Drew, At one time we were going to bring this stain in-house (Amyloid A) but finding positive control material was problematic. One of our pathologists remembered a case from more than 20 years ago (and had documented it!) but it was still just a tiny renal biopsy. We found that ProPath (214-237-1894, www.propath.com ) does this antibody so we gave them some of our renal biopsy and since then have sent our cases to them for staining.
Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Drew Meyer Sent: Monday, April 05, 2010 1:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Amyloid Stain Hey Guys, I was hoping to get some help with the best way to approach a request for an amyloid stain. The only thing I've ever done in the past is the traditional Congo Red... however we were wondering how the Amyloid A Immuno stain is. Our pathologists were wondering if the Amyloid A is more/less reliable and if there are any limitations associated with this antibody. I just don't know that much about it and was hoping you guys could enlighten me! Thanks for the help! Drew _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet