Hi Histonet, I am currently using the protocol from Stainsfile for staining heart tissue: http://stainsfile.info/StainsFile/stain/conektv/masson.htm
My lab had Fast Green readily available so I substituted it for Light Green in Solution C. The slides have not been coming out well. I failed to account for the difference in timing between FG and LG - running the slide in FG for the full 10 minutes. Has anyone used FG in Masson's Trichrome? If so, how long did it take to get the desired colors in the solution? Or, is Fast Green a poor substitute for Light Green? Thanks for your advice! -Colin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
