Do you bake your slides? How old are your cut sections that you are testing?
We have found that long baking of the sections before staining? Like overnight at 60C causes the staining intensity to decrease. And if the sections are older than a month the staining intensity decreases as well. Just a thought. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick [b-freder...@northwestern.edu] Sent: Wednesday, March 31, 2010 3:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DAKO Her2 antibody Anyone out there noticing problems with the Her2 antibody for Dako? Seems real strong and deteriorating quickly. We order it in lots, so you can imagine... Seems like every run is different. Same stainer, same titre , same tech. Tissue is from all over the country and world so we cannot control fixation etc. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
