Margaret:
We uses 1% aqueous neutral red as red counterstain
with very good results.
Do not forget rinse slides at 10-15 secs in DW
with 1-2 drop of glacial acetic acid before neutral
red and after this, because this dye have red color
at pH < 6.
Sincerely,
Maxim Peshkov,
Russia,
Taganrog.
---Original message---
> Date: Fri, 23 Apr 2010 16:25:30 -0500
> From: "Perry, Margaret" <margaret.pe...@sdstate.edu>
> Subject: [Histonet] Gram stain
> To: "histonet@lists.utsouthwestern.edu"
> <histonet@lists.utsouthwestern.edu>
> Message-ID:
>
> <fca5ef47f9bc694cbb4c58fea04219634ccfb69...@sdsu-mbx.jacks.local>
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>
> Now that I'm done with my rant I have a real
> question. We are trying to do a gram stain on fish
> and the safranine O is staining everything red. What
> other stain would you use? I usually have time to
> look at the books but unfortunately it's almost
> quiting time and the slides need to be done by noon Monday. Thanks
> Margaret Perry
mailto:maxim...@mail.ru
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