The perma red is for HRP or alk phos. Depends on your detection polymer.
Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: Patsy Ruegg [pru...@ihctech.net] Sent: Saturday, March 13, 2010 11:02 AM To: McMahon, Loralee A; 'Gareth Blaeuer Davis'; histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: RE: [Histonet] Red Chromogen for MITF antibody Is the Perma Red for hrp? If you switch to an alk.phos detection system you could use fast red which is much redder than AEC. Dako sells ap/red detection systems, but I prefer to use Vector Red for AP. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A Sent: Friday, March 12, 2010 6:23 AM To: Gareth Blaeuer Davis; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: RE: [Histonet] Red Chromogen for MITF antibody We use Diagnostic Biosystems Perma Red with the Dako system. www.dbiosys.com Works very well Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gareth Blaeuer Davis [gareth.da...@hotmail.com] Sent: Thursday, March 11, 2010 7:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Red Chromogen for MITF antibody Hi, Does anyone do MITF immunostains with a Red Chromogen. We are using a Dako autostainer, and in the past have used AEC chromogen on MITF. Our pathologist doesn't like it, because it doesn't get red enough. We would like something we could use with current protocols on the Dako, but can't find anything. Any suggestions? Gareth Blaeuer Davis, HT, BS. Pathology Associates of St. Thomas Nashville, Tn 37205 615-298-4100 _________________________________________________________________ Hotmail: Free, trusted and rich email service. http://clk.atdmt.com/GBL/go/201469228/direct/01/____________________________ ___________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet