Hi, I processed small porcine spinal cord once (diameter ~3-5mm). My pathologist did not mention any abnormalities, so I guess our standard protocol for every type of tissue was fine.
for 1h each: NBF 70% isopropanol ( = 2-propanol) 80% isopropanol 95% isopropanol 100% isopropanol 100% isopropanol 100% isopropanol xylene xylene paraffine paraffine If nothing else helps, maybe you want to give that a try with slightly shorter times in the alcoholic solutions, as your specimen seems to be smaller in size. > Dear all, > I'm quite new in morphological analysis and Histology. > In my l=ab we are processing rat spinal cord to perform luxol fast > blue staining. > First we fix the spinal cord (only lumbar portion) in 4% > parafolmaldehyde (4h) =y immesion, then the samples are embedded in > paraffin using a authomatic Leica ASP300 machine. When observed, the > white matter of the spinal cord is full of holes and myelin seems to > be absent. > I guess the problem was related to the processing steps=erformed by > the machine, in particular the Xylol step. > Is there someone who can help me sharing his/her experience on spinal > cord process=ng protocol? > Any suggestion will be highly appreciated. > Regards > -- > Dott.ssa Elisa Ballarini,PhD student > Dipartimento di Neuroscie=ze e Tecnologie Biomediche > Università degli Studi Milano-Bicocca > Via C=dore 48-20052,Monza,MB > e.ballari...@campus.unimib.it > Tel. 02-64488119 > Fax. 02-64488253 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Am 15.03.2010 15:05, schrieb Elisa Ballarini: > Dear all, > I'm quite new in morphological analysis and Histology. > In my l=ab we are processing rat spinal cord to perform luxol fast > blue staining. > First we fix the spinal cord (only lumbar portion) in 4% > parafolmaldehyde (4h) =y immesion, then the samples are embedded in > paraffin using a authomatic Leica ASP300 machine. When observed, the > white matter of the spinal cord is full of holes and myelin seems to > be absent. > I guess the problem was related to the processing steps=erformed by > the machine, in particular the Xylol step. > Is there someone who can help me sharing his/her experience on spinal > cord process=ng protocol? > Any suggestion will be highly appreciated. > Regards > -- > Dott.ssa Elisa Ballarini,PhD student > Dipartimento di Neuroscie=ze e Tecnologie Biomediche > Università degli Studi Milano-Bicocca > Via C=dore 48-20052,Monza,MB > e.ballari...@campus.unimib.it > Tel. 02-64488119 > Fax. 02-64488253 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet