Ashley,
Phosphatases are relatively resistant to fixation which is why the
histochemical phosphatase methods work. You may want to try putting
phosphatase inhibitors in you fix or post-fix solutions to stop
dephosphorylation, and see if it makes a difference in your ihc. I
think Sigma has a table of reagents somewhere on their website and I'll
email the link if I can find it.
Good luck,
Mike
UF Pharmacology & Therapeutics
------------------------
Date: Fri, 16 Apr 2010 14:43:15 -0500
From: "Troutman, Kenneth A" <kenneth.a.trout...@vanderbilt.edu>
Subject: [Histonet] Phospho antibodies and fixation
To: "Histonet@lists.utsouthwestern.edu"
<Histonet@lists.utsouthwestern.edu>
Message-ID:<7b310892042da74cb3590053f424cfe60b7480e...@its-hcwnem06.ds.vanderbilt.edu>
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Does anyone have any information on phospho antibodies and fixation?...
Thanks,
Ashley Troutman BS, HT(ASCP) QIHC
Vanderbilt University Histopathology
1301 Medical Center Drive TVC 4532
Nashville, TN 37232
615-343-9134
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