I just wanted to know if anybody who are working with Pig bone tissue fix in 10%NBF and decalcified in 14% EDTA, citrate buffer (dako) antigen retrieval pH7.0 and ph 9.0 by steaming for 20 minutes, what is their IHC detection kit for mouse monoclonal and rabbit polyclonal antibodies. I am using a Powervision kit from Immunovision, now Leica and I have a lot of background staining even with my negative stain. Please help me remedy the background stain.Thank you.
Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
