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Today's Topics:
1. Re: Time allotted for Frozen sections (Rene J Buesa)
2. Double staining of the same Primary (Reuel Cornelia)
3. Uranyl Nitrate (Dawn Oakes)
4. RE: SPAM-LOW: [Histonet] qihc (Patsy Ruegg)
5. RE: anti-F4/80 on IntelliFlex (Patsy Ruegg)
6. Re: Uranyl Nitrate (Rene J Buesa)
7. amyloid ? for Bob Richmond (Carrie Disbrow)
8. TX Society meeting? (Cheryl)
9. Re: amyloid ? for Bob Richmond (Richard Cartun)
10. Re: Cleaning VIP Processor containers (Joseph Saby)
11. Re: Uranyl Nitrate (John Kiernan)
12. Cleaning VIP Processor Containers - Summary (Brandi Higgins)
13. Aniline blue (Itai Moshe)
14. Re: Uranyl Nitrate (Rene J Buesa)
15. picro sirius red, sirius red connective tissue
stain[Scanned] (Itai Moshe)
16. frozen lung sections (Molinari, Betsy)
17. Old Manuals (Michael J. Lyon, Ph.D.)
18. RE: frozen lung sections (Dishop, Megan)
19. herring sperm dna (Emily Sours)
20. RE: Time allotted for Frozen sections (Kathy M. Gorham)
21. abtibodies (Cynthia Pyse)
22. instrument disinfection (Margiotta, Michele)
23. (no subject) (Santiago, Albert)
----------------------------------------------------------------------
Message: 1
Date: Tue, 6 Apr 2010 10:09:27 -0700 (PDT)
From: Rene J Buesa <rjbu...@yahoo.com>
Subject: Re: [Histonet] Time allotted for Frozen sections
To: histonet@lists.utsouthwestern.edu, "Kathy M. Gorham"
<km...@grh.org>
Message-ID: <382702.38475...@web65704.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
CAP requires an explanation as to why any FS diagnosis takes more than 20
minutes after the sample reaches the lab.
The average time is 15 minutes.
René J.
--- On Tue, 4/6/10, Kathy M. Gorham <km...@grh.org> wrote:
From: Kathy M. Gorham <km...@grh.org>
Subject: [Histonet] Time allotted for Frozen sections
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, April 6, 2010, 12:33 PM
Good morning histoland. What is the maximum time allotted for frozen
section per block? Is this written in CAP regs? JCAHO regs?
Thanks, Kathy Gorham H.T.
GRH National Recognition
Outstanding Rural Health Organization of 2009 awarded by NRHA
Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP
Leader in Innovative Excellence 2009 awarded by the OAHHS
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------------------------------
Message: 2
Date: Tue, 06 Apr 2010 13:09:32 -0500
From: "Reuel Cornelia" <reuel.corne...@tsrh.org>
Subject: [Histonet] Double staining of the same Primary
To: <histonet@lists.utsouthwestern.edu>
Message-ID: <4bbb328c020000c500074...@mail.tsrh.org>
Content-Type: text/plain; charset=US-ASCII
Can anybody share their protocol for Rat spinal cord double immunostain
both flourescent and ABC-HRP of the same species (mouse) Primary antibody.
Thank you.
------------------------------
Message: 3
Date: Tue, 6 Apr 2010 11:49:05 -0700
From: Dawn Oakes <doa...@olympicmedical.org>
Subject: [Histonet] Uranyl Nitrate
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Message-ID:
<f5ecf3459f04cb43850d1eac8180a737c3e...@is-210vs.olympicmedical.local>
Content-Type: text/plain; charset="us-ascii"
Is there any substitutes for Uranyl Nitrate in the modified Steiner
Stain? How is it being disposed of?
Dawn Oakes HT
Olympic Medical Center
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------------------------------
Message: 4
Date: Tue, 6 Apr 2010 14:56:52 -0600
From: "Patsy Ruegg" <pru...@ihctech.net>
Subject: RE: SPAM-LOW: [Histonet] qihc
To: "'Sara Baldwin/mhhcc.org'" <sbald...@mhhcc.org>, "'histonet'"
<histonet@lists.utsouthwestern.edu>
Message-ID: <cd8fe87d309e49fd809f7a03f7540...@prueggihctechlt>
Content-Type: text/plain; charset="us-ascii"
I do not believe so. There are some questions from the Self Assessment
booklet from NSH on IHC/EM and Enzyme Histochemistry but since it has so
many subjects to cover not much space left for just IHC. Your best bet is
the Dako Handbook on IHC and the references listed on the IHC Resource
Group
website, www.ihcrg.org , join the group online from the website to access
the references and the pp presentation on Taking the QIHC by Ethel Macrea,
gotta be a member of NSH to join the IHCRG but it is a great resource.
Regards,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net
www.ihcrg.org
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sara
Baldwin/mhhcc.org
Sent: Tuesday, April 06, 2010 8:32 AM
To: histonet
Subject: SPAM-LOW: [Histonet] qihc
Is there a practice test out there for the QIHC?
ThanksPatholgy Supervisor
Kathy Baldwin, SCT (ASCP)
M=morial Hospital and Health Care Center
[1]sbald...@mhhcc.org
Ph& nbsp;812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897
Confidential information, Authorized use only.
References
1. ="mailto:sbald...@mhhcc.org";
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------------------------------
Message: 5
Date: Tue, 6 Apr 2010 14:59:30 -0600
From: "Patsy Ruegg" <pru...@ihctech.net>
Subject: RE: [Histonet] anti-F4/80 on IntelliFlex
To: "'Connolly, Brett M'" <brett_conno...@merck.com>,
<histonet@lists.utsouthwestern.edu>
Message-ID: <3790e77523054a24b4b53f508b3db...@prueggihctechlt>
Content-Type: text/plain; charset="us-ascii"
Brett,
I run F4/80 using the BC rat on ms detection (not their instrument)
without
getting BG issues, are you using Proteinase K digestion? I found that to
work better than HIER.
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net
www.ihcrg.org
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Connolly,
Brett M
Sent: Tuesday, April 06, 2010 9:03 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] anti-F4/80 on IntelliFlex
Is anyone running the Serotec rat anti-mouse F4/80 on the Biocare
IntelliFlex platform? We are having background problems.
Thanks,
Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
tel. 215-652-2501 fax. 215-993-6803
brett_conno...@merck.com
Notice: This e-mail message, together with any attachments, contains
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------------------------------
Message: 6
Date: Tue, 6 Apr 2010 14:01:53 -0700 (PDT)
From: Rene J Buesa <rjbu...@yahoo.com>
Subject: Re: [Histonet] Uranyl Nitrate
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>, Dawn Oakes
<doa...@olympicmedical.org>
Message-ID: <653634.88459...@web65707.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Yes, 1% aq. sol. of phosphotungstic acid.
Want the reference?
René J.
--- On Tue, 4/6/10, Dawn Oakes <doa...@olympicmedical.org> wrote:
From: Dawn Oakes <doa...@olympicmedical.org>
Subject: [Histonet] Uranyl Nitrate
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Date: Tuesday, April 6, 2010, 2:49 PM
Is there any substitutes for Uranyl Nitrate in the modified Steiner
Stain? How is it being disposed of?
Dawn Oakes HT
Olympic Medical Center
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------------------------------
Message: 7
Date: Tue, 06 Apr 2010 17:05:58 -0400
From: "Carrie Disbrow" <dis...@shands.ufl.edu>
Subject: [Histonet] amyloid ? for Bob Richmond
To: <histonet@lists.utsouthwestern.edu>
Message-ID: <4bbb69f6.72ac.005...@shands.ufl.edu>
Content-Type: text/plain; charset=US-ASCII
Hi,
I was also told or read somewhere that insulinomas are good amyloid
controls. I've never had a chance to test any blocks from these cases.
What has your experience been? Thanks for your help.
Carrie
------------------------------
Message: 8
Date: Tue, 6 Apr 2010 14:09:08 -0700 (PDT)
From: Cheryl <tkngfl...@yahoo.com>
Subject: [Histonet] TX Society meeting?
To: histonet@lists.utsouthwestern.edu
Message-ID: <833406.16237...@web50906.mail.re2.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Any word on Texas' regional histology meeting? I'm looking for dates and
the registration info for my team--
Thanks!
Cheryl Kerry, HT(ASCP)
------------------------------
Message: 9
Date: Tue, 06 Apr 2010 17:14:33 -0400
From: "Richard Cartun" <rcar...@harthosp.org>
Subject: Re: [Histonet] amyloid ? for Bob Richmond
To: <histonet@lists.utsouthwestern.edu>, "Carrie Disbrow"
<dis...@shands.ufl.edu>
Message-ID: <4bbb6bf8.7400.007...@harthosp.org>
Content-Type: text/plain; charset=US-ASCII
Yes, insulinomas can show amyloid; however, they are very rare tumors.
Richard
Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596 Office
(860) 545-2204 Fax
"Carrie Disbrow" <dis...@shands.ufl.edu> 4/6/2010 5:05 PM >>>
Hi,
I was also told or read somewhere that insulinomas are good amyloid
controls. I've never had a chance to test any blocks from these cases.
What has your experience been? Thanks for your help.
Carrie
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------------------------------
Message: 10
Date: Tue, 6 Apr 2010 14:57:16 -0700 (PDT)
From: Joseph Saby <saby_josep...@yahoo.com>
Subject: Re: [Histonet] Cleaning VIP Processor containers
To: Anthony Reilly <tony_rei...@health.qld.gov.au>, Brandi Higgins
<brandihigg...@gmail.com>, histonet@lists.utsouthwestern.edu
Message-ID: <276470.25744...@web113805.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Brandi-
The flush should be with warm water (you do not need hot), and should be
for the fixative station(s) and the first alcohol. You only need 1 minute
in each, just enough to get the solution to pump in.
Joe Saby, BA HT
________________________________
From: Anthony Reilly <tony_rei...@health.qld.gov.au>
To: Brandi Higgins <brandihigg...@gmail.com>;
histonet@lists.utsouthwestern.edu
Sent: Mon, April 5, 2010 10:36:42 PM
Subject: Re: [Histonet] Cleaning VIP Processor containers
Hi Brandi
Do you do hot water flushes? If not the film will be a build up of the
buffer salts from your formalin. To flush replace your formalin containers
with hot water and write a program to run each container for 10-15
minutes. This will not only clean the retorts but the fluid lines as well.
This should be done weekly or at least monthly if time is an issue.
regards
Tony
Tony Reilly B.Sc. , M.Sc.
Chief Scientist, Anatomical Pathology
Pathology Queensland-PA Laboratory
_________________________________________________
Clinical and Statewide Services Division| QueenslandHealth
Level 1, Building 15,Princess Alexandra Hospital
Ipswich Road,WOOLLOONGABBA Qld4102
Ph: 07 3176 2412
Mob: 0402 139411
Fax: 07 3176 2930
Email: tony_rei...@health.qld.gov.au
Web: www.health.qld.gov.au/qhcss/
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------------------------------
Message: 11
Date: Tue, 06 Apr 2010 18:44:46 -0400
From: John Kiernan <jkier...@uwo.ca>
Subject: Re: [Histonet] Uranyl Nitrate
To: Rene J Buesa <rjbu...@yahoo.com>
Cc: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Message-ID: <fbc4d4aa16a90.4bbb8...@uwo.ca>
Content-Type: text/plain; charset=iso-8859-1
Yes. Please post the reference.
-
John Kiernan
Anatomy, UWO
London, Canada
= = =
----- Original Message -----
From: Rene J Buesa <rjbu...@yahoo.com>
Date: Tuesday, April 6, 2010 17:03
Subject: Re: [Histonet] Uranyl Nitrate
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>, Dawn Oakes
<doa...@olympicmedical.org>
Yes, 1% aq. sol. of phosphotungstic acid.
Want the reference?
René J.
--- On Tue, 4/6/10, Dawn Oakes <doa...@olympicmedical.org> wrote:
From: Dawn Oakes <doa...@olympicmedical.org>
Subject: [Histonet] Uranyl Nitrate
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>Date: Tuesday, April 6,
2010, 2:49 PM
Is there any substitutes for Uranyl Nitrate in the modified Steiner
Stain? How is it being disposed of?
Dawn Oakes HT
Olympic Medical Center
-----------------------------------------------------------------
--------
Confidentiality Notice: This e-mail message, including any
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prohibited. If you are not the intended recipient, you have
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immediately by reply email and delete/destroy the original and
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may incorrectly identify the sender.
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------------------------------
Message: 12
Date: Tue, 6 Apr 2010 21:21:50 -0400
From: Brandi Higgins <brandihigg...@gmail.com>
Subject: [Histonet] Cleaning VIP Processor Containers - Summary
To: histonet@lists.utsouthwestern.edu, Katelin Lester
<kate...@cuttingedgehistology.com>
Message-ID:
<y2va1cc9adc1004061821y8a04018ve86889891e330...@mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Thank you to everyone who responded. I got some good suggestions which I
plan on trying.
Just incase the responses I have received weren't sent to everyone, and
also
to make an easier-to-read reference for the future -
Dilute acetic acid.
xylene for waxy buildup
Greased Lightning cleaner (store bought)
50/50 bleach mix - let sit for a while.
warm water flushes in fixative and first alcohol
The only response I received before changing the solutions was the dilulte
acetic acid which we tried but did not find effective.
Brandi Higgins
------------------------------
Message: 13
Date: Wed, 7 Apr 2010 12:00:25 +0300
From: Itai Moshe <itai.mo...@mail.huji.ac.il>
Subject: [Histonet] Aniline blue
To: histonet@lists.utsouthwestern.edu
Message-ID:
<i2kb79b24171004070200s2c754d3fn259ea17262397...@mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
What type of collagen aniline blue is staining ?
Thanks in advance
Itai
------------------------------
Message: 14
Date: Wed, 7 Apr 2010 06:35:16 -0700 (PDT)
From: Rene J Buesa <rjbu...@yahoo.com>
Subject: Re: [Histonet] Uranyl Nitrate
To: John Kiernan <jkier...@uwo.ca>
Cc: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Message-ID: <160131.64085...@web65703.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Reference for phosphotungstic acid substituting uranyl nitrate as a
sensitizer:
Journal of Histotechnology, 2001; 24(2):113-116
René J.
--- On Tue, 4/6/10, John Kiernan <jkier...@uwo.ca> wrote:
From: John Kiernan <jkier...@uwo.ca>
Subject: Re: [Histonet] Uranyl Nitrate
To: "Rene J Buesa" <rjbu...@yahoo.com>
Cc: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Date: Tuesday, April 6, 2010, 6:44 PM
Yes. Please post the reference.
-
John Kiernan
Anatomy, UWO
London, Canada
= = =
----- Original Message -----
From: Rene J Buesa <rjbu...@yahoo.com>
Date: Tuesday, April 6, 2010 17:03
Subject: Re: [Histonet] Uranyl Nitrate
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>, Dawn Oakes
<doa...@olympicmedical.org>
Yes, 1% aq. sol. of phosphotungstic acid.
Want the reference?
René J.
--- On Tue, 4/6/10, Dawn Oakes <doa...@olympicmedical.org> wrote:
From: Dawn Oakes <doa...@olympicmedical.org>
Subject: [Histonet] Uranyl Nitrate
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>Date: Tuesday, April 6,
2010, 2:49 PM
Is there any substitutes for Uranyl Nitrate in the modified Steiner
Stain? How is it being disposed of?
Dawn Oakes HT
Olympic Medical Center
-----------------------------------------------------------------
--------
Confidentiality Notice: This e-mail message, including any
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immediately by reply email and delete/destroy the original and
all copies of this communication. Also know that Internet e-
mail is not secure. In choosing to communicate with Olympic
Medical Center by email you will assume these confidentiality
risks. Internet messages may become corrupted, incomplete, or
may incorrectly identify the sender.
_______________________________________________
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------------------------------
Message: 15
Date: Wed, 7 Apr 2010 16:45:46 +0300
From: Itai Moshe <itai.mo...@mail.huji.ac.il>
Subject: [Histonet] picro sirius red, sirius red connective tissue
stain[Scanned]
To: histonet@lists.utsouthwestern.edu, baza0...@d.umn.edu,
david.rushwo...@mail.bhrv.nwest.nhs.uk
Message-ID:
<s2ob79b24171004070645i516ed3a6y7f297eaad7c8e...@mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
picro Sirius red and Fast green protocol:
Sirius red (Sirius Red F3B)- 0.1% in Picric acid.
Fast Green - 0.03% in Picric acid (100%).
Can be re-used, filter twice before each use.
1) Deparaffinization and Rehydration:
A) Xylen - 10 min X2
B) Ethanol 100% - 2 Min X2
C) Ethanol 96% - 2 Min X1
D) Ethanol 80% - 2 Min X1
E) Ethanol 70% - 2 Min X1
2) Rinse in double distilled water (DDW) - 5 Min
3) Rinse in filtered Fast green - 20 Min
4) Wash with DDW ( just fill the slide box with DDW and empty).
5) Rinse in filtered Sirius red - 5 Min.
6) Wash with DDW ( just fill the slides box with DDW and empty).
7) Check in microscope, if the colors are not strong enough, repeat from
step 2 to 7.
That's it.
--
Itai Moshe
Mark Pines lab
Institute of Animal Sciences,Volcani Center.
Dept. of Animal Sciences, School of Veterinary Medicine, The Hebrew
University of Jerusalem
Israel
------Original Message-----
*Adam Bazama* baza0013 <@t> d.umn.edu
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author ]<http://lists.utsouthwestern.edu/mailman/htdig/histonet/2008-October/author.html#39886>
------------------------------
Hi Dave,
I noticed that you posted a request in march of 2004 on histonet for a
Sirius red fast green staining protocol. If you do have a protocol, would
you mind sending it to me. I have had a hard time finding one to compare
to
mine and my lack luster results.
Thank you,
Adam Bazama, B.S.
Junior Scientist
Lillehei Heart Institute Histology and Microscopy Core Facility
University of Minnesota Medical School, Division of Cardiology
4-266 Nils Hasselmo Hall
312 Church Street SE
Minneapolis, MN 55455
Lab Desk: 612-625-6779
Cell: 952-334-0607
--------------------------------------------
*David Rushworth* David.Rushworth <@t> mail.bhrv.nwest.nhs.uk
<histonet%40lists.utsouthwestern.edu?Subject=%5BHistonet%5D%20sirius%20red%20connective%20tissue%20stain%5BScanned%5D&In-Reply-To=>
*Thu Mar 4 09:00:18 CST 2004*
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------------------------------
Can someone please supply method for Sirius red-fast green stain for
connective tissue.
Thanks in anticipation.
Dave.
------------------------------
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------------------------------
Message: 16
Date: Wed, 7 Apr 2010 09:46:20 -0500
From: "Molinari, Betsy" <bmolin...@heart.thi.tmc.edu>
Subject: [Histonet] frozen lung sections
To: <histonet@lists.utsouthwestern.edu>
Message-ID:
<c0b5840f14db5b4abf55d82a623d1e1b2bc...@thimailexch.thi2.com>
Content-Type: text/plain; charset="us-ascii"
Hi all,
I have received some lung samples on OCT. These lungs have not been in
PBS or any fixative. They went directly from dissection into OCT and
frozen.
I cannot get a section. The temp of the cryostat is -20. I have done
some reading and it seems the best results come from immersion in PBS,
then 4% paraformaldehyde then into sucrose. Can anyone clarify the
procedure or offer advice? I am unfamiliar with this technique since
most of my frozen sectioning is heart tissue and it does not present the
same problem as the lung.
Thanks,
Betsy
Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave
MC1-283
Houston,TX 77030-2607
832-355-6524
832-355-6812
------------------------------
Message: 17
Date: Wed, 7 Apr 2010 10:58:08 -0400
From: "Michael J. Lyon, Ph.D." <ly...@upstate.edu>
Subject: [Histonet] Old Manuals
To: <histonet@lists.utsouthwestern.edu>
Message-ID: <00d001cad662$bc6c6580$354530...@edu>
Content-Type: text/plain; charset="us-ascii"
Found a couple of old manuals and will send them if you want them:
Directions for use of 7203 Thomas-Franz Microtome Knife Sharpener
Reference Manual No. 80-A Horizontal Sliding Microtome, Lipshaw
Michael J. Lyon, Ph.D.
Otolaryngology Research Lab
SUNY Upstate Medical University
750 East Adams Street
Syracuse, NY 13210
Voice 315-464-7253
Fax 315-464-5572
------------------------------
Message: 18
Date: Wed, 7 Apr 2010 09:33:07 -0600
From: "Dishop, Megan" <dishop.me...@tchden.org>
Subject: [Histonet] RE: frozen lung sections
To: "Molinari, Betsy" <bmolin...@heart.thi.tmc.edu>,
"histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Message-ID:
<c4052e7223019c4f9c6035e80c6f990eccdcd...@tchexmbccr2.thechildrenshospital.org>
Content-Type: text/plain; charset="us-ascii"
We inflate our lung tissue with a thin OCT compound (50% OCT/ 50% 0.5M
sucrose mixture) using a needle and syringe and then embed the lung in
100% OCT and snap freeze. It helps for interpretation to have open
alveoli and also gives the tissue some stability internally for frozen
sections.
Megan K. Dishop MD
Department of Pathology, B120
The Children's Hospital
13123 E. 16th Avenue
Aurora, CO 80045
Ph: 720-777-4337
Fax: 720-777-7119
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Molinari,
Betsy
Sent: Wednesday, April 07, 2010 8:46 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] frozen lung sections
Hi all,
I have received some lung samples on OCT. These lungs have not been in
PBS or any fixative. They went directly from dissection into OCT and
frozen.
I cannot get a section. The temp of the cryostat is -20. I have done
some reading and it seems the best results come from immersion in PBS,
then 4% paraformaldehyde then into sucrose. Can anyone clarify the
procedure or offer advice? I am unfamiliar with this technique since
most of my frozen sectioning is heart tissue and it does not present the
same problem as the lung.
Thanks,
Betsy
Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave
MC1-283
Houston,TX 77030-2607
832-355-6524
832-355-6812
_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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================================================================================
------------------------------
Message: 19
Date: Wed, 7 Apr 2010 12:10:23 -0400
From: Emily Sours <talulahg...@gmail.com>
Subject: [Histonet] herring sperm dna
To: histonet@lists.utsouthwestern.edu
Message-ID:
<u2sb39794b1004070910p2bf0aa7bn3087f49109988...@mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Hello all!
Roche has decided to stop manufacturing herring sperm DNA. I thought this
would be an easy product to replace but apparently there's roughly ten
bjillion versions of herring sperm DNA. I have no idea whether ours was
sheared, sonicated, molecular biology grade, etc. It just said herring
sperm DNA on the label. Any suggestions on a replacement? We are using
this, btw, in hybridization buffer for RNA in situ on chick embyro
sections
on slides.
I called Roche and asked tech services what their replacement would be,
but
they just said, here's another DNA product which uses the term
hybridization
in its protocol, COT Human DNA, read the tech sheet to see if that's a
good
replacement. Of course, the tech sheet says nothing about whether it
would
be good in in situ hybridization buffer, just that it's good for
suppressing
cross hybridization in DNA microarrays. The really frustrating thing is
their DIG in situ hybridization protocol calls for herring sperm DNA.
Emily
Shall we always be content with the ancient tinned salad of the subsidized
novel? Or the tired ice-cream of poems which cry themselves to sleep in
the
refrigerators of the mind?
-Lawrence Durrell, Clea
------------------------------
Message: 20
Date: Wed, 7 Apr 2010 09:27:47 -0700
From: "Kathy M. Gorham" <km...@grh.org>
Subject: RE: [Histonet] Time allotted for Frozen sections
To: "Rene J Buesa" <rjbu...@yahoo.com>,
<histonet@lists.utsouthwestern.edu>
Message-ID: <db887a3c23a2d742912bd6aaaf17e0da0114a...@is-mail.grh.lan>
Content-Type: text/plain; charset="iso-8859-1"
Thanks, to all you responded to my question about times for FS. CAP says
20 min. and that's what most everyone else said. Kathy
________________________________
From: Rene J Buesa [mailto:rjbu...@yahoo.com]
Sent: Tuesday, April 06, 2010 10:09 AM
To: histonet@lists.utsouthwestern.edu; Kathy M. Gorham
Subject: Re: [Histonet] Time allotted for Frozen sections
CAP requires an explanation as to why any FS diagnosis takes more than 20
minutes after the sample reaches the lab.
The average time is 15 minutes.
René J.
--- On Tue, 4/6/10, Kathy M. Gorham <km...@grh.org> wrote:
From: Kathy M. Gorham <km...@grh.org>
Subject: [Histonet] Time allotted for Frozen sections
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, April 6, 2010, 12:33 PM
Good morning histoland. What is the maximum time allotted for frozen
section per block? Is this written in CAP regs? JCAHO regs?
Thanks, Kathy Gorham H.T.
GRH National Recognition
Outstanding Rural Health Organization of 2009 awarded by NRHA
Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP
Leader in Innovative Excellence 2009 awarded by the OAHHS
GRH Mission
We will ensure ACCESS to superior QUALITY, affordable health care for all
those in need of our services, including underserved populations within
our communities.
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------------------------------
Message: 21
Date: Wed, 7 Apr 2010 12:45:03 -0400
From: "Cynthia Pyse" <cp...@x-celllab.com>
Subject: [Histonet] abtibodies
To: "'Histonet'" <histonet@lists.utsouthwestern.edu>
Message-ID: <000001cad671$abb3db60$031b92...@com>
Content-Type: text/plain; charset="us-ascii"
Hello Histonetters
I am currently looking to add three new antibodies, napsin, D2-40 and
villin. I stain only human FFPE tissue. If anyone has used these
antibodies
I would appreciate your input. Thanks in advance.
Regards
Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cp...@x-celllab.com
------------------------------
Message: 22
Date: Wed, 7 Apr 2010 12:52:07 -0400
From: "Margiotta, Michele" <mmargio...@bmhmc.org>
Subject: [Histonet] instrument disinfection
To: <histo...@pathology.swmed.edu>
Message-ID:
<d50747366fbe7f498009496aca2d9bb00d4...@bmh-exchange.bmhmc.org>
Content-Type: text/plain; charset="iso-8859-1"
Hi All,
I need to order disinfecting solution for our grossing instruments. I
would like to know what product most labs are using?
Thanks for the info.
Michele Margiotta
BMHMC
Histology Supervisor
631-654-7192
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------------------------------
Message: 23
Date: Wed, 7 Apr 2010 12:56:55 -0400
From: "Santiago, Albert" <albert.santi...@uphs.upenn.edu>
Subject: [Histonet] (no subject)
To: <histonet@lists.utsouthwestern.edu>
Message-ID:
<bb0f4d2041eed9458ad95a538dbc4cae07080...@uphsmbx6.uphs.upenn.edu>
Content-Type: text/plain; charset="us-ascii"
Hello fellow histonetters, we're in the process of researching bar
coding/specimen tracking system for our lab and I was wondering if
anyone had any recommendation of any particular system and/or vendor
that I should look into. Thank you
Albert Santiago, HT(ASCP)
Laboratory Manager
Dermatopathology
215-662-6759/6539-office
215-662-6150-fax
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------------------------------
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End of Histonet Digest, Vol 77, Issue 8
***************************************
