Mary, Thanks for the suggestion. We do check the alcohols with a hydrometer and if not 100%, we will use that alcohol as the base for dilution alcohols. We check quality of the xylene by putting 10ml of water in a graduated cylinder and then add 10ml of the recycled xylene to it and shake it vigorosly. Good, clean xylene should have a perfect separation and no "mixing" with the water.
Lynette, I am leaning towards the processing too, but which part?? The clot seems to be more dry than moist, so it's not under dehydrated. Fixation? It spends 3 hours in formalin on the tissue processor and whatever time it can be in formalin prior to the processor. Paraffin impregnation? About the only thing I could think of here was if the wax wasn't changed fast enough and/or we got too much carry over from the xylene. Could that cause this? Thanks for the help! Michelle -----Original Message----- From: Lynette Pavelich [mailto:lpave...@hurleymc.com] Sent: Tuesday, April 27, 2010 6:56 AM To: histot...@imagesbyhopper.com; histonet@lists.utsouthwestern.edu; MARY HODGES Subject: RE: [Histonet] Bone Marrow Clots - falling off slides When I start losing tissue on the slide, I have found that it is usually a processing issue. Is the clot too thick? Try cutting them in half before processing. Hope this helped, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: lpave...@hurleymc.com ph: 810-257-9948 Lab: 810-257-9138 fax: 810-762-7082 >>> MARY T HODGES <hodges...@msn.com> 4/27/2010 12:18 AM >>> recycled anything is not back to it original state always use a hydo meter and add new to back product of a better quality you will find this also with alcohols > From: histot...@imagesbyhopper.com > To: histonet@lists.utsouthwestern.edu > Date: Mon, 26 Apr 2010 23:26:43 -0400 > Subject: [Histonet] Bone Marrow Clots - falling off slides > > Hi Histonetters! > > We have started to have an issue with our bone marrow clots falling off the > slides. We are using plus slides, making sure they drain well (just like > our other slides), but when we stain them routinely, we are getting a fair > amount of tissue coming off the slides. > > It has been suggested that it's related to our using recycled xylene. Does > anyone have any experience with recycled xylene and this type of tissue > fall-off? > > Another suggestion was that the tissues sat in xylene too long. I don't see > how that could happen, under routine conditions, but is that a possibility > for causing this? > > All thoughts will be appreciated! > > Michelle > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:W L:en-US:WM_HMP:042010_1_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.437 / Virus Database: 271.1.1/2835 - Release Date: 04/25/10 18:31:00 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet