On Assignment Healthcare is currently looking for a Histo Tech for a contract assignment here in the Bay Area.
This assignment would start ASAP and will be for an indefinite period of time as it is to covering for a leave of absence. Hours are: Monday through Friday, 5:30am to 2:00pm. For additional information please contact me via email. Erik Dokken Market Leader - Northwest Region www.oahealthcare.com -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Monday, April 05, 2010 10:05 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 77, Issue 5 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Ab Validation (Dana Settembre) 2. Histobath (Matthew Mincer) 3. Happy Easter! (mohamed abd el razik) 4. Re: Histobath (Malika Benatti) 5. Histobath part 2 (Matthew Mincer) 6. Re: Histobath part 2 (Lynette Pavelich) 7. Re: Histobath (Malika Benatti) 8. RE: 72644.18148...@web111105.mail.gq1.yahoo.com (Mahoney,Janice A) 9. RE: RCC (Bernice Frederick) 10. RE: CD5 (McMahon, Loralee A) 11. Choosing Carousel Style Tissue Processors (Parsons, Catherine) 12. RE: CD5 (Dawson, Glen) ---------------------------------------------------------------------- Message: 1 Date: Mon, 05 Apr 2010 06:30:51 -0400 From: "Dana Settembre" <sette...@umdnj.edu> Subject: Re: [Histonet] Ab Validation To: "Laurie Colbert" <laurie.colb...@huntingtonhospital.com>, <histonet@lists.utsouthwestern.edu> Message-ID: <sbb983a4....@smtpnpc.umdnj.edu> Content-Type: text/plain; charset=US-ASCII We have started saving these since the "NEW" question came up in June of 2009. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Laurie Colbert <laurie.colb...@huntingtonhospital.com> 04/02/10 11:19 AM >>> When you validate a new antibody or a new antibody lot, do you save the slides or just the paperwork (validation report from pathologist) for future inspection purposes? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 05 Apr 2010 07:37:56 -0500 From: Matthew Mincer <m...@techoneweb.com> Subject: [Histonet] Histobath To: histonet@lists.utsouthwestern.edu Message-ID: <4bb9d9a4.6020...@techoneweb.com> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Does anyone know the "normal" operating temperature of the Thermo Histobath? A customer has asked me to repair theirs and I want to make sure I get the temp correct. Thanks Matt -- Matthew Mincer Tech One Biomedical Service 159 N Marion Street PMB163 Oak Park, IL 60301 office (708) 383-6040 X 10 cell (708) 822-3738 ------------------------------ Message: 3 Date: Mon, 5 Apr 2010 05:46:25 -0700 (PDT) From: mohamed abd el razik <k8...@yahoo.com> Subject: [Histonet] Happy Easter! To: Histonet@lists.utsouthwestern.edu Message-ID: <794795.2296...@web112616.mail.gq1.yahoo.com> Content-Type: text/plain; charset=us-ascii Dear histonetters I wish to you, Happy Holidays with a lot of peace and health! dr.Mohamed Abd el razik Histology Dep. Faculty of Vet. Med. Cairo University- Egypt ------------------------------ Message: 4 Date: Mon, 5 Apr 2010 13:47:08 +0100 From: Malika Benatti <malbena...@googlemail.com> Subject: Re: [Histonet] Histobath To: Matthew Mincer <m...@techoneweb.com> Cc: histonet@lists.utsouthwestern.edu Message-ID: <i2yafdd78481004050547jc7ff6082nface8b2e94908...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi Matt, Depending on the wax melting point which should be around 57 degree Celsius, your water bath should be set at 50 degree Celsius. Hope this help. Malika Benatti BSc MIBMS Specialist Biomedical Scientist Great Ormond Street Children Hospital London, WC1N 3JH UK Tel: (+44) 0207 4059200 Ext 5475 ben...@gosh.nhs.uk On Mon, Apr 5, 2010 at 1:37 PM, Matthew Mincer <m...@techoneweb.com> wrote: > Does anyone know the "normal" operating temperature of the Thermo > Histobath? A customer has asked me to repair theirs and I want to make sure > I get the temp correct. > > Thanks > Matt > > -- > Matthew Mincer > Tech One Biomedical Service > 159 N Marion Street > PMB163 > Oak Park, IL 60301 > office (708) 383-6040 X 10 > cell (708) 822-3738 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- " Smile .... it confuses people " ------------------------------ Message: 5 Date: Mon, 05 Apr 2010 07:56:19 -0500 From: Matthew Mincer <m...@techoneweb.com> Subject: [Histonet] Histobath part 2 To: histonet@lists.utsouthwestern.edu Message-ID: <4bb9ddf3.3080...@techoneweb.com> Content-Type: text/plain; charset=ISO-8859-1; format=flowed I forgot to mention, this Histobath I am talking about is the one with a refrigerated tank. You fill the tank with a liquid (I forget what the chemical is) and use it to quick-freeze tissue to be cut in a cryostat. Peace Matt -- Matthew Mincer Tech One Biomedical Service 159 N Marion Street PMB163 Oak Park, IL 60301 office (708) 383-6040 X 10 cell (708) 822-3738 ------------------------------ Message: 6 Date: Mon, 05 Apr 2010 09:03:04 -0400 From: "Lynette Pavelich" <lpave...@hurleymc.com> Subject: Re: [Histonet] Histobath part 2 To: <histonet@lists.utsouthwestern.edu>, "Matthew Mincer" <m...@techoneweb.com> Message-ID: <4bb9a748.59cd.00e...@hurleymc.com> Content-Type: text/plain; charset=US-ASCII Matt, I have a copy of the instructional/operational manual from my histobath that we purchased in 1994 from Shandon-Lipshaw. The specifications for its lowest temperature is -60C. The liquid that is used is 2-methylbutane (isopentane). I can fax you a copy if it would help. Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: lpave...@hurleymc.com ph: 810-257-9948 Lab: 810-257-9138 fax: 810-762-7082 >>> Matthew Mincer <m...@techoneweb.com> 4/5/2010 8:56 AM >>> I forgot to mention, this Histobath I am talking about is the one with a refrigerated tank. You fill the tank with a liquid (I forget what the chemical is) and use it to quick-freeze tissue to be cut in a cryostat. Peace Matt -- Matthew Mincer Tech One Biomedical Service 159 N Marion Street PMB163 Oak Park, IL 60301 office (708) 383-6040 X 10 cell (708) 822-3738 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Mon, 5 Apr 2010 13:59:06 +0100 From: Malika Benatti <malbena...@googlemail.com> Subject: Re: [Histonet] Histobath To: histonet@lists.utsouthwestern.edu Message-ID: <x2lafdd78481004050559lf76cee5fk1677e4f09a6ad...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Sorry for that Matt, In this case the internal temperature of the cryostat chamber should be set at -20 to -22 degree Celsius, not sure what would the actual temperature of the histobath itself but our thermo scientific cryostat has the following settings: - the cryobar is set at -18 Degree Celsius, - the Cryo chamber at - 22 degree Celsius. Hope this help Malika Benatti Malika Benatti BSc MIBMS Specialist Biomedical Scientist Great Ormond Street Children Hospital London, WC1N 3JH UK Tel: (+44) 0207 4059200 Ext 5475 ben...@gosh.nhs.uk - Show quoted text - On Mon, Apr 5, 2010 at 1:51 PM, Matthew Mincer <m...@techoneweb.com> wrote: > Thanks, but the Histobath I was talking about is a freezing device for > cryostats. > > Matt > > Malika Benatti wrote: > >> Hi Matt, >> >> Depending on the wax melting point which should be around 57 degree >> Celsius, your water bath should be set at 50 degree Celsius. >> >> Hope this help. >> >> Malika Benatti BSc MIBMS >> Specialist Biomedical Scientist >> Great Ormond Street Children Hospital >> London, WC1N 3JH >> UK >> >> Tel: (+44) 0207 4059200 Ext 5475 >> ben...@gosh.nhs.uk <mailto:ben...@gosh.nhs.uk> >> >> >> >> >> >> On Mon, Apr 5, 2010 at 1:37 PM, Matthew Mincer <m...@techoneweb.com<mailto: >> m...@techoneweb.com>> wrote: >> >> Does anyone know the "normal" operating temperature of the Thermo >> Histobath? A customer has asked me to repair theirs and I want to >> make sure I get the temp correct. >> >> Thanks >> Matt >> >> -- Matthew Mincer >> Tech One Biomedical Service >> 159 N Marion Street >> PMB163 >> Oak Park, IL 60301 >> office (708) 383-6040 X 10 >> cell (708) 822-3738 >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> <mailto:Histonet@lists.utsouthwestern.edu> >> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> -- >> " Smile .... it confuses people " >> > > > -- > Matthew Mincer > Tech One Biomedical Service > 159 N Marion Street > PMB163 > Oak Park, IL 60301 > office (708) 383-6040 X 10 > cell (708) 822-3738 > > -- " Smile .... it confuses people " ------------------------------ Message: 8 Date: Mon, 5 Apr 2010 08:08:17 -0500 From: "Mahoney,Janice A" <janice.maho...@alegent.org> Subject: RE: [Histonet] 72644.18148...@web111105.mail.gq1.yahoo.com To: 'Joe Nocito' <jnoc...@satx.rr.com>, Andrew Burgeson <nap...@siscom.net>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <8f0ee4144e8e2f4ca1f6b051a2e5bfeea7c4a...@exchmbc2.ad.ah.local> Content-Type: text/plain; charset="us-ascii" Talk about politically incorrect. "Pregnant, out of wedlock 16 year olds?" Are you kidding me? Seriously AB? Jan -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, April 01, 2010 7:42 PM To: Andrew Burgeson; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] 72644.18148...@web111105.mail.gq1.yahoo.com I've seen lab get accredited one month, only to have CLIA come through the next month and rip that lab apart. I have been through CAP inspections that consisted of the inspector looking only at slides while he checked off the checklist to inspectors carrying their own thermometers and testing the temp of the paraffin baths on the tissue processors. This is supposed to be a peer review, often it's a witch hunt. I had one CAP team leader tell me that my lab was the last lab he was going to inspect because he dropped out of CAP and went with JCAHO, which was approved by his hospital administrator. Does anyone know why CAP split the "processing" and "grossing parts anyway? I thought it was a stupid idea in the first place. But then again, I've never been known to be politically correct Joe ----- Original Message ----- From: "Andrew Burgeson" <nap...@siscom.net> To: <histonet@lists.utsouthwestern.edu> Sent: Thursday, April 01, 2010 12:26 PM Subject: [Histonet] 72644.18148...@web111105.mail.gq1.yahoo.com > Sheesh is right, J. > > CAP is all politics as far as I am concerned. It is all > about protecting the careers and paychecks of the general > pathology community. > > I am thouroughly unimpressed with JCAHO, CAP et al. > > If all you need to legally run a laboratory is to be CLIA > inspected, then WHY BOTHER with these subjective entities? > > > The BS I have heard over the last few months concerning MOHS > surgery specimens is one glaring example of the limitations > CAP has in understanding fully certain nuances of the lab > trade. > > Ridiculous. Unless you want the marketing and potential > "perception" that you are better covered from a legal > standpoint, CAP certs are worthless. > > The more I hear about CAP certifications, the more I see it > as a certain community of individuals who are protecting > their perceived "TURF." > > In the end, the pathologists in the group and in the > facility in which you are working have to take > responsibility for these matters. If the docs think a CAP > cert is necessary, then do it and live with it. If not, then > consider yourself lucky to not have to see these people in > your lab. > > I have been through MANY CAP inspections in and out of the > military. For the most part, though, I see people paying > this organization to inspect their lab as the same thing as > "burning a pinch of incense in honor of great Caesar, ruler > of Rome." It will get you some kudos, but tangibly not > change much at all if your pathologists or HR $ hiring hands > want to pocket more $ as a result of hiring "pregnant out of > wedlock 16 year olds" to gross tissue and cut slides. > > Seen it. > > AB > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. 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Thank you for your cooperation. ------------------------------ Message: 9 Date: Mon, 5 Apr 2010 09:00:58 -0500 From: "Bernice Frederick" <b-freder...@northwestern.edu> Subject: RE: [Histonet] RCC To: "'Howery, Jeffrey'" <jhowe...@yrmc.org>, <histonet@lists.utsouthwestern.edu> Message-ID: <6d9b42048b2e43c4aeec2b630377c...@lurie.northwestern.edu> Content-Type: text/plain; charset="US-ASCII" RCC from Novacastra (Leica) Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Howery, Jeffrey Sent: Friday, April 02, 2010 12:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RCC What are people using for a Renal cell Carcinoma. ( this is a test ) Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Mon, 5 Apr 2010 10:06:17 -0400 From: "McMahon, Loralee A" <loralee_mcma...@urmc.rochester.edu> Subject: [Histonet] RE: CD5 To: "Howery, Jeffrey" <jhowe...@yrmc.org>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <c27aa2a01cef31469813089e226f582e838c0...@urmcms7.urmc-sh.rochester.edu> Content-Type: text/plain; charset="us-ascii" High pH Retrieval for 20 minutes in the PT module 20 minute incubation of CD5 15 minute mouse linker 20 Minute incubation Flex HRP 10 minute DAB 5 minute Hematoxylin Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Howery, Jeffrey [jhowe...@yrmc.org] Sent: Friday, April 02, 2010 4:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD5 Can anyone share thier protocol for CD5. We use the Dako platform. Thanks Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Mon, 5 Apr 2010 10:26:08 -0400 From: "Parsons, Catherine" <cpars...@vt.edu> Subject: [Histonet] Choosing Carousel Style Tissue Processors To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <f432a8a667be1a43bc6b09c8611856b425a4c25...@fangorn.cc.w2k.vt.edu> Content-Type: text/plain; charset="us-ascii" I am in the process of selecting a new tissue processor for my lab, and have narrowed the search to two models. Both are carousel style tissue processors. We are a small, low volume research lab, so the carousel style fits well with our processing and space needs. I would appreciate any information/opinions that anyone has about these two models and manufacturers. Major lab equipment purchases seem to be on a 20 year cycle here, so I need to choose carefully and well! My choices are the Leica TP 1020 and the Thermo Scientific Microm STP 120 Spin tissue processor. Has anyone found the spin function on the Microm unit to be useful, or just another part to break down and need replacement? Thanks in advance for any information that you can offer. Cathy Parsons cpars...@vt.edu ------------------------------ Message: 12 Date: Mon, 5 Apr 2010 09:55:30 -0500 From: "Dawson, Glen" <gdaw...@dynacaremilwaukee.com> Subject: [Histonet] RE: CD5 To: <histonet@lists.utsouthwestern.edu> Message-ID: <b3d65550856d0146b900d401ee313d4b03404...@dynams.dynacaremilwaukee.com> Content-Type: text/plain; charset="iso-8859-1" Jeff, Thermo Scientific (Formally NeoMarkers) CD5 (Clone SP19): Catalogue# RM-9119-S. Concentration of 1:75 diluted w/Dako diluent. Antigen Retrieval: PH6.0 Citrate Target Retrieval (DAKO) for 35 min. @ 99 degrees C, followed by a 20 min. room temp. cool down. 35 minute primary antibody application. 30 minute Envision+ polyclonal (DAKO) secondary antibody detection. 7 minute DAB+ (DAKO). Counter-stain as you like. Results on this antibody are excellent. Best of Luck, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Howery, Jeffrey [jhowe...@yrmc.org] Sent: Friday, April 02, 2010 4:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD5 Can anyone share thier protocol for CD5. We use the Dako platform. Thanks Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 77, Issue 5 *************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet