I stain derm tissue. We do H&E only using a linear stainer. Two of my docs seem to say that stain is very eosinophilic & smudgy. Other Two say not enough Eosin. Of course!! Followed is my stain protocol. This week I have eliminated the Define and slides seem to not look as eosinophilic and I have not heard any negative remarks but it may be to soon. Any one have any suggestions to change???
Sub X SubX Sub X !00% Alc 100% Alc 100& Alc 95% Alc 95% Alc Water Hemat Hemat Hemat Water Water Define Water Blue Water 95% Alc Eosin 95% Alc 100% Alc 100% Alc SubX Sub X V.Avalos ADS, INC Fax:602-277-2134 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
