Jeff, Thermo Scientific (Formally NeoMarkers) CD5 (Clone SP19): Catalogue# RM-9119-S.
Concentration of 1:75 diluted w/Dako diluent. Antigen Retrieval: PH6.0 Citrate Target Retrieval (DAKO) for 35 min. @ 99 degrees C, followed by a 20 min. room temp. cool down. 35 minute primary antibody application. 30 minute Envision+ polyclonal (DAKO) secondary antibody detection. 7 minute DAB+ (DAKO). Counter-stain as you like. Results on this antibody are excellent. Best of Luck, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Howery, Jeffrey [jhowe...@yrmc.org] Sent: Friday, April 02, 2010 4:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD5 Can anyone share thier protocol for CD5. We use the Dako platform. Thanks Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet