestern.edu> Message-ID: <bay107-w37dd5a5d44a2566d4154f5ab...@phx.gbl> Content-Type: text/plain; charset="iso-8859-1" I am working on the validation of our microwave processors and have noticed that the tissues are a bit dry. Would anyone be willing to share their protocol timings for their fixation, ethanol, isopropyl and wax impregnation steps? We are using the Histos-5 microwaves from Milestone and are utilizing the 2mm (for the smalls) and the 3mm (for the larger specimens) default program settings. thanks for any help! Michelle ps, my apologies to the board for someone spamming the board using a spoof of my email address. :o( _________________________________________________________________ Your E-mail and More On-the-Go. Get Windows Live Hotmail Free. http://clk.atdmt.com/GBL/go/201469229/direct/01/ ------------------------------ Message: 19 Date: Wed, 10 Mar 2010 08:54:11 -0600 From: "Amy Johnson" <ajohn...@aipathology.com> Subject: [Histonet] HistoDay To: <histonet@lists.utsouthwestern.edu> Message-ID: <704247d5a09d004c9e6b115138d1703a1c4...@hpserv001.aipathology.local> Content-Type: text/plain; charset="us-ascii" Happy Histology Professionals Day!!!!!! When I was going to school at Marshfield one of the Pathologists told me that without histotechs his work would be much harder. Thank you Dr. Krawicz for appreciating the job we do!!! ------------------------------ Message: 20 Date: Wed, 10 Mar 2010 08:02:17 -0700 From: "Breeden, Sara" <sbree...@nmda.nmsu.edu> Subject: [Histonet] Histology Professionals Day To: "histonet" <histonet@lists.utsouthwestern.edu> Message-ID: <4d14f0fc9316dd41972d5f03c070908b02e46...@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="US-ASCII" If it weren't for a med-tech-turned-pathologist that took me up on my interest in becoming trained in histology (1967), I would not have just marked my 41st year as a histologist. Back then, you just had to have OJT and study independently for your written and practical tests. I had no idea what I was getting into but it was one of the absolute best choices I ever made. To pretend to quote some baseball player, "Histology been berry, berry good to me"! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ------------------------------ Message: 21 Date: Wed, 10 Mar 2010 09:07:17 -0600 From: "Nita Searcy" <nsea...@swmail.sw.org> Subject: Re: [Histonet] Histology Professionals Day To: "histonet" <histonet@lists.utsouthwestern.edu>, "Sara Breeden" <sbree...@nmda.nmsu.edu> Message-ID: <4b976145.5d38.00e...@swmail.sw.org> Content-Type: text/plain; charset="us-ascii" I too "stumbled" into the profession in 1967 and share Sara's sentiments. I sent the following to our senior staff as well as the supervisory group of our laboratory: Today is the first annual National Histotechnology day and is being celebrated by the histology staff by a morning breakfast ( in order that the entire staff can participate) and the wearing of special t-shirts. In spite of almost total anonymity to most of the general public, as well as a considerable amount of health care professionals, these highly skilled individuals play a vital role in healthcare. Wish them congratulations. Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsea...@swmail.sw.org 254-724-2438 >>> "Breeden, Sara" <sbree...@nmda.nmsu.edu> 3/10/2010 9:02 AM >>> If it weren't for a med-tech-turned-pathologist that took me up on my interest in becoming trained in histology (1967), I would not have just marked my 41st year as a histologist. Back then, you just had to have OJT and study independently for your written and practical tests. I had no idea what I was getting into but it was one of the absolute best choices I ever made. To pretend to quote some baseball player, "Histology been berry, berry good to me"! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:nsea...@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:nsea...@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD ------------------------------ Message: 22 Date: Wed, 10 Mar 2010 10:09:18 -0500 From: Alyssa Peterson <aly...@alliedsearchpartners.com> Subject: [Histonet] Happy HPD to our "Unsung Heroes" To: histonet@lists.utsouthwestern.edu Message-ID: <bbc6db3a1003100709i53003bf5q8e2eb2fc73ef2...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Allied Search Partners wants to wish you a Happy Histology Professionals Day! Histology professionals are the unsung heroes behind the laboratory doors. The patient may not know you but what you all do is the absolute best for them because what you do affects their lives and their loved ones lives. I want to thank each and every one of you for doing what you do behind closed doors away from the surgeons who see the patients and actually get to remove the problem. Thank you for being the Unspoken Heroes!! Please contact us today to inquire about Histology jobs in your area. Alyssa Peterson Director of Recruitment Allied Search Partners aly...@alliedsearchpartners.com WWW.ALLIEDSEARCHPARTNERS.COM <http://www.alliedsearchpartners.com/> ------------------------------ Message: 23 Date: Wed, 10 Mar 2010 10:13:35 -0500 From: Merced M Leiker <lei...@buffalo.edu> Subject: Re: [Histonet] Need help with FISH staining protocol on frozen tissue sections To: "Shen, Jinhui" <jin...@uta.edu>, histonet@lists.utsouthwestern.edu Message-ID: <cfaea1255b1583d39cf3e...@cdywxp1931.ad.med.buffalo.edu> Content-Type: text/plain; charset=us-ascii; format=flowed I have been using IDLabs porcine Y chromosome green label probe on frozen pig tissue with good results most of the time. You say you denature the probe and then hold it at 37oC...I would think that would allow the probe to reanneal to itself since you also hybridize later on at that temp...try following the IDLabs protocol where they say to co-denature the probe and the slide. I do this for my frozen sections and it works. Regards, Merced --On Wednesday, March 10, 2010 12:44 AM -0600 "Shen, Jinhui" <jin...@uta.edu> wrote: > Hi everyone, > > Has anyone done FISH staining on mice tissue frozen sections for Y > chromosome? I need help with a successful protocol. > > Recently I started to do FISH staining on mice liver frozen sections for > chromosome Y, but got no positive results even on male samples yet. The > probe I used was IDMF1057 Green label Chr Y probe from ID Labs Inc. > (www.idlabs.com). They have a protocol online probably for cultured cells > (http://www.idlabs.com/product/datasheets/IDMF1057.pdf?PHPSESSID=b39f1650 > 7cf76b69495166c651f0b52c) which I haven't tried yet. I followed the > following protocol for frozen sections: > > 1. Fix sections in 4% PFA at 4C for 10 mins, then wash in PBS. > > 2. Treat the sections with 0.01% Pepsin, 37C 10min, wash in PBS. > > 3. Dehydrate the slides using 70% Ethanol (2minsX2), 90% Ethanol (2mins > X2), 100% Ethanol (5 mins X1).Then Age the slides at 65C for one hour. > > 4. Mix the probe and hybridization buffer. Denature the probe by incubate > it at 65C for 10 mins, then hold it at 37C for 30 - 60 mins. > > 5. Denature the sections by incubate them in denaturing solution > (formamide:2XSSC, 70:30) at 75C for 2 mins. > > 6. Quench the slides in 4C 70% Ethanol for 4 mins. Dehydrate the slides > as step 3. > > 7. Apply the probe and hybridize at 37C overnight. > > 8. Immerse the slides in 1XSSC for 5 mins to remove the cover slips. > > 9. Wash slides in stringency solution (formamide:2XSSC, 50:50) at 45C, 5 > mins X2. > > 10. Wash slides in 1XSSC at 45C, 5minsX2. > > 11. Wash slides in detergent wash solution at 45C, 5minX1. > > 12. Rinse slides in PBS. > > 13. Air dry for a couple of minutes and counterstain with DAPI. > > 14. Mount the slides and observe the results or keep in 4C for late check. > > Are there any problems with this procedure? Please help me to check, or > if you could recommend a working protocol you had used, I would really > appreciate. > > Thanks, > > Jinhui > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ------------------------------ Message: 24 Date: Wed, 10 Mar 2010 09:13:46 -0600 From: "Beckham, Sharon" <s...@stowers.org> Subject: [Histonet] RE: Histology Professionals Day To: "'Breeden, Sara'" <sbree...@nmda.nmsu.edu>, histonet <histonet@lists.utsouthwestern.edu> Message-ID: <bd62cbac4395b94096109020651be2ec12ff121...@exchmb-02.stowers-institute.org> Content-Type: text/plain; charset="us-ascii" Sally, I have pretty much the same story. Back in 1970 I applied for a pathology secretary position and the pathologist asked me if I could sew, which meant he wondered if I could do intricate work with my hands. In my spare time, I trained in histology and took my registry in 1973. It was the best decision I ever made to take him up on his offer. I love, love, love being a histologist and hope that my health remains good so I can do it for many more years to come. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, March 10, 2010 9:02 AM To: histonet Subject: [Histonet] Histology Professionals Day If it weren't for a med-tech-turned-pathologist that took me up on my interest in becoming trained in histology (1967), I would not have just marked my 41st year as a histologist. Back then, you just had to have OJT and study independently for your written and practical tests. I had no idea what I was getting into but it was one of the absolute best choices I ever made. To pretend to quote some baseball player, "Histology been berry, berry good to me"! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 25 Date: Wed, 10 Mar 2010 09:13:45 -0600 From: "Marsh, Nannette" <n...@stowers.org> Subject: [Histonet] RE: HistoDay To: 'Amy Johnson' <ajohn...@aipathology.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <bd62cbac4395b94096109020651be2ec12fefe2...@exchmb-02.stowers-institute.org> Content-Type: text/plain; charset="us-ascii" Here's a quote that we have posted on our Histology event board "Ah, the Professional Hostologist! Walk in awe when you meet one....because there are only a few people who can produce something as beautiful and useful as a slide." by Drs. William Osler and F.E. Mohs----just about says it all, doesn't it? -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Johnson Sent: Wednesday, March 10, 2010 8:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HistoDay Happy Histology Professionals Day!!!!!! When I was going to school at Marshfield one of the Pathologists told me that without histotechs his work would be much harder. Thank you Dr. Krawicz for appreciating the job we do!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 26 Date: Wed, 10 Mar 2010 09:18:54 -0600 From: "Marsh, Nannette" <n...@stowers.org> Subject: [Histonet] RE: HistoDay To: 'Amy Johnson' <ajohn...@aipathology.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <bd62cbac4395b94096109020651be2ec12fefe2...@exchmb-02.stowers-institute.org> Content-Type: text/plain; charset="us-ascii" Okay--so I don't proof read very well--that was supposed to be 'histologist'--not hostologist in my last post--although there are days when hostility abounds in clinical pathology!!!!! -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Johnson Sent: Wednesday, March 10, 2010 8:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HistoDay Happy Histology Professionals Day!!!!!! When I was going to school at Marshfield one of the Pathologists told me that without histotechs his work would be much harder. Thank you Dr. Krawicz for appreciating the job we do!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 27 Date: Wed, 10 Mar 2010 08:21:42 -0700 From: "Breeden, Sara" <sbree...@nmda.nmsu.edu> Subject: [Histonet] Histo Professionals Day To: "histonet" <histonet@lists.utsouthwestern.edu> Message-ID: <4d14f0fc9316dd41972d5f03c070908b02e46...@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="US-ASCII" Perhaps what each of us ought to do is write a quick line naming the one person that got you involved in histology and how they did that. I'd be willing to gather them and pass them on to NSH so they could post it at the Seattle meeting. If you'd like to do this, send the email to nmhi...@comcast.net and I'll collect. Just an idea... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 76, Issue 11 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet