The pH of the stain may be too high, it should be 2.5-2.6 so check it
with a pH meter.
If the pH is fine you/your vendor may have a bad batch of dye since
under normal conditions the stain is difficult to wash out.
Make sure the dye is certified by the Biological Stain Commission. If
there is no certification tag the dye is not certified.
Geoff
Laurie Elmgren wrote:
Hi Everyone,
Lately, we have been having a problem with our Alcian Blue. It is fine
up to the washing step. Once it hits the water, the blue fades to
unacceptable. We have tried reducing the water rinse after the blue to
just a quick rinse in d-H20, and it is still poor. The vendor replaced
the kit, and it worked fine the first time, then two weeks later, the
same problem arose. Three different techs have tried the stain three
times. Any suggestions?
Laurie Elmgren
Histology Supervisor
Sunrise Medical Labs
240 Motor Pkwy
Hauppauge, NY 11788
(631)435-1515x1108
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intended recipient of this message you must not disseminate, copy or
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**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcaul...@umdnj.edu
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