We use Biocare's Universal Negative Control Serum for our negative controls on the Benchmark XT. I qc the IHC slides daily and don't see any staining in the negative sections. We are a large reference lab and I see lots of negative control slides daily.
You might have gotten a bad batch. I've had much more trouble with Ventana's negative rabbit serum before they finally discontinued it and switched to a new product. Mark Tarango On Wed, Apr 7, 2010 at 1:37 PM, Gagnon, Eric <gagn...@kgh.kari.net> wrote: > Having just validated and started using Biocare Universal Negative Control > Serum, we were surprised when a pathologist brought back two cases in which > tumour and/or epithelium came up positive. This staining was in the same > locations as positive staining with some markers. We use Ventana BenchMark > XT's, and the staining was in both iView and UltraView protocols. > Mechanical problems, contamination and dilution of the Negative Control > Serum did not appear to be factors. > > Biocare knows about this and is meeting with Ventana to iron out the > problem. Would have been nice to know about this if it was a known issue > before we bought and started using the product, but better late than never. > Posting this in case anyone else on Histonet is experiencing similar > staining challenges using this product. As Biocare states in the product > insert sheet, when used in conjunction with non-Biocare kits, results may > vary. We'll keep using it and are currently waiting for advice from > Biocare. > > Eric Gagnon MLT > Histology Laboratory > Kingston General Hospital > Kingston, Ontario, Canada > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet