Hi Sharon,
1. Stuff the tissue in a cryovial or wrap it in foil and throw it in liquid
N.
2. Put the tissue in a cryomold with OCT covering it and either place it on
some frozen isopentane; put it in a slurry of isopentane and dry ice
pieces; or suspend over liquid N vapors to freeze.
Not sure if this is what you were looking for, precisely?
Regards,
Merced
--On Thursday, April 22, 2010 11:06 AM -0400 "Shaw, Sharon"
<shs...@wpi.edu> wrote:
Does anybody have a protocol on freezing rat heart that you can share
with me. Thanks,
Sharon
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Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
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lei...@buffalo.edu
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