Atoska Gentry asked: hello, if any of you have acetone fixation incorporated into you frozen section H& E staining protocol will you please advise me on adjustments necessary for routine staining protocol. Also, out of curiosity please enlighten me on the purpose for post sectioning acetone fixation on tissue samples initially fixed in 95% ETOH/ Acetic Acid? Your prompt replies will be greatly appreciated. ~ Atoska
Several thoughts come to mind. Acetone fixation is usually used only for preserving antigenicity on fresh frozen tissue samples. For slides needing H&E I would use a formalin fixative prior to the H&E procedure for fresh frozen tissues. Acetone fixed H&E stained cryosections are uuuuuuugly. You should not be doing frozen sections on tissues initially fixed in alcohol. Alcohol is an anti-freeze and you will not get good freezing/sectioning of the samples. You can section unfixed samples and then fix after mounting on glass slides in the Alcohol/acetic acid fix. Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
