Hi Alison, Do you have a problem with all type of tissue ( Liver, Lung, Lymph Nodes, Trephine ... ) or just Trephine specimens. ?
If you only have problems with Retic on BMT, if you use Formic Formaldehyde Decal fluid, see if EDTA make a difference. If you experience issue with all type of tissue. The ammoniacal silver may be at fault, make a new batch and test it with liver or reactive Lymph Node hopefully it will work. In my lab we use the Gordon & Sweet Retic. Staining protocol copy can be found in any copy of Bancroft & Stevens or the newer version Bancroft & Gamble, and Bancroft & Cook if you don'g have access to any of these book you can download the staining protocol GORDON & SWEET'S RETIC<http://library.med.utah.edu/WebPath/HISTHTML/MANUALS/RETIC.PDF> Hope this help. Malika Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond Street Hospital London WC1N 3JH United Kingdom Tel: +44 20 7405 9200 ext 5475 Fax: +44 20 7829 7875 ben...@gosh.nhs.uk On Tue, Mar 23, 2010 at 8:35 PM, Scott, Allison D < allison_sc...@hchd.tmc.edu> wrote: > Hello to all in histoland. We are having a problem with our reticulin > stain. It is not showing a real delination of the reticulin fibers. > They are there but it tends to fade off. We do the brown and hopps > stain. Any help would be appreciated. > > Allison Scott HT(ASCP) > Histology Supervisor > LBJ Hospital > 5656 Kelley > Houston, Texas 77026 > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify the > sender by return e-mail and delete this e-mail and any attachments from > your computer system. > > To the extent the information in this e-mail and any attachments contain > protected health information as defined by the Health Insurance Portability > and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and > 164; or Chapter 181, Texas Health and Safety Code, it is confidential > and/or > privileged. This e-mail may also be confidential and/or privileged under > Texas law. The e-mail is for the use of only the individual or entity > named > above. If you are not the intended recipient, or any authorized > representative of the intended recipient, you are hereby notified that any > review, dissemination or copying of this e-mail and its attachments is > strictly prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- " Smile .... it confuses people " _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet