Dear Histonetters, We use an Eosin Y/Erythrosin B mixture containing calcium chloride in an aqueous solution, and this, if differentiated correctly in water, gives 3 definite shades of pink. Our students are asked to find an artery to check their differentiation and they should be able to see muscle, connective tissue and red blood cells in three shades of pink.
This solution containing calcium chloride does need to be filtered before use. Regards, Laurie. Mr. Laurie REILLY Histopathology School of Veterinary and Biomedical Sciences James Cook University Townsville Qld. 4811 Australia. Phone 07 4781 4468 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Monday, 19 April 2010 4:38 AM To: 'Kalleberg, Kristopher'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] hematoxylin-eosin saffron In a good H&E, the eosin/phloxine is supposed to be 3 shades: - RBC's/eosinophils/Paneth cells the deepest shade of pink - muscle medium shade of pink - collagen/connective tissue lightest shade of pink Unfortunately, if the eosin and/or phloxine and/or eosin/phloxine does not have the correct concentration of the eosin, pH of eosin, time in alcohols after eosin, etc, there ends up being 2 shades of eosin, with the collagen/CT and the muscle being the same shade. Adding saffron in the HPS gives the collagen/CT a more yellowish shade. So the tissue results are: - RBC's/eosinophils/Paneth cells the deepest shade of pink - muscle medium shade of pink - collagen/connective tissue yellowish with a tinge of pink Therefore, with the HPS, it's easier to differentiate muscle from collagen/CT. But I think most pathologists can diagnose with only 2 shades of eosin. If they need to differentiate collagen/CT from muscle (cirrhosis, tumors), they either ask for a trichrome or IHC. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospitals Royal Oak, MI 48073 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher Sent: Friday, April 16, 2010 1:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] hematoxylin-eosin saffron Can someone please explain to me the purpose of a hematoxylin-eosin saffron stain. Why would this be used over the normal hematoxylin and eaosin stain. Thank you in advance. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet