Katie, Rinse the frozen OCT surrounded tissue in Hanks or similar cell culture fluid. Two to three times should be enough to remove the OCT
Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Katie Crosby Sent: Monday, 29 March 2010 11:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Removing tissues from OCT for molecular analysis Hello, Can someone suggest a means of removing tissues from OCT for subsequent molecular analysis? I did not find anything in the archives describing this exactly. Thanks, Katie Katie Crosby Immunohistochemistry Cell Signaling Technology 3 Trask Lane Danvers, MA 01923 Phone: 978-867-2352 Fax: 978-867-2400 WEB Site: www.cellsignal.com Toll Free: 1-877-616-CELL (877-616-2355) 1-877-678-TECH (877-678-8324) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet