Dear Friends,
I am having a problem in IF stains on Renal Bx.In direct
immunofloresence on the frozen sections does not have disered contrast and many
of the cases its falsely negative.
Can you people tell me the possible mistake which may cause these kind of
effects on the IF stain.
I dont see any gross mistakes on the Frozen sections after sectioning.All the
temperature and other things are fine on the cryostate, but my pathologist
feels that there may be any problem in section.will that have any effects on
the IF stains..
Aazathraj.
Technical Officer.
Apollo Hospitals
India.
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