Dear Julie, Thanks for the information! I saw some papers about that staining on mice tissues, such as http://www.pnas.org/content/104/10/4030.abstract . I have been talking with Ms Leiker at Univ. of Bufflo, sorry I forgot to click "reply to all" but "reply". She gave me some good suggestions and I am going to try the staining again next week, will let you know if it works out or if I need more help. Thanks!
Regards, Jinhui ________________________________________ From: J C [jcb...@gmail.com] Sent: Thursday, March 11, 2010 5:36 AM To: Shen, Jinhui Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Need help with FISH staining protocol on Dear Jinhui! We tried for a long time to perform SRY FISH for rats, paraffin, frosen nothinhg worked. I know other people that did not suceeded on other animals, I do not know anybody that could do it exept of somebody in German, that worked with special plasmid for rat. There are not repeated works in pubmed. I doubt that this method works good for rats or mices, but Cumbio has a kit, maybe it will work. Regards, Julie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
