Hi everyone,
I've been performing Gomori's reticulin impregnation and never got the results
I see in textbooks. The reticulin fibers stain fine, but I get a dark
purple/gray background instead of a light pink background. Does anyone know why
?
Here is the technique I use :
1) Deparaffinize and rehydrate
2) 0,5% potassium permanganate 1 min
3) Running tapwater 3 min
4) Distilled water
5) 5% Oxalic acid 2 min
6) Running tapwater 3 min
7) Distilled water
8) 2% ammonium iron sulfate 1 min
9) Running tapwater 3 min
10) Distilled water
11) Ammoniacal silver 1 min
12) Distilled water 15 sec
13) 20% Formaldehyde 3 min
14) Running tapwater 3 min
15) Distilled water
16) 0,2% Gold chloride 5 min
17) Distilled water
18) 3% potassium metabisuphite 5 min
19) Distilled water
20) Sodium thiosulfate 1 min
21) Running tapwater 3 min
22) Distilled water
23) 0,1% Nuclear fast red 15 min
24) Distilled water
25) Dehydrate, clear and mount
Thanks for your help!
Andre
_________________________________________________________________
Videos that have everyone talking! Now also in HD!
http://go.microsoft.com/?linkid=9724465_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
