I had one of my techs come to me one day saying that she thought I needed to look at her microtome because she had not been able to cut anything on her microtome today. She thought something had gone wrong with the tissue during processing. The other techs though were sectioning fine. This tech has been with me for over 20 years. I went over and looked at her microtome and told her that it might improve if she tried a blade in the blade holder to start.
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liebig, Tyler K. Sent: Monday, May 03, 2010 7:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 78, Issue 2 That is a funny story, I would like to see the slide that was cut from a "blade holder section" with no blade in the clamp. I have heard it can be done but never see it myself. Though in old blade holders with loose clamps I have seen users get two blades in there at once. That didn't section to well either. Tyler -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Sunday, May 02, 2010 10:04 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 78, Issue 2 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Revalidation (Tench, Bill) 2. RE-hydrating air dried slides for Pap stain (Jeffrey Silverman) 3. RE: H. D., Vol 78, Issue 1, Message: 7 (Keith) 4. RE: disposable blade holder (histot...@imagesbyhopper.com) 5. RE: Slide and Block Storage (histot...@imagesbyhopper.com) ---------------------------------------------------------------------- Message: 1 Date: Sat, 1 May 2010 10:37:02 -0700 From: "Tench, Bill" <bill.te...@pph.org> Subject: [Histonet] Revalidation To: histonet@lists.utsouthwestern.edu Message-ID: <2820431bf953bb4da3e9e1a5882265fd02863...@mail1.pph.local> Content-Type: text/plain; charset=us-ascii Kevin, trust your judgment. You have made significant changes in your protocol. You need to revalidate. (and frankly, most changes in a standard protocol would require revalidation). Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 mail2.pph.org made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- ------------------------------ Message: 2 Date: Sat, 1 May 2010 11:43:23 -0700 (PDT) From: Jeffrey Silverman <pathmas...@yahoo.com> Subject: [Histonet] RE-hydrating air dried slides for Pap stain To: vrodrigue...@gmail.com Cc: histonet@lists.utsouthwestern.edu Message-ID: <789166.47922...@web111111.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Valerie, It sounds like you are performing a Pap stain using saline baths to RE-hydrate air dried smears prepared at the bedside, I assume for? staining with Diff Quik stains. The rehydration ( not dehydration) takes place in the sodium chloride baths and restores the morphology of the cells to something closer to a wet fixed state. This takes place in 30 seconds.? You then must actually fix the? now rehydrated cells in 95% alcohol. This step should be of sufficient time to completely fix the cells as one would from the start, up to 20 minutes is needed. . Next the cells need to be rehydrated again, just placed in a water rinse, before staining with hematoxylin and proceeding with the Pap cytoplasmic stains. Awareness of these steps should obviate any problems. Finally, I wouldn't judge the success or failure of the stain by the color of the slide since different tissue fluid backgrounds and amounts of lysed blood might stain differently.? Check the slide microscoipically to assess the stain. Jeff Silverman ------------------------------ Message: 3 Date: Sat, 1 May 2010 12:05:58 -0700 From: "Keith" <mik...@pacbell.net> Subject: [Histonet] RE: H. D., Vol 78, Issue 1, Message: 7 To: <histonet@lists.utsouthwestern.edu> Message-ID: <nlenijcpkpomiabjidgiieebggab.mik...@pacbell.net> Content-Type: text/plain; charset="us-ascii" Here's a copy of a procedure that provided consistently adequate results from my old one-man lab histo days... For your case, perhaps the slides in question were fixed differently or improperly(?) I would pull the questionable slides, delicately rehydrate them, run a control slide, and reprocess them with the following procedure. In this procedure, the staining agents, dehydrants, and clearing agents and can be modified to your facility and reagent list. Keith M. Mikoff, HTL/HT (ASCP) PAPANICOLAOU STAIN (PAP) PURPOSE: The following staining procedure is to be performed only on spray fixed slides. It is a routine stain used for any cytol- ogy (i.e., PAP smears, FNA procedures, or specimens of a fluid nature where cytology is required). PROCEDURE: 1. Dehydrate slides in 2 changes of isopropyl alcohol, 5 minutes each. 2. Rinse in tap water for two minutes. 3. Stain in Harris' Hematoxylin for 2 minutes. 4. Rinse in fresh tap water for 3 minutes. 5. Place in ammonia water for 1 minute. 6. Rinse in fresh tap water for 1 minute. 7. Dehydrate in 3 changes of Isopropyl alcohol, 2 minutes each. 8. Stain in O-G 6 for one minute. 9. Dehydrate in Isopropyl alcohol for 2 changes, 2 minutes each. 10. Stain in EA-50 for 4 minutes. 11. Place in Isopropyl alcohol, 3 changes, at 1 1/2 minutes each 12. To clear the slides, place in 3 changes of Histoclear, for 2 minutes each, and coverslip. ------------------------------ Message: 4 Date: Sun, 2 May 2010 09:01:04 -0400 From: <histot...@imagesbyhopper.com> Subject: RE: [Histonet] disposable blade holder To: <histonet@lists.utsouthwestern.edu> Message-ID: <1729e60b0b6d456bb67e686c815cc...@hopperpc> Content-Type: text/plain; charset="US-ASCII" As a comical sidelight to the last sentence that Tyler wrote ... I have a pathologist who was very upset that our cryostat didn't seem to be working properly. I rushed up to the frozen room to assist and he said, "well, nevermind, I got a slide and diagnosis." I looked in the cryostat and said to him "you actually got a section and were able to render a diagnosis?" "yes" he said. "WOW!, you must be the ONLY pathologist who is capable of sectioning tissue without a knife ..." ;o) He sectioned the tissue and truly had no idea there wasn't a knife in the blade holder! :o) -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liebig, Tyler K. Sent: Friday, April 30, 2010 3:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] disposable blade holder Hello Lucy, In regards to your trouble finding an HM310 blade holder we do have one in our refurbished pool that I could have sent to you. It is used but in working order so we can let you have it at no charge. If you let me know where to ship it I will have it sent. Hope that helps, It's hard to cut without a blade holder. Regards, Tyler Liebig Product Manager Histology Instrumentation and IHC Anatomical Pathology Thermo Fisher Scientific 46360 Fremont Blvd Fremont, CA 94538 USA Office: (510) 979-5000 Mobile: (510) 299-1751 Fax: (510) 979-5239 tyler.lie...@thermofisher.com<mailto:tyler.lie...@thermofisher.com> www.thermo.com<http://www.thermo.com> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.437 / Virus Database: 271.1.1/2845 - Release Date: 04/30/10 11:34:00 ------------------------------ Message: 5 Date: Sun, 2 May 2010 09:04:16 -0400 From: <histot...@imagesbyhopper.com> Subject: RE: [Histonet] Slide and Block Storage To: <histonet@lists.utsouthwestern.edu> Message-ID: <7180df002734414d8feee9a4a4600...@hopperpc> Content-Type: text/plain; charset="US-ASCII" In our facility, we maintain both the blocks and slides. We also store older blocks/slides in a climate controled an off-site facility. Michelle -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Kronenberger Sent: Friday, April 30, 2010 11:00 AM To: 'Nails, Felton'; 'Daniel Adams'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide and Block Storage Clients fax orders for deepers and special stains which we perform and send them out with their next package for them to read and report. -----Original Message----- From: Nails, Felton [mailto:flna...@texaschildrens.org] Sent: Friday, April 30, 2010 10:48 AM To: 'Elizabeth Kronenberger'; 'Daniel Adams'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide and Block Storage So what if the reporting company need to order special stains, it adds too much time having to request the blocks or additional unstained slides. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Kronenberger Sent: Friday, April 30, 2010 9:40 AM To: 'Daniel Adams'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide and Block Storage If our lab does slide preps for other clients (Technical component only), we hold the blocks at our facility. The slides are read and reported by the client, they hold the slides. Liz -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Daniel Adams Sent: Friday, April 30, 2010 12:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide and Block Storage My laboratory provides professional services to some clients and technical services to others. The question has recently come up, who should store blocks and slides when the pathologist and histology laboratory are different companies? - Dan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ ---- -- CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ======================================================================== ==== == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.437 / Virus Database: 271.1.1/2845 - Release Date: 04/30/10 11:34:00 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 78, Issue 2 *************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
