In those situations you can set up your processors to hold in formalin until the time limit is reached and then switch to 70% Sample comes in on Friday. Place it in formalin (on the processor) until the 48 hours have been reached most likely Sunday afternoon, then switch to an extended 70% alcohol, then continue to process so your cassettes will come off on Monday morning. It might mean that you have a 6-8 hour 70% step, but it should be ok. OF COURSE, run some test tissues first to make sure.
Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: Mike Pence [mpe...@grhs.net] Sent: Friday, April 23, 2010 10:58 AM To: McMahon, Loralee A; Patsy Ruegg; Morken, Tim; Maxim Peshkov Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines My problem is that we do not work weekends. If I have a breast that comes in on Friday afternoon, I have to cut it in and have it embedded on Sat. morning. If the guidelines are 72 hr for everything then I can process it on Mondays. -----Original Message----- From: McMahon, Loralee A [mailto:loralee_mcma...@urmc.rochester.edu] Sent: Friday, April 23, 2010 9:22 AM To: Mike Pence; Patsy Ruegg; Morken, Tim; Maxim Peshkov Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines I am not sure what the problem is here. You have to fix for up to 72 hours no more you can do less time. If you are already fixing your her2 for 48 hours, then what needs to change? You are not going to submit different cassettes for Er/Pr to fix for longer? You will submit them all and fix them all for 48 hours max. The guideline is to prevent under AND over fixation. Not to submit two different samples to fix for different times. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence [mpe...@grhs.net] Sent: Friday, April 23, 2010 10:16 AM To: Patsy Ruegg; Morken, Tim; Maxim Peshkov Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines This was my first post about this issue: (I got no responses for) Yes, the paper is there, however, I see they addressed the fixation time in NBF for er/pr but I do not see where they talk about the time changing for her2. So does this mean that I have to fix some of the tumor for her2 for only 48 hours, while the er/pr can be fixed 72 hours? I am not sure why CAP would not address this up front. This is a big problem in our lab. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Friday, April 23, 2010 8:26 AM To: 'Morken, Tim'; 'Maxim Peshkov' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines Dr. Hammond presented the new guidelines for ER (it is just ER not PR as I recall) at the AIMM meeting in Florida at the end of January this year and the fixation times for Breast have been extended to 72 hours, it is supposed to be changed to 72 hours for Her2 as well but I do not know if that has happened yet or not. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Thursday, April 22, 2010 11:06 AM To: Maxim Peshkov Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new er pr cap guidelines Use this link to go to the CAP website to download the 2010 ER/PgR guidelines article (Arch Pathol Lab Med. 2010;134:E1-E16)from the CAP download page: http://tinyurl.com/34kxhtn Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maxim Peshkov Sent: Thursday, April 22, 2010 9:55 AM To: anita dudley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] new er pr cap guidelines Anita: Maybe your pathologist mean this recommendations: "Consensus Recommendations on Estrogen Receptor Testing in Breast Cancer By Immunohistochemistry" (Appl Immunohistochem Mol Morphol 2008;16:513-520)? These recommendations came not from CAP. It is refer only to ER, but not PR. Sincerely, Maxim Peshkov, Russia, Taganrog. ---Original message--- > Date: Tue, 20 Apr 2010 09:46:43 -0500 > From: anita dudley <azdud...@hotmail.com> > Subject: [Histonet] new er pr cap guidelines > To: <histonet@lists.utsouthwestern.edu> > Message-ID: > <snt122-w5922be5ac0a3d838f5b6ebd1...@phx.gbl> > Content-Type: text/plain; charset="iso-8859-1" > > > has anyone seen the new er pr guidelines for er and > pr? my pathologist just came through all in a tizzy > about it. just wondering if anyone else has seen it. > the fixation times were also suppose to change, to > 72 hrs fixation for breast. > > > > thanks for anybodies input, anita, providence hosp. mobile alabama -- С уважением, Maxim mailto:maxim...@mail.ru _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet